Cell Mechanics and Adhesion- Atomic Force Microscopy-Total Internal Reflection Fluorescence Microscopy(AFM-TIRFM) (original) (raw)

The study investigates the mechanical properties of endothelial cells, particularly focusing on the relationship between mechanical forces acting on the cell surface and the distribution of focal adhesions at the cell's basal membrane. Utilizing Atomic Force Microscopy (AFM) in conjunction with Total Internal Reflection Fluorescence Microscopy (TIRFM), the research reveals insights into the actin cytoskeleton's dynamics under shear stress conditions. Findings indicate that mechanical forces significantly influence the structural organization of the cytoskeleton and the resultant focal contact properties, enhancing the understanding of cell adhesion mechanics.