Characterization and Antimicrobial Investigation of Synthesized Silver Nanoparticles (original) (raw)
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HSOA Journal of Nanotechnology: Nanomedicine & Nanobiotechnology, 2019
Green synthesis is an ecological system for the production of eco-friendly and well characterized metallic nanoparticles using plants. In this research work, the synthesis of silver nanoparticles was from the extract of Annona muricata leaves. The characterization of silver nanoparticles was done with Fourier Transform Infrared spectroscopy (FTIR), UV-vis spectrometry and transmission electron microscope to determine functional groups, shape, size and morphology of synthesized nanoparticle. The anti-antimicrobial potency of the synthesize nanoparticle was investigated via well diffusion method. The UV spectrum of synthesized nanoparticle revealed absorbance at 435.00nm which confirmed the formation of silver nanoparticle. The FTIR analysis shows bands corresponding to -OH, C=O and -NH2 nanoparticle are spherical shape of the functional group. The micrograph obtained from TEM analysis confirmed that the synthesized silver is spherical in shape. The antimicrobial investigation of the silver nanoparticles shows good antibacterial activity due to high zones of inhibition against test bacteria. This work revealed that the synthesized silver nanoparticles possess great antimicrobial potency.
2021
Nanotechnology is the modern research field which deals with design, synthesis and manipulation of particles structure range from 1-100nm in dimension. A nanoparticle is ultra-fine unit with dimension less than 100 nm (1 nm=10-9 meter).Because of their small size they have unique characteristics and their applications in various field such as medicines, engineering and environmental remediation. Nanoparticles can be prepared by various aapproaches like physical, chemical and biological .The biological approach is the emerging approach because it is easier than other methods. In this green synthesis aqueous solution of extract of Azadirathcha indica and AgNo3 were taken for study. The nanoparticles were prepared by using plant extract and silver metal ions. The nanoparticles were characterized by different spectral methods like UV, IR, DLS, SEM, XRD and Zeta analysis.
Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property
Journal of Saudi Chemical Society, 2012
Plants extract from Ocimum tenuiflorum, Solanum tricobatum, Syzygium cumini, Centella asiatica and Citrus sinensis was used for the synthesis of silver nanoparticles (Ag NPs) from silver nitrate solution. Ag NPs were characterized by UV-vis spectrophotometer, X-ray diffractometer (XRD), atomic force microscope (AFM) and scanning electron microscope (SEM). The formation and stability of the reduced silver nanoparticles in the colloidal solution were monitored by UV-vis spectrophotometer analysis. The mean particle diameter of silver nanoparticles was calculated from the XRD pattern according to the line width of the plane, refraction peak using the Scherrer's equation. AFM showed the formation of silver nanoparticle with an average size of 28 nm, 26.5 nm, 65 nm, 22.3 nm and 28.4 nm corresponding to O. tenuiflorum, S. cumini, C. sinensis, S. tricobatum and C. asiatica, respectively. SEM determination of the brown color stable samples showed the formation of silver nanoparticles and well dispersed nanoparticles could be seen in the samples treated with silver nitrate. Antimicrobial activity of the silver bio-nanoparticles was performed by well diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. The highest antimicrobial activity of silver nanoparticles synthesized by S. tricobatum, O. tenuiflorum extracts was found against S. aureus (30 mm) and E. coli (30 mm) respectively. The Ag NPs synthesized in this process has the efficient antimicrobial activity against pathogenic bacteria. Of these, silver nanoparticles are playing a major role in the field of nanotechnology and nanomedicine.
Interest in the biosynthesis of nanoparticles has increased in the last era by researchers. Nanoparticles have several applications in different fields like optoelectronics, magnetic devices, drug delivery, and sensors. Nanoparticle synthesis by green methods is safe for the environment and should be explored and encouraged popularly since various plants' have the high extent to form these nanoparticles. Worldwide, UV spectroscopy, Xray diffraction, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) besides Fourier Transform Infrared Spectroscopy (FTIR) are used in many ways for characterize nanoparticles. The most advantageous use of AgNPs is their great attribution to be used as antimicrobial agents. Finally, concept of AgNPs synthesis is deserved to be the modern technical and medical concern. The current review shows a complete comprehensive and analytical survey of the biosynthesis of AgNPs with a particular focus on their activities as antimicrobials and the possible theories of their effect on the microbial cell and all influenced secondary metabolites.
GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM SELECTED PLANT SPECIES AND THEIR ANTIBACTERIAL EFFECT
Green synthesis of silver nano particles from selected plant species and there anti bacterial activity , 2023
Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowledge from multiple fields covering comparative aspects of pathogen contamination, and unify them in a single place in order to present and address the problem as a whole. Convectional water purification or disinfection techniques, such as chemical treatment, water treatment ozone or chlorine-based components lead to generation of disinfection byproducts. Antimicrobial activities of nano materials have the potential to avoid problems associated with the release of disinfection byproducts. The study reports a versatile, costefficient and eco-friendly protocol for the synthesis of biogenic silver nanoparticles (AgNPs) using the aqueous extracts of selected plant species: that is Bidens pilosa and Plectranthus cyaneus, partial characterization and their anti-bacterial activity. Leaves of the named plant species were collected, washed, cut into pieces and crushed and placed in a 250ml Erlenmeyer flask containing 100 ml distilled water and boiled for 20 minutes. It was the filtered and the filtrate was reacted silver nitrate to synthesize AgNPs. The biosynthesized nanoparticles (AgNPs) were characterized by UV-spectroscopy and Fourier-transform infrared spectroscopy (FTIR). AgNPs were evaluated for their antimicrobial potentials. The UV-Vis spectra showed a complete reduction of Ag+ to Ag0 with Plasmon resonance bands at around 430 nm which is characteristic of AgNPs. FT-IR of the synthesized AgNPs revealed the presence of functional groups: C-O, O-H, =C-H, aromatic C-H bending, C=O, and C=C aromatic attatched to silver nanoparticles. The synthesized AgNPs were observed to possess high activity against Escherichia coli and Staphylococcus aureus respectively. Results of the study revealed that AgNPs exhibited high antibacterial potentials. The antibacterial activity of the biogenic nanoparticles would find applications in the biomedical and environmental fields.
Journal of Genetic Engineering and Biotechnology
Background The Ag-NPs by green synthesis has a notable interest because of their eco-friendliness, economic views, feasibility, and applications in a wide range. Herein, native plants of Jharkhand (Polygonum plebeium, Litsea glutinosa, and Vangueria spinosus) were selected for the current work of Ag-NP synthesis and further antibacterial activity. Green synthesis was performed for Ag-NPs using Silver nitrate solution as precursor and the dried leaf extract performs as a reductant and stabilizer here. Result Visually Ag-NP formation was observed along with a colour change and confirmed by UV-visible spectrophotometry on which an absorbance peak occurs at around 400–450nm. Further characterization was done on DLS, FTIR, FESEM, and XRD. Size around 45–86 nm of synthesized Ag-NPs was predicted through DLS. The synthesized Ag-NPs exhibited significant antibacterial activity against Bacillus subtilis (Gram-positive bacteria) and Salmonella typhi (Gram-negative bacteria). The finest antiba...
Metallic nanoparticles are mostly used in medical fields as it has small size and can easily be used in different applications. In this review, silver nanoparticles and its antimicrobial activities are elaborated for ease of study from different research papers. It has been proven by researchers that nanoparticles have antimicrobial properties. In all metallic nanoparticles, silver nanoparticles have much attention towards antimicrobial properties. Chemicals are used as reducing agents to synthesize nanoparticles, hence it can be used in various biological risks and activities because of its toxic nature also having environmental friendly nature. Biological molecules screened out from plant extracts are used in green synthesis as they are prominent over chemical methods. Plants have vital role in synthesis of metal nanoparticles because plants have biological molecules. This review describes plant diversity which mostly meets with silver nanoparticles having antimicrobial activities.
Journal of Plant Biochemistry and Biotechnology, 2012
The development of phytomediated nanoparticles synthesis is receiving increasing attention due to ease of preparation, less chemical handling, and ecofriendly. In the present study, crystallization of silver ions to nanosized particles by aqueous leaves extract of Cassia javanica through bioreduction process was assessed. Strong plasmon resonance of silver nanoparticles was observed around 435 nm. UV-Vis spectroscopy, transmission electron microscope and Fourier transform infrared spectroscopy were performed to examine the formation of silver nanoparticles (AgNPs). The antibacterial activity of AgNPs was tested against four pathogenic Gram-negative bacteria. AgNPs showed strong antibacterial activity. The strongest activity was recorded on Pseudomonas aeruginosa with 34 mm zone of inhibition followed by Escherichia coli (28 mm), Enterobacter aerogenes (25 mm) and Salmonella typhimurium (23 mm) respectively. The Gram-negative bacteria were highly sensitive to AgNPs, whereas less sensitive to silver nitrate (AgNO 3) and C. javanica leaves extract. The AgNPs were also evaluated for the estimation of total phenolic content. It is concluded that leaves extracts can be used for the synthesis of AgNPs that is environmentally friendly and cost effective. These preparations can be used for various biotechnology and medical applications for controlling pathogenic bacteria. Keywords Cassia javanica leaves Á UV-Vis spectroscopy Á TEM Á FTIR Á Silver nanoparticles (AgNPs) Á Antibacterial activity
Synthesis of Biogenic Silver Nanoparticles From Medicinal Plant And It’s Antibacterial Activity
IOSR Journal of Applied Chemistry, 2016
Biological synthesis of nanoparticles from medicinal plant is gaining importance due to its ecofriendliness. The synthesis of metal nanoparticles is a broad research area due to the potential applications for the development of different novel technologies. In our research work, we have described a cost effective and eco-friendly technique for the green synthesis of silver nanoparticles and evaluated their Antibacterial activity. Synthesis and characterization of silver nanoparticles were carried out by using Crateva religiosa plant bark extract as reducing agent as well as capping agent. The Synthesized nanoparticles were characterized with UV-Visible spectrometry(UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and X-ray Diffraction Spectroscopy(XRD). The antibacterial activity of silver nanoparticles has been Observed.
Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants
Int. J. Chem. Tech. …, 2011
Biologically synthesized silver nanoparticles (SNPs) are being widely using in the field of medicine. Extracellular biosynthesis of silver nanoparticles was carried out by using medicinal plant extracts for the reduction of aqueous silver ions in short period. The silver nanoparticles formation was confirmed by the colour change of plant extracts (SNPs) and further confirmed with the help of UV-Vis spectroscopy. These Phytosynthesized silver nanoparticles were tested for antibacterial and antifungal activities using disc diffusion method. The test cultures are Proteus, Pseudomonas, Klebsiella, Bacillus and E.coli species of bacteria and Aspergillus, Fusarium, Curvularia and Rhizopus species of fungal were used. The microbial property of silver nanoparticles was analyzed by measuring the inhibition zone. The silver nanoparticles synthesized from stem bark extracts of Boswellia and Shorea; and leaf extract of Svensonia. The SNPs synthesized from bark extracts of Boswellia ovalifoliolata and Shorea tumbuggaia showed toxic towards Klebsiella and Aspergillus; and Pseudomonas and Fusarium species respectively. Whereas the growth of Pseudomonas and Rhizopus species were inhibited maximum by the SNPs synthesized from leaf extract of Svensonia hyderobadensis, the results indicate that the silver nanoparticles may have an important advantage over conventional antibiotics.