STUDYING THE OPTIMUM CONDITIONS FOR CELLULASE PRODUCTION FROM WHEAT STRAW AND PAPYRUS STRAW USING TRICHODERMA VIRIDE (original) (raw)
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2010
The purpose of the present work was to study the production and process parameters optimization for the synthesis of cellulase from Trichoderma viride in solid state fermentation (SSF) using an agricultural wheat straw as substrates; as fungal conversion of lignocellulosic biomass for cellulase production is one among the major increasing demand for various biotechnological applications. An optimization of process parameters is a necessary step to get higher yield of product. Several kinetic parameters like pretreatment, extraction solvent, substrate concentration, initial moisture content, pH, incubation temperature and inoculum size were optimized for enhanced production of third most demanded industrially important cellulase. The maximum cellulase enzyme activity 398.10±2.43 μM/mL/min was achieved when proximally analyzed lignocellulosic substrate wheat straw inocubated at 2% HCl as pretreatment tool along with distilled water as extraction solvent, 3% substrate concentration 40%...
APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE
2010
The Solid-state fermentation (SSF) is alternative to submerged fermentation for production antibiotics, single cell protein, enzymes, organic acids, biofuel, etc. However, the advantages of SSF in various processes are found to be greater than in submerged fermentation. This technique not only decreases the cost of the process but also makes product cheaper for consumers. The paper describes experimental applicati;on of SSF on wheat straw for production of mycelia protein and cellulase enzymes by Trichoderma viride. This actual waste from agriculture industry was used as a nourishing base by Trichoderma viride in SSF for cellulase enzyme production. Growth and enzymes production by Trichoderma viride were evaluated on wheat straw and alkali treatment wheat straw (wet processing). The growth of the microorganism (biomass content) shows maximum (123.44 mg/petri dish) on alkali treatment wheat straw compared (96.36 mg/petri dish) on wheat straw during of 240 hours. The results obtained demonstrate that the wheat straw waste from agriculture industry can be used as inexpensive base (carbon source) for industrial production on cellulase enzymes by Trichoderma viride.
Energy Procedia, 2015
Research was conducted to determine the effect of temperature and fermentation time of crude cellulase production by Trichoderma reesei on straw substrate. The research was arranged in a randomized complete factorial design consisting of two factors. The first factor is the temperature of fermentation consists of two levels, 27 o C and 37 o C. The second factor is the time fermentation consists of three levels, namely 5 d, 7 d, and 9 d. The results showed that the temperature and fermentation period significantly affect the observed parameters on crude cellulase production by Trichoderma reesei. The optimal treatment combination to produce crude cellulase with maximum activity is at 27 o C and fermentation time of 9 d with an average value of cellulase activity (filter paperase) and soluble protein respectively 0.041 193 7 unit mL 1 and 0.021 613 mg mL 1 .
Production of cellulase in solid-state fermentation withTrichoderma reesei MCG 80 on wheat straw
Applied Biochemistry and Biotechnology, 1996
It is an accepted fact that ethanol production from lignocellulosic materials is not economical as yet because of the high cost of cellulase production. To reduce the cost of cellulase production, lignocellulosic material (wheat straw [WS]), a comparatively much cheaper substrate, was used instead of costly substrates (pure cellulose or lactose). A pan bioreactor was developed for solid-state fermentation (SSF) that required a small capital investment. High yields of complete cellulase system were obtained compared to that in the liquid-state fermentation (LSF) from WS, when treated with 4.25% NaOH at 121~ for 1 h and mixed with Mandels' medium. A complete cellulase system is defined as one in which the ratio of Bglucosidase activity to filter paper activity in the enzyme solution is close to 1.0. The cellulase system derived from SSF using the pan bioreactor gave more than 85% hydrolysis of delignified WS. The prototype pan bioreactor requires further improvements so that optimum quantity of substrate can be fermented to obtain high yields of complete cellulase system per unit space. The SSF process provides a means for the production of complete cellulase system for the economical bioconversion of renewable biomass into ethanol.
Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml -1 using wheat bran as substrate. The optimal conditions for cellulase production for wheat bran were found to be: initial moisture content-70%, initial medium pH-5.0, temperature-30 o C, moistening agents (MSS) and particle size of substrate (500 µm).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.
Journal of environmental biology / Academy of Environmental Biology, India, 2012
Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml(-1) using wheat bran as substrate. The optimal conditions forcellulase production forwheat bran were found to be: initial moisture content - 70%, initial medium pH-5.0, temperature-30 degrees C, moistening agents (MSS) and particle size of substrate (500 microm).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.
This paper reports the purification and characterization of kinetic parameters of cellulase produced from Trichoderma viride under still culture solid state fermentation technique using cheap and an easily available agricultural waste material, wheat straw as growth supported substrate. Trichoderma viride was cultured in fermentation medium of wheat straw under some previously optimized growth conditions and maximum activity of 398±2.43U/mL obtained after stipulated fermentation time period. Cellulase was purified 2.33 fold with specific activity of 105U/mg in comparison to crude enzyme extract using ammonium sulfate precipitation, dialysis and Sephadex-G-100 column chromatography. The enzyme was shown to have a relative low molecular weight of 58kDa by sodium dodecyl sulphate poly-acrylamide gel electrophoresis. The purified enzyme displayed 6.5 and 55oC as an optimum pH and temperature respectively. Using carboxymethyl cellulose as substrate, the enzyme showed maximum activity (Vmax) of 148U/mL with its corresponding KM value of 68µM. Among activators/inhibitors SDS, EDTA, and Hg2+ showed inhibitory effect on purified cellulase whereas, the enzyme activated by Co2+ and Mn2+ at a concentration of 1mM. The purified cellulase was compatible with four local detergent brands with up to 20 days of shelf life at room temperature suggesting its potential as a detergent additive for improved washing therefore, it is concluded that it may be potentially useful for industrial purposes especially for detergent and laundry industry.
2016
Research on production and the use of cellulase and xylanase by commercial microbial strains is widely reported. However, research on production of cellulase and xylanase by local isolates of Trichoderma harzianum known as potential cellulase producers is still very limited. T. harzianum SNRS3 was used for cellulase and xylanase production from rice straw under solid state fermentation. Our study revealed that unlike Trichoderma sp. that is normally associated with low amounts of β-glucosidase, insufficient to perform an efficient hydrolysis, T. harzianum SNRS3 could be considered as a potential β-glucosidase producer, but not an efficient xylanase producer. As a result of storage of the crude cellulase at room temperature, β-glucosidase activity only decreased to above 80% of its original activity at the end of the 3rd week of storage. The crude cellulase produced by T. harzianum SNRS3 could be industrially applied as the enzyme is still highly active at 60°C and over a wide range ...
Production of Cellulase for Ethanol Fermentation from Pretreated Wheat Straw
Iranian Journal of Science and Technology, Transactions A: Science, 2016
Effect of different media compositions on cellulase production was observed using a strain of Trichoderma viride. Medium with yeast extract as nitrogen source was found the best from the other sources used. Then two modes of fermentation solid state and submerged were compared for the enhanced cellulase production. Better cellulase activity was observed in submerged fermentation than the solid state fermentation. Effect of mash size and agitation was also studied. Small mash size with agitation showed higher cellulase activity than the large size pretreated wheat straw. Bacillus cellulosilyticus was also used for the cellulase production. Trichoderma produced enhanced activities of cellulase enzyme (20.738 ± 0.006 IU). Scarification of pretreated wheat straw released maximum sugar up to 16.1 g/L, after hydrolysis in 48 h using indigenously produced enzyme. The optimum conditions for the saccharification of pretreated wheat straw were 5 and 30°C for pH and temperature, respectively in 48 h. The yield of ethanol was observed 10.4 g/L in saccharified wheat straw based medium.
Enhanced Production of Cellulase by Trichoderma reesei Using Wheat Straw as a Carbon Source
The objective of this study was the screening of a carbon source and microorganism for the enhanced production of cellulose enzyme system. Comparatively, untreated wheat straw (UWS) proved to be the best carbon source and the presence of lignin offered no hindrance to cellulase production by the fungus. Among single cultures, comparatively, T. reesei showed higher specific activity of CMCase and FPase in UWS than T. viride and other strains. The co-culture of T. reesei and T. viride showed better specific CMCase and FPase activity with treated wheat straw (TWS) (3.18 and 2.10) than UWS (1.05 and 1.24). Galactose proved to be a better inducer than glucose or Tween-20 and the specific activity of CMCase (19.21±0.82) and FPase (06.27±0.94) was increased while the production time was decreased from 96 to 72 h. The highest specific cellulase activity was achieved by T. reesei at 30±1°C, pH 6 and 2% four-day-old inoculum with 0.2% (w/v) galactose as the inducer. We achieved the objectives of enhanced production of cellulase using inexpensive lignocellulosic biomass from wheat straw to cut down production costs.