DIVERSITY OF ARTHRODERMATACEAE COMMUNITIES THAT CREATE HAVOC TO THE OVERALL HEALTH OF HUMAN AND ANIMALS (original) (raw)
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DNA sequence analysis of keratinophilic fungi isolated from livestock stables in the East of Iran
Current Medical Mycology, 2020
Background and Purpose: Keratinophilic fungi play an important role in the decomposition of keratinous substances in nature. This capacity induces dermatomycosis in both humans and livestock. The soil of livestock stables can be a reservoir of keratinophilic fungi. Therefore, the present study was conducted to isolate and identify keratinophilic fungi in the soil of the livestock stables located in Qayen, South Khorasan Province, Iran. Materials and Methods: This study was conducted on 62 soil samples collected from livestock stables. The samples were cultured by means of hair bait technique (HBT). The identification of the isolates was performed based on their morphological characteristics and then confirmed by polymerase chain reaction and sequencing of the ITS regions of ribosomal DNA. Results: A total of 118 isolates of 7 species from 5 genera were identified. Aphanoascus verrucosu (n=70, 59.36%) was detected as the dominant keratinophilic fungus, followed by Arthroderma quadrif...
Mycopathologia, 2015
Data on the frequency and distribution of keratinophilic fungi in soil of Tunisia are scanty. The present survey aimed to describe the distribution of keratinophilic fungi in soils collected in Tunisia. Keratinophilic fungi were isolated using Vanbreuseghem's hair-baiting technique from 354 soil samples collected in 15 governorates of Tunisia and identified according to their morphology with further DNA and MALDI-TOF analysis when necessary. Keratinophilic fungi were isolated from 46.3 % of the samples from 14 governorates. Chrysosporium keratinophilum was the predominant species (30.5 %) followed by Microsporum gypseum (27.4 %). Other isolated species included C. tropicum (14.0 %), C. indicum (11.0 %), Chaetomium sp. (4.9 %), Arthroderma curreyi, Arthroderma cuniculi (3.7 % each), C. merdarium (3.1 %), Anixiopsis stercoraria, C. parvum, Paecilomyces lilacinus, Auxarthron zuffianum (2.4 % each), Fusarium oxysporum, Aphanoascus verrucosus, Gymnascella dankaliensis (1.2 % each) and 12 other species (0.6 % each). Two to five distinct fungal species were associated with 11.5 % of the positive samples. Keratinophilic fungi were more frequently isolated in rural (54.8 %) than in urban (41.1 %) areas (p = 0.012). The highest (100 %) positive culture rate was noted in soil collected in stables. Keratinophilic fungi are frequent throughout Tunisian territory, particularly in soils with a high organic matter content that should be regarded as humans and animals mycoses reservoir.
Isolation of keratinophilic fungi from soil in Isfahan province, Iran
Journal De Mycologie Medicale, 2012
Objective.-To evaluate the epidemiology of keratophilic fungi in Isfahan province, Iran. Material and methods.-The present research has been conducted on soil samples collected from 16 townships of Isfahan province. For isolate geophilic dermatophytes and keratinophilic fungi, the keratin baiting technique has been applied. Results.-Of 800 soil samples examined, 588 (73.5%) keratinophilic fungi were isolated. The present studied recognized 727 isolates including 16 species of 11 genus, as follows: Chrysosporium keratinophilum (31.4%), C. pannicola (16.9%), C. tropicum (15.4%), Microsporum gypseum (12.4%), Chrysosporium spp. (9.9%), C. indicum (7%), Sepedonium spp. (3.3%), Malbranchia spp. (1%), Trichophyton terrestre (0.8%), T. ajelloi and Paecilomyces lilacinus (0.4%), Engyodontium album and Acremonium spp. (0.3%), Curvularia spp., Fusarium spp. and Ulocladium spp. (0.1%). In this study, E. album was isolated for the first time in this country (Iran). The frequency these keratinophilic fungi are discussed in relation to different agents such as soil pH. Conclusion.-This study contributes to the knowledge of keratophilic fungi in Iran.
Keratinophilic fungi from selected soils of Bahrain
Mycopathologia, 2007
Eighty soil samples were collected from various sites of Bahrain and screened for presence of keratinophilic fungi using hair baiting techniques for isolation. Thirty-six isolates were recovered and identified. The cultures were identified using macroand micromorphological features. Their identification was also confirmed by the BLAST search of sequences of ITS1-5.8S-ITS2 rDNA region against the NCBI/Gene bank data and compared with deposited sequences for confirmation. Eight species of five genera were isolated viz. Aphanoascus fulvuscence (8.75%), Aphanoascus punsolae (20.00%), Chrysosporium indicum (2.50%), Chrysosporium tropicum (2.50%), Chrysosporium zonatum (3.75%), Spiromastix warcupii (1.25%), Microsporum gypseum (3.75%), and Trichophyton mentagrophytes (2.50%). In conclusion, our study indicates that keratinophilic fungi do occur in the various soils of Bahrain. Moreover, the narrow diversity and low density of keratinophilic fungi in the investigated soils is expected and is emblematic to other hot arid environments.
Keratinophilic Fungi Recovered from Muddy Soil in Cairo Vicinities, Egypt
Mycopathologia, 2005
One hundred samples of muddy soil were collected from seven areas in the vicinity of Cairo and screened for the presence of keratinophilic fungi by using hair baiting isolation technique. Forty isolates of keratinophilic fungi were recovered and identified by recognition of their cultures, macro-and micromorphological features. Their physiological and molecular characteristics were studied by determination of their ubiquinone (Coenzyme Q) composition and DNA sequences of (ITS1-5.8S-ITS2) and 18S rRNA region sequences. The Keratinophilic isolates were identified as Chrysosporium carmichaelii, C. queenslandicum, C. zonatum, C. indicum, Aphanoascus mephitalis, and Uncinocarpus reesii. Chrysosporium zonatum was the most prevalent species and represented 42.5% of the total number of isolates. Each of C. carmichaelii and C. queenslandicum were equal in their prevalence and represented 15%. C. indicum comes next constituting 12.5%; followed by Uncinocarpus reesii which represented 10%. The least prevalent species in our study was Aphanoascus mephitalis, which was represented only 5% of the total keratinophilic isolates.
Distribution of Keratinophilic Fungi in Desert Soil of Kuwait
Mycoses, 2009
Forty soil samples from different desert localities in Kuwait were surveyed for keratinophilic and geophilic dermatophytic fungi. Sixteen species classified in 9 genera were recovered on wool baits. Chrysosporium species were the most common fungi: Chr. tropicum (60%), Chr. pannicola (32.5 %), Chr. queenslandicum (27.5 %), Chr. keratinophilum (25 %), Chr. indicum (20%) and the Chrysosporium anamorph of Arthroderma cuniculi. The second dominant genus was Malbranchea (27 %) represented by two species; followed by Myceliophthora (10%). A number of cleistothe-cia1 keratinophilic species were also reported in restricted areas. Cteizomycesserratus Eidam and its Myceliophthora anamorph were new records to Kuwait. The frequency and distribution of these fungi have been discussed in relation to ecological conditions of desert soils. Zusammenfassung : Vierzig Erdproben von verschiedenen Wiistenboden in Kuwait wurden auf keratinophile und geophile Dermatophyten untersucht. Siebzig Arten wurden in 9 Gattungen klassifiziert. Chry-sosporiumArten herrschten vor.( Chr. tropicum (60%), Chr. pannicola (32,5%), Chr. queenslandicum (27.5 %), Chr. keratinophilum(25 %), Chr. indicum(20%), und Chrysosporium spec. Anomorph von Arthroderma cuniculi)
Journal of Animal and Plant Sciences, 2012
In order to determine the incidence of dermatophytes and related keratinophilic fungi present in Riyadh soil, eighty soil samples were collected .These samples were screened using hair baiting techniques for isolation. Out of a total 80 samples, 69 (86%) were positive for growth of keratinophilic fungi. Eleven genera and 19 species were isolated and identified, of which Chrysosporium indicum (33.75%) was the most predominant species isolated followed by C.tropicum (26.25%), Aspergillus flavus (17.50%), Microsporum gypseum (13.75%) and Trichophyton terrestre (11.25%). Garden soils, followed by playground soils were found to be the most suitable for fungal growth. Some of the other fungi isolated were C.zonatum , Alternaria alternata, Aspergillus niger, Aspergillus terreus, Fusarium moniliforme,F.solani ,Aphanoascus fulvescens etc. To our knowledge, this appears to be the first report concerning the isolation of keratinolytic fungi M. canis and Trichophyton terrestre from soils of Riy...
The occurrence of keratinophilic fungi in selected soils of Ladakh (India)
Natural Science, 2010
138 soil samples were collected from various locations in Ladakh, a cold desert in the Himalayan region, India and the samples were screened for the presence of keratinophilic fungi using the hair baiting techniques. 58 isolates were recovered and identified. The cultures were identified based on their macro-and micro-morphological features. A total of six genera and fourteen species were isolated namely Amauroascus kuehnii (0.72%), Aphanoascus keratinophilus (4.34%), Aphanoascus terreus (2.17%), Auxarthron alboluteum (0.72%), Auxarthron conjugatum (0.72%), Chrysosporium articulatum (0.72%), Chrysosporium mephiticum (0.72%), Chrysosporium minutisporosum (2.17%), Chrysosporium siglerae (0.72%), Chrysosporium sp. (1.44%), Chrysosporium tropicum (15.94 %), Chrysosporium submersum (3.62%), Chrysosporium state of Ctenomyces serratus (6.52%) and Geomyces pannorum (1.45%). The present study shows that keratinophilic fungi exist in the cold desert of Ladakh.
BioMed Research International, 2013
Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz.Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced.Results. 411 isolates from 22 genera were identified.Fusarium(23.8%),Chrysosporium(13.13%),Acremonium(12.65%),Penicillium(12.39%),Microsporum gypseum(1.94%),Bionectria ochroleuca(1.21%),Bipolaris spicifera(1.21%),Scedosporium apiospermum(0.82%),Phialophora reptans(0.82%),Cephalosporium curtipes(0.49%),Scedosporium dehoogii(0.24%),Ochroconis constricta(0.24%),Nectria mauritiicola(0.49%),Chaetomium(0.49%),Scopulariopsis(0.24%),Malbranch...