Serodiagnostic studies on bovine leptospirosis in Beni-Suef Governorate (original) (raw)
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Asian Pacific journal of tropical biomedicine, 2014
To evaluate serological findings of bovine leptospirosis which is a zoonotic disease with worldwide distribution caused by Leptospira interrogans. One hundred and sixty seven sera were collected from 9 commercial dairy herds in jiroft suburbs, from July to October 2011. Microscopic agglutination test (MAT) was used to evaluates serological findings of bovine leptospirosis in Jiroft suburb dairy farms, Kerman province, Iran. Antibodies were found by MAT at least against one serovar of Leptospira interrogans in 29 samples (17.36%) among 167 sera at a dilution 1:100 or higher, and Leptospira pomona was the most prevalent serovar. Positive titers against more than one serovar were detected in 6 sera of the positive samples. This study is the first report of leptospirosis in Southeast Iran and showed that Leptospira pomona was the most and Leptospira icterohaemorrhagiae the least prevalent serovars in Southeast Iran.
Polish Journal of Microbiology, 2019
The article presents data on serological studies of 573 sera samples of cattle that were collected from the farms affected by leptospirosis in different regions of Ukraine in the period of 2014–2015. Samples were investigated by the microscopic agglutination test (MAT), which was conducted within eight serological groups of Leptospira and nine serovars: Sejroe (serovars polonica and hardjo), Hebdomadis (serovar kabura), Tarassovi (serovar tarassovi), Pomona (serovar pomona), Grippotyphosa (serovar grippotyphosa), Canicola (serovar canicola), Icterohaemorrhagiae (serovar copenhageni), and Australis (serovar bratislava). The circulation of L. interrogans serovar hardjo among cattle has been observed in all 11 regions of Ukraine investigated within 25.8–60.0% of the leptospirosis-positive serum samples in these regions. Antibodies in the cattle sera against serovar hardjo (serogroup Sejroe) were detected in 139 of the 370 cows reacting positively in MAT. Overall, they were detected in ...
Iranian journal of microbiology, 2011
Leptospirosis is an important zoonotic disease caused by Leptospira interrogans. Leptospirosis leads to economical losses in dairy farm industry. The objective of this study was to evaluate the pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR. Two hundred samples (100 urine and 100 blood) were collected from 100 cows randomly and delivered to the laboratory. Samples were stored at -20 °C. DNA was extracted and purified from the plasma and urine samples and concentrated on diatoms in the presence of guanidine thiocyanate (GuSCN). PCR products were detected and identified as Leptospira by ilumination of the expected size of DNA bands after staining of the agarose gel with ethidium bromide gels. PCR products were purified and sequenced. The results showed that 28% of urine samples and 23% of plasma samples were contaminated. The major serotypes were Icterohaemorrhagiae (50%) and Pomona (37.5%). The urine samples of 17 cows were positive for Lepto...
Seroepidemiological Studies on Leptospirosis among Bovines in an Organized farm
International Journal for Agro Veterinary and Medical Sciences, 2011
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Serologic study on leptospiral infection in sheep in Ahvaz, southwestern Iran
Iranian Journal of Veterinary Research
To investigate the seroprevalence of leptospiral infection in sheep in Ahvaz, southwestern Iran, blood samples were taken from 181 female sheep. Sera were stored at -20°C until use. They were initially screened at serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars pomona, canicola, hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutination test (MAT). The samples were considered positive if ≥ 50% of agglutination of leptospire in a dilution test serum of ≥ l:100 were observed. Sera with positive results were titrated against reacting antigens in serial two-fold dilutions from l:100 to 1:1600. Antibodies against one or more serovars were detected in 27 (14.9%) sera at dilution ≥1:100. Antibodies against more than one serovar were found in 5 (18.5%) positive sera. Among the positive sera, antibodies were most frequent to serovar pomona (43.8%) followed by canicola (21.9%), icterohaemorrhagiae (12.5%), grippotyphosa (9.4%), ...
Iranian Journal of Veterinary Research
Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. In this research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion in industrial dairy farms of Hamedan province, Iran was studied. A total of 80 blood samples were taken from aborted cows in six dairy farms. Sera were tested for antibodies against 6 serovars of Leptospira interrogans (hardjo, pomona, canicola, grippotyphosa, icterohaemorrhagiae and ballum) using microscopic agglutination test (MAT). Antibodies were detected in 18 (22.5%) of the aborted cows, including 17 (21.25%) against L. canicola and 1 (1.25%) against L. pomona. It is concluded that dogs (shepherd and stray) and wild carnivores may have an important role to maintain and transmit the L. canicola infection to the cattle population in this region, therefore, vaccina...
The Journal of Infection in Developing Countries, 2019
Introduction: This study was carried out to determine the seroprevalence and risk factors associated with Leptospira serovars Pomona and Hardjo infection in dairy cows. Methodology: Seroprevalence was determined using ELISA using 160 healthy and 80 recently aborted cows. Risk factor assessment was carried out using a pre-validated questionnaire. Results: The true farm seroprevalence of Leptospira serovars Pomona and Hardjo was 92.3% (95% CI: 66%-98%). In healthy cows, the true and apparent cow seroprevalence of Leptospira serovars Pomona and Hardjo were 26.9 (95% CI: 20–34%), 26.25% (95% CI: 20–33%) and 28.75% (95% CI: 22–36%) and 27.5% (95% CI: 21–35%), respectively. Semi-intensive management system (OR = 11.43; P < 0.01), surface water as a source of drinking water (OR = 1.21; P < 0.03), lack of special wear for visitors (OR = 1.39; P < 0.05), and previous history of abortion (OR = 1.02; P < 0.05) were associated with high rate of seropositivity against Leptospira sero...