MYCOBIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANO PARTICLES AND ITS ANTIMICROBIAL ACTIVITY (original) (raw)
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IJMTST, 2020
Extracellular biosynthesis of silver nanoparticles by fungus Aspergillus niger which was isolated from waste water is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-Vis spectrum, showing the absorbance between 260 to 400 nm. The nanoparticles characterized by Scanning Electron Microscopy exhibited silver nanoparticles with diameter of 25nm to 75nm. Energy Dispersive X-ray analysis reveals strong signals in the silver region and confirms the formation of the silver nanoparticles. The Fourier Transform Infrared Spectroscopy study confirmed that the A. niger mycomass has the ability to perform both reduction and capping functions on the silver nanoparticles. Compound Microscopy confirms the self-assembling property of silver nanoparticles. The silver nanoparticles showed remarkable antibacterial activity against Lactobacillus plantarum, Lactobacillus delbrueckii and Bacillus subtilis bacterial strains. Reduction of silver ions is an extracellular and rapid process; this information may lead to the development of easy protocols for biosynthesis of the silver nanoparticles. Antibacterial activity of silver nanoparticles is important for the development of effective antibacterial agent against those bacteria who are showing resistance against antibiotic drugs which are available in market.
Egyptian journal of microbiology, 2021
Silver nanoparticles (AgNPs) are broadly applied in numerous industries due to their exclusive physico-chemical and antimicrobial properties. Herein, the biosynthesis of extremely stable silver nanoparticles by the extracellular extract of the novel strain Aspergillus sakultaensis AUMC13885 is investigated for the first time. The physico-chemical characteristics of the synthesized AgNPs were assayed by using UV–vis spectroscopy, transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The UV-vis recorded a maximum absorption band at 405 nm, which matched to the surface plasmon absorbance of silver nanoparticles. Aspergillus sakultaensis AUMC13885 synthesized a uniformly distributed AgNPs of 5-25 nm in size estimated by transmission electron microscopy (TEM). Detection of the proteins binding to the synthesized nanoparticles was conducted by FT-IR analysis. The optimum conditions for AgNPs biosynthesis in this study were 1.0 mM substrate, alkaline p...
Exploitation of Aspergillus niger for fabrication of silver nanoparticles
Extracellular biosynthesis of silver nanoparticles by Aspergillus niger isolated from soil is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-vis spectrum, showing the absorbance at 420 nm (Perkin Elmer Lambda-25). The nanoparticles characterized by Transmission Electron Microscopy exhibited spherical silver nanoparticles with diameter of around 20 nm. Elemental Spectroscopy imaging showed the presence of fungal protein around the silver nanoparticles thereby increasing their stability in the suspension. The silver nanoparticles (10 g/ml) showed remarkable antibacterial activity against gram-positive (Staphylococcus. aureus) and gram-negative (Escherichia coli) bacteria. The reduction of the silver ions might have occurred by a nitrate-dependent reductase enzyme and a shuttle quinone extracellular process. Reduction of silver ions was an extracellular and rapid process; this knowledge may lead to the development of an easy process for biosynthesis of the silver nanoparticles. Potential of fungal-mediated biosynthesis of silver nanoparticles is important for development of effective antibacterial agents showing resistance to drugs available in the market.
2012
Fungi are more versatile in growth and metal tolerance in contrast to bacterial population. Hence they have been used as biofactories for synthesis of nanoparticles. Nanoparticles offer environment friendly solutions for different problems. In present study, biofabrication of silver nanoparticles was carried out from soil isolate Aspergillus niger. The extracellular silver nanoparticles present in fungal filtrate were characterized by various analytical techniques. Upon exposure of the fungal filtrate to silver nitrate, the latter was reduced to silver nanoparticles as indicated by a color change observed by UV-visible spectroscopy. Absorbance was recorded from 300-600 nm and a strong absorbance peak was observed at 430nm. Silver nanoparticles subjected to X-ray diffraction crystallography revealed four peaks at 38, 48 o , 64 o and 72 o at 2 values and correspond to face centered cubic (FCC) nanocrystals. AFM findings showed the silver nanoparticle aggregations. SEM results strongly revealed their surface topography. The optimum experimental conditions were found to be a temperature 37 o C, a pH of 6.0 and a substrate concentration of 2.0mM.
Frontiers in Bioengineering and Biotechnology
In this study, mycelial filtrate of Aspergillus terreus BA6 was used to reduce AgNO3 to form silver nanoparticles (AgNPs). The effect of seven independent variables on the diameter of AgNPs was studied by applying design of experiments (DOE). At optimal conditions, the diameter of AgNPs was reduced by approximately 26.7% compared to the basal culture condition and AgNO3 concentration was found to be the most significant factor affecting the diameter of AgNPs. A. terreus nano-Ag was characterized using UV-visible spectroscopy, transmission electron microscopy, energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zeta potential. The maximum UV absorption was obtained at 420 nm and the microscopic results showed particles with narrow size distribution ranging from 7 to 23 nm. XRD pattern of AgNPs revealed four diffraction peaks of metallic silver and the EDX spectrum showed a strong signal attributed to Ag nano-crystals. AgNPs myco...
Exploitation of Aspergillus niger for Synthesis of Silver Nanoparticles
Journal of Biobased Materials and Bioenergy, 2008
Extracellular biosynthesis of silver nanoparticles by Aspergillus niger isolated from soil is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-vis spectrum, showing the absorbance at 420 nm (Perkin Elmer Lambda-25). The nanoparticles characterized by Transmission Electron Microscopy exhibited spherical silver nanoparticles with diameter of around 20 nm. Elemental Spectroscopy imaging showed the presence of fungal protein around the silver nanoparticles thereby increasing their stability in the suspension. The silver nanoparticles (10 g/ml) showed remarkable antibacterial activity against gram-positive (Staphylococcus. aureus) and gram-negative (Escherichia coli) bacteria. The reduction of the silver ions might have occurred by a nitrate-dependent reductase enzyme and a shuttle quinone extracellular process. Reduction of silver ions was an extracellular and rapid process; this knowledge may lead to the development of an easy process for biosynthesis of the silver nanoparticles. Potential of fungal-mediated biosynthesis of silver nanoparticles is important for development of effective antibacterial agents showing resistance to drugs available in the market.
Biosynthesis of antibacterial silver nano-particles from Aspergillus terreus
World News of Natural Sciences 16 (2018) 117-124, 2018
Bio-synthesis of silver nano-particles using a soil fungi for potential synthesis of metal nanoparticles was examined. The hurried decline of silver (Ag+) ions was monitored using UV-visible spectrophotometer and showed formation of silver nano-particles within 19 minutes. Transmission electron microscopy (TEM) showed that the synthesized silver nano-particles are varied from 16-57 nm and have the varying in shapes like round, rod, uneven. Further the XRD analysis confirms the nano-crystalline phase of silver structure. FTIR examinations confirms the Silver particles. The present examination documents the Biosynthesized silver nano-particles thus created have shown admirable antibacterial activity. The current study, it discloses the increasing both concentration increases the rate of reduction and decreases the particle size.
Nanotechnology using biological principles provides wide range of novel antimicrobials resulting for the overcoming the antibiotic resistant bacterial strains. The usage of fungi for the development of silver based nanoparticles has added advantage that downstream processing and handling the biomass would be much simpler. Various species of Aspergillus included showing very good bactericidal effect which cannot be obtain by using conventional antibiotics. Further the results of color change, pH variations and uv spectra support the nanoparticle synthesis. The wide antimicrobial activity showed the need of rapid synthesis of nanoparticles would be suitable for the development of microbial nanotechnology biosynthesis process for the mass scale production.
Fungal mediated biosynthesis of silver nanoparticles, characterization and antimicrobial activity
Colloids and Surfaces B: Biointerfaces, 2010
Microbial assisted biosynthesis of nanoparticles is a rapidly progressing area of nanobiotechnology. In this paper Aspergillus niger assisted extracellular synthesis of silver nanoparticles is reported. The silver nanoparticles were characterized by UV-vis spectrophotometry, TEM, EDX and FTIR. TEM studies showed the size of the silver nanoparticles to be in the range of 3-30 nm. The probable mechanism for the extracellular synthesis of silver nanoparticles by Aspergillus niger was investigated. The nanoparticles showed antimicrobial activity against fungal and bacterial strains.