The influence of low-intensity He-Ne laser on the wound healing in diabetic rats (original) (raw)
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Low-Level Laser Therapy Enhances Wound Healing in Diabetic Rats: A Comparison of Different Lasers
Photomedicine and Laser Surgery, 2007
Objective: The effects of wound healing acceleration on diabetic rats were determined and compared using different laser wavelengths and incident doses. Background Data: Many studies have demonstrated that lowlevel laser therapy (LLLT) can promote the wound healing on non-diabetic animals. Methods: Male Sprague-Dawley rats were used. Streptozotocin (70 mg/kg) was applied for diabetes induction. An oval full-thickness skin wound was created aseptically with a scalpel in 51 diabetic rats and six non-diabetic rats on the shaved back of the animals. The study was performed using 532,633,8L0, and 980 nm diode lasers. Incident doses of 5, 10, 20, and 30 J/cm2 and treatment schedule of 3 times/week were used in the experiments. The area of wound on all rats was measured and plotted on a slope chart. The slope values (mm2/day), the percentage of relative wound healing, and the percentage of wound healing acceleration were computed in the study. Results: Mean slope values were 6.0871 in non-diabetic control and 3.636 in diabetic control rats (p < 0.005). The percentages of wound healing acceleration were 15. 23, 18.06, 19.54, and 20.39 with 532-nm laser, 33.53, 38.44,32.05, and 16.45 with 633-nm laser,15.72,14,94,9.62,and7,76 with 810-nm laser, and 12.80,L6.32, 13.79, and 7.74with 980-nm laser, using incident doses of 5, 10,20, and 30 Jlcmz, respectively. There were significant differences (p < 0.001) in the mean slope value of wound healing on diabetic rats between control groups and treatment groups in 532, 633, 810, and 980 nm lasers. Conclusion: The wound healing on control rats with diabetes was slower than on control rats without diabetes. LLLT at appropriate treatment parameters can enhance the wound healing on diabetic rats. The optimum wavelength was 633 nm, and the optimum incident dose was 10 J/cm2 in our study.
Assessment to the Effects of Low Power Diode Laser on Wound Healing in Diabetic Rats
Objective: Evaluation of the effect of low level laser therapy (LLLT) using diode laser 808 nm on wound healing in diabetic rats as experimental animal model. Back ground data: Delayed wound healing is reported by several medical care units as changing cases. One of the causes for chronic wounds and delayed wound healing is diabetes which sometime associated with suppuration, gangrene and may be ended by amputation. This is encountered in different medical specialities. Methods: 40 male albino rats, each weighed 200-220 gm. Diabetes was chemically induced using streptozotocin, 40 mg/kg, dissolved in citrate buffer solution (pH 4.3) and administered as tail vein injection in all experimental rats groups expect control group . Seven days after streptozotocin injection, blood glucose levels were measured by using a glucometer and test strips. Results: Gross examination showed faster wound closure in the laser exposed groups with minimal scar tissue formation in comparison with non-lase...
Acta Cirurgica Brasileira, 2006
PURPOSE: To study the influence of HeNe laser irradiation on the collagen percentage in surgically-induced skin wounds in rats with and without alloxan-induced diabetes, by morphometric analysis of collagen fibers. METHODS: 48 male Wistar rats were used, divided into groups: laser-treated diabetic (group 1); untreated diabetic (group 2); treated non-diabetic (group 3); and untreated non-diabetic (group 4). For groups 1 and 2, diabetes was induced by intravenous injection of alloxan (2,4,5,6-tetraoxypyrimidine; 5,6-dioxyuracil; Sigma), into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 1 and 3 were treated with HeNe laser (4 J/cm²) for 60 s. One animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin and Masson's trichrome, and morphometrically analyzed using the Imagelab software. Th...
Photomedicine and Laser Surgery, 2006
Objective: The aim of this work was to compare the effect of low-level laser therapy (LLLT) on the wound healing process in nondiabetic and diabetic rats. Background Data: Among the clinical symptoms caused by diabetes mellitus, a delay in wound healing is a potential risk for patients. It is suggested that LLLT can improve wound healing. Methods: The tissue used for this study was extracted from animals suffering from diabetes, which was induced by Streptozotocin ® , and from nondiabetic rats. Animals were assembled into two groups of 25 rats each (treated and control) and further subdivided into two groups: diabetic (n = 15) and nondiabetic (n = 10). A full-thickness skin wound was made on the dorsum area, with a round 8-mm holepunch. The treated group was irradiated by a HeNe laser at 632.8 nm, with the following parameters: 15 mW, exposition time of 17 sec, 0.025 cm 2 irradiated area, and energy density of 10 J/cm 2. Square full-thickness skin samples (18 mm each side, including both injured and noninjured tissues) were obtained at 4, 7, and 15 days after surgery and analyzed by qualitative and quantitative histological methods. Results: Quantitative histopathological analysis confirmed the results of the qualitative analysis through histological microscope slides. When comparing tissue components (inflammatory cells, vessels and fibroblast/area), we found that treated animals had a less intense inflammatory process than controls. Conclusion: Results obtained by both qualitative and quantitative analyses suggested that irradiation of rats with HeNe (632.8 nm), at the tested dose, promoted efficient wound healing in both nondiabetic and diabetic rats as, compared to the control group.
Effect of Laser Dose and Treatment Schedule on Excision Wound Healing in Diabetic Mice
The present study was undertaken to evaluate a He-Ne laser (632.8 nm; 7 mW; 4.02 mW cm )2 ; 15 mm spot size) dose and the treatment schedule on diabetic wound healing in a mouse model. Circular wounds of 15 mm diameter were created on streptozotocin induced diabetic Swiss albino mice, and were uniformly illuminated with the single exposure of various He-Ne laser doses of 1, 2, 3, 4 and 5 J cm )2 respectively. Further, the treatment schedule was also optimized by exposing the wounds with 3 J cm )2 at 0, 24 h, 48 h postwounding. Contraction kinetics, mean area under the curve and the mean healing time of the wounds were computed along with the collagen and the glucosamine levels in the wound ground tissues at various postwounding treatment schedules. Results of this study indicated that the single exposure of 3 J cm )2 laser dose applied immediately after the wounding caused a significant reduction in the mean area under the curve and the mean healing time along with the elevated levels of collagen and glucosamine contents in the tissue compared to the controls. In conclusion, He-Ne laser dose of 3 J cm )2 applied immediately after the wounding has demonstrated optimum wound healing compared to the other doses and treatment schedules.
Influence of ingaalp laser (660nm) on the healing of skin wounds in diabetic rats
Acta Cirurgica Brasileira, 2010
To determine the influence of low-power laser (660 nm) on the collagen percentage and macrophages in skin wounds in diabetic rats. Methods: 30 male Wistar rats were used, distributed in two groups: laser treated diabetic (n= 15); untreated diabetic (n = 15). The diabetes was induced by intravenous injection of alloxan into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 2 were treated with Aluminium Gallium Indium Phosphide-InGaAlP type diode laser (Photon Laser III DMC ®) with a continuous output power of 100 mW and wavelength () of 660 nm (4 J/cm 2) for 24 s. five animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin, Masson's trichrome, and immunohistochemical macrophage. morphometrically analyzed using the Image Pro Plus 4.5 software. The percentages of collagen fibers and macrophages were determined from the samples from the euthanasia animals. Results: The data were treated statistically using analysis of variance (ANOVA) and the Post-hocTukey test. The significance level was set at 0.05 or 5%.Conclusion: The low-power laser (660 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers and macrophages.
Effect of a diode laser on wound healing by using diabetic and nondiabetic mice
The Journal of Foot and Ankle Surgery, 2004
The purpose of this study was to evaluate a 980-nm gallium-aluminum-arsenide diode laser for wound healing. Using genetically diabetic and nondiabetic mice, two 6-mm wounds were created on the back of each mouse by using a punch biopsy. The mice were assigned to 1 of 4 subgroups for laser treatment at different fluence and frequency of treatment: 5 W (18 J/cm 2) every 2 days, 5 W (18 J/cm 2) every 4 days, 10 W (36 J/cm 2) every 2 days, and 10 W (36 J/cm 2) every 4 days. In addition, control mice were used and the wounds were allowed to heal naturally. Wound healing was evaluated on days 5, 12, and 19 by percentage of wounds healed and percent wound closure. A maximum of 5 mice per subgroup were killed at days 7, 14, and 21, and histology was conducted on the wound sites. For diabetic mice receiving 5 W every 2 days, the percentage of wounds healed after 19 days was 100% versus 40% in the control group. Only 20% of wounds in the 10-W diabetic subgroups achieved healing during the same period. For the subgroups whose wounds did not completely heal, all but the 10 W every 2 days subgroup had average closure of Ͼ90%. The 100% closure for the 5 W every 2 days subgroup was significantly greater than the other subgroups. For nondiabetic mice, 100% of the wounds in the 5 W every 4 days and control subgroups were completely healed, whereas 90% of the wounds from the 5 W every 2 days and the 10 W every 4 days subgroups were completely healed. In the latter 2 subgroups, wound closure was 99.4% and 98.8%, respectively. These differences were not significant. The histologic results confirmed these findings. In conclusion, treatment at 18 J/cm 2 shows a beneficial effect on wound healing in diabetic mice and does not have a detrimental effect in nondiabetic mice.
Laser therapy converts diabetic wound healing to normal healing
Photomedicine and Laser Surgery, 2009
Objective: We have determined optimal laser dosimetric parameters in comparison with polychromatic lightemitting diodes (LEDs) that can speed up healing in four animal models: non-diabetic oval full-thickness wounds, diabetic oval full-thickness wounds, non-diabetic burns, and diabetic burns in Sprague-Dawley rats. Materials and Methods: This series of studies used 532-, 633-, 810-, 980-, and 10,600-nm lasers (visible to far infrared) and polychromatic LED clusters (510-872 nm, visible to infrared) as photon sources. Sprague-Dawley rats (n ϭ 893) were used; however, animals that died before and during the experiments from anesthesia accidents and for any other reason were excluded from statistical analysis. Results: The improvements seen (Ͼ10% improvement of impairment) show that phototherapy with the 633-nm laser is quite promising for alleviating diabetic wound and burn healing, and exhibited the best results with 38.5% and 53.4% improvements, respectively. Conclusion: In this induced-diabetes model, wound and burn healing were improved by 40.3% and 45%, respectively, in 633-nm laser dosimetry experiments, and diabetic wound and burn healing was accelerated by phototherapy. This indicates that the healing rate was normalized in the phototherapy-treated diabetic rats. In view of these interesting findings, 633-nm laser therapy given three times per week at 4.71 J/cm 2 per dose for diabetic burns, and three times per week at 2.35 J/cm 2 per dose for diabetic wound healing are recommended as actual doses for human clinical trials, especially after major surgery in those with impaired healing, such as diabetics and the elderly.
Photomedicine and Laser Surgery, 2014
Objective: The aim of present study was to evaluate whether low-level laser therapy (LLLT) can reverse the impaired wound healing process in diabetic rats. Background data: Impaired wound healing in diabetic patients represents a major health problem. Recent studies have indicated that LLLT may improve wound healing in diabetic rats, but the optimal treatment parameters are still unknown. Materials and methods: Male Sprague-Dawley rats (n = 21) were randomly divided into three groups: a healthy control group, a diabetic sham-treated group, and a diabetic LLLT-treated group. Diabetes mellitus was then induced by streptozotocin administration to the two diabetic groups. One 4 cm long full thickness skin incision and one full thickness circular excision (diameter = 4 mm) were performed on the back of each rat. An infrared 810 nm laser with an output of 30 mW, a power density of 30 mW/cm 2 , and a spot size of 1 cm 2 was used to irradiate each wound for 30 sec (daily dose of 0.9 J/cm 2 /wound/day). Results: In diabetic rats, the histology of LLLT-treated excisions revealed a similar healing response to that in nondiabetic controls, with significantly more mature granulation tissue than in the sham-treated diabetic control group. LLLT reduced the loss of tensile strength, and increased the incision wound stiffness significantly compared with sham-irradiated rats, but this did not achieve the same level as in the nondiabetic controls. Conclusions: Our study demonstrates that infrared LLLT can improve wound healing in diabetic rats. Nevertheless, further research needs to be performed to evaluate the exact underlying mechanism and to further optimize LLLT parameters for clinical use.
2014
Background: a review of the literature found no clinical studies in which low level laser therapy (LLLT) and high voltage pulsed current (HVPC) were compared to evaluate their effectiveness in promoting wound healing. Objective: The purpose of this study was compare the effects of LLLT, HVPC and standard wound care (SWC) on the healing of diabetic foot ulcers. Methods: randomized controlled clinical trial where diabetic patients were divided in control group (CG) treated with SWC; HVPC group received HVPC plus SWC; LLLTgroup, treated with LLLT plus SWC. HVPC was applied 45min, 100pps and 100µs. LLLTparameters were 685nm, 30mW,2J/cm 2 applied to the wound edges and 1,5J/cm 2 in the wound bed. All subjects were treated 16 weeks or until the wound closed. The variables were healing, healing proportion, ulcers’s characteristics, protective sensation, nerve conduction studies (NCS) and