Evaluation of newly isolated human vaginal lactobacilli and selection of probiotic candidates (original) (raw)

J. Gen. Appl. Microbiol., 45, 203–212 (1999)

2005

human vagina (Redondo-López et al., 1990) and are routinely found as part of a well-defined “normal” or “indigenous” microflora in the female reproductive tract, first described by Rogosa and Sharpe (1963). More recently, Lachlack et al. (1996) reported the Lactobacillus acidophilus complex, isolated from the vaginal flora in 150 human samples. Furthermore, Reid et al. (1996) identified Lactobacillus species from the vagina of 100 healthy women. There is no consensus on which Lactobacillus species are the main ones in the human vagina, but there is agreement on their number, which is about 10–10 CFU/g of fluid (Redondo-López et al., 1990). The vagina has been increasingly recognized as an ecosystem of which the natural flora helps to avoid the invasion of pathogens. The function of lactobacilli is to maintain an environment that restricts the growth of pathogenic microorganisms (Redondo-López et al., 1990). The female reproductive tract is susceptible to a variety of viral and bacte...

Newly Isolated Lactobacilli strains from Algerian Human Vaginal Microbiota: Lactobacillus fermentum Strains Relevant Probiotic's Candidates

Probiotics and antimicrobial proteins, 2017

Lactobacilli strains are considered as a preventive means for treatment of vaginal infections or post-antibiotic treatment to repopulate the vaginal mucosa. This study aimed at establishing the vaginal lactobacillus profile of Algerian women with different vaginal diseases. Afterwards, lactobacilli isolated from swabs were in vitro characterized for their probiotic hallmarks. This prospective study allowed isolation of 44 Lactobacillus strains and 160 potentially pathogens, among which are Escherichia coli (50 isolates), Staphylococcus sp. (38 isolates), Enterococcus sp. (16 isolates), and Candida sp. (56 isolates). All Lactobacilli strains were characterized for their antagonism, adhesion to polystyrene, and resistance to acidity and bile. Consequently, six Lactobacillus strains (Lb. fermentum 5LB4, 5LB10, 5LB12, Lb. plantarum 5LB2, 5LB11, and Lactobacillus sp. 4LB9) were moderately or weakly adherent, and 35 potentially pathogens exhibited weak to strong adhesion to polystyrene. A...

The probiotic properties and potential of vaginal Lactobacillus spp. isolated from healthy women against some vaginal pathogens

Letters in Applied Microbiology, 2022

During the last decade, probiotic research has progressed considerably and significant advances have been made in the selection and characterization of specific probiotic strains. The most studied probiotics belong to the genus Lactobacillus. In this study, 80 Lactobacillus spp. isolated from healthy women tolerated low pH and were able to grow in the presence of bile salts. RAPD PCR technique resulted in the identification of 38 different types. These isolates were then evaluated based on adhesion capacity, antibiotic susceptibility and tolerance in simulated gastrointestinal tract. Species-specific PCR and detection of bacteriocin-related genes were also surveyed. Among the isolates, five strains—Lacticaseibacillus rhamnosus NO21, Lacticaseibacillus casei NO1, Lactiplantibacillus plantarum NO4, Lactobacillus acidophilus NO7 and Lactobacillus gasseri NO38—presented acceptable antibiotic susceptibility pattern. Further analysis showed antimicrobial activity of Lacticaseibacillus cul...