Screening of Probiotics Efficiency to Inhibit Persistent Biofilm-Related Infections (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: Evaluation of antimicrobial efficacy of probiotic Lactobacillus strains isolated from dairy products. Methods: The study used yogurt sample prepared from raw buffalo milk to isolate Lactobacillus strains by following pour plate technique. All isolated Lactobacillus strains were systematically screened for their probiotic attributes. Here, functional criteria studies for successful probiotics mainly included survival ability of the strains. The capability of the selected Lactobacillus isolates to inhibit the growth of several pathogens was evaluated by the well diffusion method. Based on phenotypic characteristics, biochemical tests and of 16S rDNA sequence analysis, the potent probiotic strain was identified. Further study was emphasized on its optimum parameters required for its growth. Again, its activity was characterized with respect to pH, NaCl and thermal stability along with susceptibility to enzymes. Results: A preliminary subtractive screening based on antimicrobial potency and probiotic properties revealed the maximum effectiveness of Lactobacillus DM 69 among all and it was identified as Lactobacillus plantarum. The inhibitory substance obtained from DM 69 was relatively heat stable and active under acidic conditions. No susceptibility was found in the presence of α-amylase, lipase and lysozyme. Conclusion: Excellent probiotic and an antimicrobial strain was isolated from buffalo milk and can be formulated and developed as a good nutraceutical food supplement.
Important Features of Probiotic Microorganisms in Pharmaceutical and Dairy Products
International Journal of Enteric Pathogens, 2013
Background: Probiotic products are matrices for delivery of beneficial live bacteria to the host. The viable bacteria are being incorporated into dairy products as well as supplements. Objectives: The aim of the present study was evaluation and validation of probiotic contents in commercial products to select the optimum matrix for protection of viability and functionality of probiotic bacteria. Materials and Methods: A total of forty six lactic acid bacteria were isolated from ten pharmaceutical and ten dairy products. Their probiotic properties such as acid, salt and bile tolerance, antibiotic susceptibility tests, adherence to cell line, stability under refrigeration conditions and antagonistic activity against nine bacterial strains were assayed. Results: Results showed that the viable bacterial count of solid products were lower than stated number on their package. No difference was noticed between strains isolated from dairy and non-dairy products regarding antibiotic susceptibility and adherence properties. Pharmaceutical isolates were more potent against pathogens than dairy isolates. Conclusions: In conclusion, dairy products are better matrices for delivering bifidobacteria than non-dairy products. But, probiotic isolates from non-dairy products, showed better properties such as pathogen exclusion than dairy isolates.
Microbiology Research Journal International, 2020
Microbial resistance to antibiotics and biofilm formation ability of food-borne pathogens are major global health challenges. Most milk and milk products (Madara and Nono) could be vehicles for the transmission of multidrug resistant genes among any community. This study was aimed at determining the antibiotic susceptibility patterns and biofilm forming ability of some food-borne pathogens isolated from common dairy products: Madara and Nono in Makurdi metropolis. Two hundred and forty (240) samples comprising of one hundred and twenty (120) each of Madara (fresh raw milk from cow “FRM”)) and Nono (chance fermented cow milk “CFM”) were examined for the presence of pathogens. Antibiogram of bacterial isolates (Staphylococcus aureus, Escherichia coli, Shigella spp., Salmonella spp. and Klebsiella spp.) using the disc diffusion method revealed that susceptibility for Ampicillin (86.9%), Streptomycin (83.9%) and Ciprofloxacin (75.0%). Resistance was shown (26.7%) to Nalidixic acid, a co...
Microorganisms
Over the last 20 years, Lactobacillus species inhabiting the gastrointestinal tract (GIT) have received much attention, and their health-promoting properties are now well-described. Probiotic effects cannot be generalized, and their uses cover a wide range of applications. It is thus important to proceed to an accurate selection and evaluation of probiotic candidates. We evaluate the probiotic potential of six strains of Lactobacillus in different in vitro models representing critical factors of either survival, efficacy, or both. We characterized the strains for their ability to (i) modulate intestinal permeability using transepithelial electrical resistance (TEER), (ii) form biofilms and resist stressful conditions, and (iii) produce beneficial host and/or bacteria metabolites. Our data reveal the specificity of Lactobacillus strains to modulate intestinal permeability depending on the cell type. The six isolates were able to form spatially organized biofilms, and we provide evide...
Probiotic Potential and Safety Properties of Lactobacillus
2013
One hundred and twenty-five acid-resistant presumptive lactobacilli were isolated from Slovak Bryndza cheese and screened for their antimicrobial activity against eight bacterial pathogens using spot agar assay. Out of twenty-six Lactobacillus strains with strong inhibition activity, twenty were identified as Lactobacillus plantarum and six as Lactobacillus fermentum. The most active eleven L. plantarum isolates were further characterized in vitro for some probiotic and safety properties. Only three isolates K10, K21, and ZS07 showed the ability to grow over 50% in the presence of 0.3% bile. Strong deconjugation efficiency was determined for CK06 and K21. The highest-galactosidase activity was shown in isolates ZS11, B01, CK06, and ZS07. Only three of the strains had the ability to produce tyramine: CK06, LM1, and ZS11. Strains K09, K21, ZS11, and ZS15 were susceptible to all tested antibiotics. Analysis of the results confirmed the L. plantarum isolates ZS07 and K21 as the most suitable for probiotic use, due to their desirable probiotic and safety characteristics.
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: The antimicrobial activity and Probiotic properties of Lactobacillus species were evaluated. The antimicrobial compound of potent antimicrobial probiotic Lactobacillus was purified by column chromatography and its nature and stability were determined.Methods: This investigation was performed with few Lactobacillus strains of ATCC and MTCC along with certain strains isolated from different dairy sources. They were evaluated for their probiotics properties (acid tolerance, bile tolerance, bile salt hydrolase activity, cell autoaggregation, cell surface hydrophobicity and haemolytic behaviour). Agar well diffusion method was used to screen their potency to release bioactive compound against several pathogens. This potent antimicrobial compound was purified by chromatography as well as its molecular mass was estimated by following SDS-PAGE. Finally, the stability of the compound was determined against various ranges of temperature and pH.Results: Among all Lactobacillus strai...
Journal of Food Safety, 2013
Three Lactobacilli were isolated from traditional Tunisian fermented food and then characterized for its ability to inhibit Listeria monocytogenes growth. Antagonistic effect of Lactobacillus plantarum on four L. monocytogenes strains was tested in soft artisanal cheese. Anti-biofilm activity of Lactobacillus extracts was also tested. Our results demonstrate that the selected lactic acid bacteria (LAB) extract exhibited a good antibacterial effect against L. monocytogenes ATCC 19115 with low MICs values: 8.33 Ϯ 2.1, 20 Ϯ 1.2 and 23.33 Ϯ 1, respectively. L. plantarum also exhibited a stronger inhibitory effect against L. monocytogenes when grown in cheese. Moreover, a potential anti-biofilm effect of the three LAB extracts with BIC50 values ranging from 5% to 15% for L. monocytogenes ATCC 19115 was demonstrated. Although LAB extracts were able to eradicate significantly a preformed L. monocytogenes biofilm (P < 0.05). Growth inhibition of preformed biofilm was more difficult to achieve. LAB could be used as a bioprotective culture in cheese ripening to prevent Listeria growth. PRACTICAL APPLICATIONS A wide range of lactic acid bacteria (LAB) produce bacteriocins, which were essentially active against the food-borne pathogen Listeria monocytogenes. 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction method was used to assess the anti-biofilm and antilisterial activity of LAB extract. XTT reduction assay is dependent on the microbial respiratory activity. Since XTT is used for quantification of the microbial respiratory activity, it was used to quantify viable mature biofilm and to compare LAB activity against planktonic and biofilm cells.