TP 1 ESTERILIZACION (original) (raw)
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Definición: El prefijo "a" significa negación, falta o ausencia; y "sepsis" infección o contaminación; por lo tanto el término asepsia se define como la ausencia de materia séptica, es decir la falta absoluta de gérmenes.
Esterilización por inmersión de instrumental de cirugía laparoscópica. Estudio comparativo entre glutaraldehído al 2%, agua electrolizada superoxidada con pH neutro y solución electrolizada por selectividad iónica con pH neutro Immersion sterilization of laparoscopic surgery instruments. Comparative study among 2% glutaraldehyde, superoxidized water with neutral pH, and electrolyzed solution by ionic selectivity with neutral pH Abstract Objective: To compare the sterilizing efficacy in vitro of 2% glutaraldehyde (2% GA), of superoxidized electrolyzed water (SEW), and electrolyzed sterilizing solution by ionic activity (ESSIA). Setting: University of Veracruz. Design: Controlled clinical assay. Statistical analysis: Chi square. Material and methods: We prepared contaminating solutions with on E. coli, P. aeruginosa, and methicillin-resistant S. aureus, as well as with C. albicans at concentrations of 1 × 10 8 CFU per milliliter in a sterile saline solution, in which surgical instruments were immersed for 5 min and, thereafter, they were exposed to each of the sterilizing solutions for three different times, 7, 10, and 15 min. Results: After 15 min of immersion in the sterilizing solutions, all cultures assessed in the instruments treated with 2% GA and ESSIA were negative, in contrast to four positive cultures found in the SEWtreated instruments (p < 0.05). Conclusion: Instruments treated with 2% GA and ESSIA comply with sterile requirements.