In Vivo Antimicrobial Effect Of 5.25% Sodium Hypochlorite In Root Canals Treatment In Duhok City (original) (raw)
Related papers
The Internet journal of microbiology, 2019
Aim: This study aims to evaluate the bacterial reduction after conventional root canal treatment using 5.25% sodium hypochlorite. Materials and Methods: The study criteria included 100 patients requiring root canal treatments due to pulpitis. The diagnosis was made based on objective clinical signs (examination, probing, percussion, palpation) and X-ray investigation. The clinical procedures were carried out under strict aseptic precautions. All patients were subjected to biomechanical preparation followed by irrigation using sodium hypochlorite at a concentration of 5.25% which is considered the highest concentration without causing any toxic effect on the patient. All microbiological samples were taken immediately after the access preparation baseline (S1) and after the completion of the root canal instrumentation and disinfection with 5.25% sodium hypochlorite (S2) and sent for microbiological analysis. Results: One hundred patients with infected root canal were selected for this...
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2009
The purpose of this study was to determine the antimicrobial efficacy of sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in human root canals infected by Enterococcus faecalis. One hundred sixty-five human single-rooted teeth were prepared and inoculated with E. faecalis for 48 h. Teeth were divided into 3 experimental groups according to the irrigation pattern used: group 1, 4.2% NaOCl pH 12; group 2, 4.2% NaOCl pH 7.5; and group 3, 4.2% NaOCl pH 6.5. Samples from the root canals were collected, and bacterial growth was analyzed by turbidity of the culture medium. None of the irrigating solutions used in this study demonstrated 100% effectiveness against E. faecalis. The antibacterial effectiveness of 4.2% NaOCl at pH 6.5 was significantly increased (P = .03) compared with 4.2% NaOCl at pH 12 (chi-squared test: P < .05). Bactericidal activity of NaOCl solution is enhanced by weak acidification of 4.2% NaOCl solution at pH 6.5.
International Endodontic Journal, 2019
AimTo test the hypothesis that in the daily routine of a specialist clinic in endodontics that irrigation during root canal preparation with 3.0% NaOCl will result in fewer postoperative samples with cultivable bacteria than irrigation with 0.5% buffered NaOCl but, at the same time, will not result in a higher frequency of postoperative pain nor swelling.MethodologyTwo hundred ninety‐eight patients were enrolled in the study and were randomly assigned into two groups – 0.5% NaOCl and 3% NaOCl. All endodontic diagnoses were included. Root canal treatment was performed, and bacterial sampling was carried out prior to root filling. The patients were requested to complete a form regarding pain and swelling seven days postoperatively. Fisher’s exact test, Mann–Whitney U‐test, Mantel–Haenszel chi‐squared and the chi‐squared test with a significance level of P < 0.05 were used for statistical analysis. Multivariable logistic regression was used to adjust for confounders.ResultsIn the 0....
Journal of Applied Oral Science, 2009
OBJECTIVE: The purpose of this study was to evaluate the action of sodium hypochlorite (NaOCl) associated with an intracanal medication against Candida albicans and Enterococcus faecalis inoculated in root canals. MATERIAL AND METHODS: Thirty-six human single-rooted teeth with single root canals were used. The canals were contaminated with C. albicans and E. faecalis for 21 days and were then instrumented with 1% NaOCl. The roots were divided into 3 groups (n=12) according to the intracanal medication applied: calcium hydroxide paste, 2% chlorhexidine (CHX) gel, and 2% CHX gel associated with calcium hydroxide. The following collections were made from the root canals: a) initial sample (IS): 21 days after contamination (control), b) S1: after instrumentation, c) S2: 14 days after intracanal medication placement; S3: 7 days after intracanal medication removal. The results were analyzed statistically by the Kruskal-Wallis test at 5% significance level. RESULTS AND CONCLUSIONS: Both 1% NaOCl irrigation and the intracanal medications were effective in eliminating E. faecalis and C. albicans inoculated in root canals.
Antifungal effects of sodium hypochlorite and chlorhexidine in root canals
Journal of Endodontics, 1999
The purpose of this study was to evaluate the antifungal properties of 0.12% chlorhexidine, 1% NaOCI, and 5% NaOCI. Root sections were enlarged and the smear layer was removed in half of the specimens. The specimens were fixed in the wells of tissue culture plates. Each root canal was dispensed with an inoculum of Candida albicans. After 10 days, the root sections were treated with 3 ml of either disinfectant solution for I rain, 5 min, 30 min, and 1 h. Then, root sections were incubated in test tubes having Sabouraud's Dextrose Broth at 37°C for 24 h. In the presence of the smear layer, antifungal activity was observed only in 1-h treatment groups for all solutions. However, in the absence of the smear layer, 5% NaOCI alone started to show antifungal activity after 30 min. The antimicrobial effectiveness of irrigating solutions should be re-evaluated, particularly in patients predisposed to oral candidiasis.
Journal of Functional Biomaterials
The aim of endodontic therapy is to use various antimicrobial medications for proper cleaning and shaping to create an environment free of microorganisms by eradicating as many as possible from the root canal space. Even although it is a gold standard irrigant, sodium hypochlorite (NaOCl) is known for its cytotoxic effects on vital periapical tissues, making its higher concentrations inappropriate for use in conditions such as wide, underdeveloped, or damaged apices and in cases of perforations. Consequently, if it is ascertained that a gel form of sodium hypochlorite has equivalent antibacterial activity to the aqueous solution form, it could be employed in such situations. The aim of this study was the microbiologic evaluation of 5.25% sodium hypochlorite gel and aqueous solution as root canal disinfectants in multirooted teeth with primary endodontic lesions. Following ethical approval and CTRI registration, 42 patients who gave their consent and had multirooted teeth with pulpal...
International Endodontic Journal, 1997
The effectiveness of 4.0% sodium hypochlorite (NaOCl) used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal was tested in vitro. Root canals contaminated with E. faecalis were treated as follows: (i) irrigation with 2 mL of NaOCl solution and agitation with hand files; (ii) irrigation with 2 mL of NaOCl solution and ultrasonic agitation; (iii) irrigation with NaOCl alternated with hydrogen peroxide. Contaminated canals irrigated with sterile saline solution served as the control. Paper points used to sample bacteria from the root canals were transferred to tubes containing 5 mL of brain heart infusion (BHI) broth. Tubes were incubated and the appearance of broth turbidity was indicative of bacteria remaining in the root canal. There were no statistically significant differences between the experimental groups. However, NaOCl applied by the three methods tested, was significantly more effective than the saline solution (control group) in disinfecting the root canal.
Iranian endodontic journal, 2016
The purpose of this study was to compare the antimicrobial efficacy of sodium hypochlorite (SH) and calcium hypochlorite (CH) against Enterococcus faecalis (E. faecalis) using quantitative real-time polymerase chain reaction (qPCR) analysis and also to compare their cytocompatibility on L929 murine fibroblasts using Mossman's tetrazolium toxicity (MTT) assay. A broth micro-dilution susceptibility test was used to determine the minimum inhibitory concentration (MIC) of each irrigant against E. faecalis. Then, the root canals of 50 mature extracted human mandibular premolars were contaminated with E. faecalis and were randomly divided into three groups according to the irrigant used (n=20). Canals were irrigated with SH in group I (n=20) and CH in group II (n=20) at their obtained MIC. In group III (n=10), sterile saline was used. Microbial sampling was performed before and after biomechanical preparation. Quantitative PCR was used to quantify E. faecalis in the root canal samples...
ABSTRACT: Sodium hypochlorite (NaOCl), a reducing agent, is a clear, straw coloured solution containing about 5% available chlorine. It is the most widely used irrigating solution. NaOCl on ionization produces hypochlorous acid and hypochlorite ion. These are responsible for the antimcrobial ability of the same. No unanimity of opinion exists as to which concentration of NaOCl can be used in root canal therapy. On the basis of published data, 0.5- 5.2% solution is an effective concentration for the use as an irrigant in the root canal. If the canal is filled with the solution during the entire cleaning and shaping procedures, the irrigant will act as an lubricant, solvent to the pulpal tissues and a potent antimicrobial of a no. of solution studied , none was more effective than 5.2% solution of NaOCl. However 2.5% concentration of the same is commonly employed as it decreases the potential for toxicity while maintaining some tissues dissolving and antimicrobial activity. The following article deals with the various accidents caused by misuse of sodium hypochlorite and their management. KEY WORDS: Accident, irrigation, sodium hypochlorite(NaOCI), prevention. | Dr VINEET VINAYAK | dr VINEET vinayak
Journal of dental research, dental clinics, dental prospects, 2017
The aim of this study was to evaluate the effectiveness of different concentrations of sodium hypochlorite (NaOCl) solution in reducing bacterial growth in Enterococcus faecalis biofilms in root canals. The root canals of maxillary central incisors of 104 subjects underwent chemomechanical debridement. In order to remove the smear layer, 5.25% sodium hypochlorite solution was used for 3 minutes in the root canals. Then, the samples were immersed in 1 mL of 17% EDTA for 3 minutes. Finally, the root canals were irrigated with phosphate-buffered saline (PBS) solution. After removing the smear layer, the samples were sterilized. Then E. faecalis biofilms formed within the root canals at 4-, 6-, and 10-week intervals were evaluated. Each group was divided into 4 subgroups in terms of the antibacterial treatment: group 1: 1% NaOCl solution; group 2: 2.5% NaOCl solution; group 3: 5.25% NaOCl solution; and group 4: PBS solution. After preparation of root canal filings, the counts of live ba...