EVALUATION OF ANTIOXIDANT ACTIVITY OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES USING ALOE VERA (original) (raw)

2019, INT. J. BIOL. BIOTECH., 16 (3): 641-653, 2019.

Eco-Friendly green synthesis of nanoparticles is a fast-growing area of research in nanotechnology. This study aims to synthesize Aloe vera mediated silver nanoparticles (AV-AgNPs) and evaluates in-vitro and in-vivo antioxidant potentials. Also examines the phytochemical screening of aqueous Aloe vera leaves extract (AVLE). The AV-AgNPs were characterized by UV–visible spectroscopy and scanning electron microscopy. However, the phytochemical screening of AVLE was performed by standard protocols. In-vitro antioxidant activities were determined through lipid peroxidation inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and reducing power assays. Whereas, in-vivo antioxidant activities were studied in rats (180-220g b.w), received AV-AgNPs (10 mg/kg b.w) via oral gavage for 28 days. After euthanization of rats, blood and kidneys were collected and used for biochemical (urea, creatinine) and antioxidant (MDA, SOD, CAT, and GSH) investigations. Results showed the maximum absorption of AV-AgNPs at 400 nm and 20-24 nm particle size with spherical morphology. Whereas, flavonoid, glycosides, phenols, saponin, tannins, and terpenoids phytochemicals were present in AVLE. Both in-vitro and in-vivo studies exhibited the significant potentials of AV-AgNPs as an antioxidant and free radical scavengers thus it could be proposed for many biomedical and environmental applications such as for drug encapsulation or purification of water.