ASSESSMENT OF GENETIC DIVERSITY IN RICE (ORYZA SATIVA L.) GENOTYPES BASED ON INTER SIMPLE SEQUENCE REPEAT (ISSR) GENETIC MARKER (original) (raw)
Evaluation of gene conservation strategies, phylogenetic relationship and useful traits in genotype, is essential for the study of genetic variation in the marginal population. In this study, a set of 9 rice varieties were analysed using ISSR markers for genetic diversification. ISSR fingerprinting with 5 anchored primers generated a total 52 scorable loci with an average 10.4 amplicons per primer, out of which 32 were polymorphic (61.53%) and 20 were monomorphic (39.47%). The generated amplicons by each primer varied from 8 to 14 in number. The maximum number of polymorphic bands 10 and 7 were obtained in primers ISSR 12 and ISSR1, respectively. Each primer showed polymorphism ranging from 40 percent to 87.5 percent. The average PIC value ranged between 0.86 (ISSR1) to 0.92 (ISSR12). The genotypes possess 0.57 to 0.96 genetic similarity range. UPGMA based clustering analysis using Jaccard's similarity coefficient grouped these genotypes into two major clusters. Cluster I has the highest number of genotypes (7) and Cluster II (2). This led to a demonstration that for genetic diversity studies in rice, ISSR markers can be used effectively.
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