The influence of low-intensity He-Ne laser on the wound healing in diabetic rats (original) (raw)

Abstract

The low-level laser irradiation at certain wavelengths is reported to facilitate the healing process of diabetic wounds. Thus, this study carried out to look for the suitable laser parameters that could speed up the healing process. Fourteen healthy male and female rats were used in which a circular wound with a diameter of 2.5 ± 0.2 cm was created on the dorsum in each rat after injected them with alloxan to induced diabetic. They have been divided into two groups: control group (N = 7) and study group (N = 7) to conduct the study. He-Ne laser with a wavelength of 632.8 nm at power density of 4.0 mW/cm 2 was used to irradiate the study group for five times a week until the wound healed (closed) completely, while the control group was kept untreated. The results showed that the laser-treated group healed (wounds were totally closed) faster compared to the control group. In numbers, the laser-treated group healed on average at the 21st day (0.0 ± 0.0 cm) (P ≤ 0.005), whereas the control group healed after 40 days or even 60 days in some cases (sample no. 2). This confirms that laser promotes the tissue repair process of diabetic wounds and reduces the healing period to the half.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (24)

  1. Kajagar BM, Godhi AS, Pandit A, Khatri S (2012) Efficacy of low level laser therapy on wound healing in patients with chronic dia- betic foot ulcers-a randomised control trial. Indian J Surg 74(5): 359-363
  2. Corazza AV, Jorge J, Kurachi C, Bagnato VS (2011) Photobiomodulation on the angiogenesis of skin wounds in rats using different light sources. Photo-Dermatology 25(2):102-106
  3. Sampaio SCPO, de C.Monteiro JS, Cangussú MCT, Santos GMP, dos Santos MAV, dos Santos JN, Pinheiro ALB (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibro- blastic activity during wound healing. Lasers Med Sci 28(3):799- 806
  4. Ma H, Li Y, Chen H, Kang M, Liu TC-Y (2012) Effects of low- intensity laser irradiation on wound healing in diabetic rats. Int J Photoenergy 2012:7pp
  5. P e p l o w P V, C h u n g T-Y, B a x t e r G D ( 2 0 1 0 ) L a s e r photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomed Laser Surg 28(3):291-325
  6. Splinter R, Hooper BA (2006) An introduction to biomedical op- tics. CRC Press
  7. Reddy GK, Stehno-Bittel L, Enwemeka CS (2001) Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair Regen 9(3):248-255
  8. Maiya GA, Kumar P, Rao L (2005) Effect of low intensity helium- neon ( he-ne ) laser irradiation on diabetic wound healing dynamics. Photomed Laser Surg 23(2):187-190
  9. Al-Watban FAH, Zhang XY, Andres BL (2007) Low-level laser therapy enhances wound healing in diabetic rats: a comparison of different lasers. Photomed Laser Surg 25(2):72-77
  10. Saltmarche AE (2008) Low level laser therapy for healing acute and chronic wounds -the extendicare experience. Int Wound J 5(2): 351-360
  11. Houreld N, Abrahamse H (2010) Low-intensity laser irradiation stimulates wound healing in diabetic wounded fibroblast cells. Diabetes Technol Ther 12(12):971-978
  12. G. B. Chiarotto, L. M. G. Neves, M. A. M. Esquisatto, M. E. C. do Amaral, G. M. T. dos Santos, and F. A. S. Mendonça, BEffects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats,^Lasers in Medical Science, 2014.
  13. De Medeiros ML, Araújo-filho I, Maria E, Scarlat W, Queiroz DS, Soares CD (2017) Effect of low-level laser therapy on angiogenesis and matrix metalloproteinase-2 immunoexpression in wound repair. Lasers Med Sci 32:35-43
  14. Uzêda-e-silva VD, Rodriguez TT, Almeida I, Rocha R, Calo F, Xavier A, Nunes J (2016) Laser phototherapy improves early stage of cutaneous wound healing of rats under hyperlipidic diet. Lasers Med Sci 31:1363-1370
  15. Tatmatsu-rocha JC, Ferraresi C, Hamblin MR, Maia FD, Robson N, Driusso P, Parizotto NA (2016) Low-level laser therapy ( 904 nm ) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin. J Photochem Photobiol B Biol 164: 96-102
  16. Fahimipour F, Houshmand B, Alemi P, Asnaashari M, Akhavan M, Akhoundikharanagh F, Emadeddin S, Aminisharifabad M, Setoudehnia A, Mahdian M, Bastami F, Tahriri M (2016) The effect of he -ne and Ga -al -as lasers on the healing of oral mucosa in diabetic mice. J Photochem Photobiol B Biol 159:149-154
  17. AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of vari- ous cultured cells. Lasers Med Sci 27(1):237-249
  18. Borzabadi-Farahani A (2016) Effect of low-level laser irradiation on proliferation of human dental mesenchymal stem cells; a sys- temic review. J Photochem Photobiol B Biol 162:577-582
  19. Santinoni CDS, Oliveira HFF, Batista VEDS, Lemos CAA, Verri FR (2017) Influence of low-level laser therapy on the healing of human bone maxillofacial defects: a systematic review. J Photochem Photobiol B Biol 169:83-89
  20. de la Torre JC (2017) Treating cognitive impairment with transcra- nial low level laser therapy. J Photochem Photobiol B Biol 168: 149-155
  21. Alessi Pissulin CN, Henrique Fernandes AA, Sanchez Orellana AM, Rossi e Silva RC, Michelin Matheus SM (2017) Low-level laser therapy (LLLT) accelerates the sternomastoid muscle regener- ation process after myonecrosis due to bupivacaine. J Photochem Photobiol B Biol 168:30-39
  22. N. Mojarad, A. Janzadeh, M. Yousefifard, and F. Nasirinezhad, BThe role of low level laser therapy on neuropathic pain relief and interleukin-6 expression following spinal cord injury; an experi- mental study,^J. Chem. Neuroanat., vol. in press, 2017
  23. Niemz MH (2013) Laser-tissue interactions: fundamentals and ap- plications. Springer, Berlin Heidelberg
  24. Houreld N, Abrahamse H (2007) In vitro exposure of wounded diabetic fibroblast cells to a helium-neon laser at 5 and 16 J/cm2. Photomed Laser Surg 25(2):78-84