Occurrence of oxytetracycline residues in milk samples from Palakkad, Kerala, India (original) (raw)
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Screening of oxytetracycline residue in cattle milk collected from Jaipur, Rajasthan
Journal of entomology and zoology studies, 2019
In India, antibiotics are being used in large quantities for decades, until the existence of these drugs in the milk has received a little concern. The prolonged or inappropriate usages of antibiotics are leading to residues, which pose the risk of human health hazards and are also interfering with the processing of the milk and milk products. The separation of Oxytetracycline antibiotic was achieved on an Acclaim C18 analytical column (150 mm x 4.6 mm x 5 μm). The mobile phase was a mixture of.01M oxalic acid, acetonitrile and methanol (70:20:10 v/v) at a flow rate of 1ml/min at 30C temperature. Milk samples were collected from different places of Jaipur. Antibiotic residues were detected in most of the samples. It was found that there was an increase in the residual level of milk up to three days of treatment after that the concentration of the antibiotic residue starts declining.
STUDY ON THE PREVALENCE OF ANTIBIOTIC RESIDUES IN MILK SAMPLES COLLECTED FROM KATHMANDU VALLEY
ABSTRACT Veterinary drug residues in milk, meat and eggs are growing concern on food safety issues globally due to their involvement in human health hazards. This study was carried out to examine seven types of Tetracycline, Chloramphenicol and Streptomycin by ELISA test in milk samples sold at different places of Kathmandu valley. A total of 140 milk samples were collected from three different sources (organized dairies, cottage dairies and individual farmers).
Saudi Journal of Medical and Pharmaceutical Science, 2023
This study was conducted to evaluate the presence and concentration of Oxytetracycline and Penicillin G residues in raw cow and Fermented milk consumed in Maiduguri, Northeastern Nigeria. A total of 172 (86 raw and 86 fermented) milk samples were randomly collected from different milk selling points in Maiduguri. A Spectrophotometer ultraviolet visible spectrum machine was used to detect and quantify the oxytetracycline and penicillin G residues in the milk samples. All samples (raw and fermented milk) tested positive for oxytetracycline and penicillin G residues with a mean residual concentration of 36 µg/L and 20 µg/L in raw and fermented milk respectively for oxytetracycline while the mean residue concentration of penicillin G is 649 µg/L and 397 µg/L in raw and fermented milk respectively. However, the oxytetracycline residues detected were all below the Maximum Residue Level (MRL) standards of 100 µg/L, while the penicillin G residue detected were all above the maximum residue standard of 5 µg/L. The maximum and minimum residue for oxytetracycline detected in raw milk was 79 and 10 (µg/L) respectively while the maximum and minimum residue detected in fermented milk was 42 and 9 (µg/L) respectively. The maximum and minimum residue detected for penicillin G residue in raw milk was 1993 and 767 (µg/L) respectively while the maximum and minimum residue detected in fermented milk was 288 and 164 (µg/L). This study revealed the presence of both oxytetracycline and penicillin G residues in raw and fermented milk consumed in Maiduguri. However, oxytetracycline residues detected in this study were found to be within the acceptable limits, while the penicillin G residues were highly above the MRL standard set by CODEX.
Detection of antibiotic residues in raw cow milk in Thrissur, India
Antibiotic residues have been recognized as an important threat to food safety ever since they created problems in dairy industry in the form of starter failure. The antibiotic residues in milk are of immense public health significance as they cause adverse health effects in humans. In the present study, the raw milk samples collected from farmer households in Thrissur district were analysed for the presence of antibiotic residues. The samples were subjected to screening by microbial inhibition assay (MIA). The positive samples in MIA were analysed by charm ROSA assay to detect the presence of tetracycline, ß-lactams and enrofloxacin residues at violative levels. The results showed the occurrence of antibiotic residues in 8.48 per cent of samples. Tetracycline, ß-lactams and enrofloxacin residues were detected at violative levels in three, four and three samples respectively. The presence of antibiotic residues clearly indicates misuse of antibiotics and insufficient withdrawal period followed.
Iranian Journal of Veterinary Medicine, 2014
BACKGROUND: Tetracyclines (TCs) are broad-spectrumantibiotics that are widely used in veterinary medicine. Thepresence of TCs residues in milk is a public health concern allover the world. OBJECTIVES:This study aimed to determine TCsresiduals in pasteurized milk marketed by some dairy companiesin Tehran from April 2011 to March 2012. METHODS: 432pasteurized milk samples were purchased from supermarketssupplying the milk products of 12 major dairy companies inTehran (3 samples from each company every month), and theywere stored at -20 0C until analysis. Oxytetracycline (OTC) andTetracycline (TC) residues in each sample were extracted by aliquid - liquid phase procedure and quantitated using a highperformance liquid chromatographic (HPLC) method. Chromatographicconditions included a mobile phase as oxalic acidbuffer- acetonitril (80: 20) with a flow rate of 1mL/min and UVdetectionat 355 nm. RESULTS: TCs residuals in most milksamples were lower than 100 ppb, maximum residue level(MRL);...
Determination and Human Health Risk Assessment of Antibiotic Residue in Cow Milk in Bangladesh
2020
Consumption of milk contaminated with antibiotic residues above the maximum residue limit (MRL) causes direct toxicity to humans and the development of superbugs that leads to the failure of antibiotic therapy and threatens human life. Besides, long-duration exposure might alter the nature of gut microflora results in the enhancement of many diseases. Therefore, we examined 300 raw and processed packet milk samples using thin layer chromatography (TLC) and ultra high performance liquid chromatography (UHPLC) method against five veterinary antibiotics and assessed the risk for consumers in Chattogram, Bangladesh. Risk analysis was calculated by using hazard quotient on the basis of 165 ml per capita milk consumption. We found a total of 7% prevalence of antibiotic residues in raw milk samples which were higher (8%) in individual samples than the pooled samples (4%). However, we did not find any processed packet milk samples as positive. The mean concentration of oxytetracycline resid...
2014
Summary The objective of this study was to determine the oxytetracycline residues in cow’s milk collected in farms of Tetovo in Macedonia. The cow’s milk samples produced in this area are controlled applying qualitative analytical tests for oxitetracycline residues in 262 milk samples through specific ELISA test. After this control positive milk samples were kept in freezing conditions to be analyzed by high performance liquid chromatography (HPLC) method in order to perform qualitative evaluation of oxytetracycline. The quantitative control with ELISA confirmed that 5.3% (14/262) of milk samples were positive for oxytetracycline residues. From analytical check performed by HPLC was confirmed that 2.3% (6/262) of total milk samples had different values oxitetercycline residues. Referring to MRL for oxitetracycline in milk only 1.6% (4/262) of analyzed samples has been confirmed with values higher than this limit. The quantity values of oxitetracycline calculated after analytical che...
2014
A cross-sectional study was conducted between October 2007 and May 2008 to detect and determine oxytetracycline and penicillin G residue levels in bulk milk of cows in Nazareth dairy farms. A total of 400 bulk milk samples were randomly collected. All samples were qualitatively screened for antibiotic residues by Delvotest SP assay. Questionnaire survey was carried out by personal interviews with some of the dairy farm owners in Delvotest positive farms (cases) and Delvotest negative farms (controls) to identify various risk factors for the occurrence of antibiotic residue in milk. Farm management practices, disease conditions and treatment factors have been identified as the main risk factors. Concentration of Oxytetracycline and Penicillin G in the positive samples was determined by High Performance Liquid Chromatography. Out of 400 samples analyzed for antibiotic residue, 48 (12 %) milk samples were positive for antibiotic residues. The mean residue level of oxytetracycline was 1...
Screening of antibiotic residues in fresh milk of Kathmandu Valley, Nepal
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 2017
The prevalence of two groups of antibiotics; namely penicillin and sulfonamides was studied in fresh milk available in Kathmandu Valley of Nepal. The milk samples (n = 140) were collected from three different sources; individual farmers, cottage dairies and organized dairies of Kathmandu valley. Qualitative and semi-quantitative analysis with rapid screening kits revealed that 23% samples were positive for antibiotic residues in the fresh milk for penicillin and sulfonamide groups (1-256 µg/kg). High performance liquid chromatography (HPLC) analyses detected 81% samples positive for amoxicillin (68-802 µg/kg), 41% for sulfadimethoxine (31-69 µg/kg), 27% for penicillin G (13-353 µg/kg), and 12% for ampicillin (0.5-92 µg/kg). Due to the precision and accuracy of liquid chromatography method, it detected more positive samples and consequently presented higher prevalence than the rapid screening kits. The antibiotic residues were found above the maximum residue limits that presented ser...
2014
The evaluation of oxatetracycline residues in cow’s milk in cattle farms in Tetovo, Macedonia was carried out from 2013 to 2014. The control covered 25 milking cows’ farms analyzing cow’s milk samples for level of oxyteteracycline. This study was completed performing analytical control for detection of oxatetracycline residue in 135 fresh milk samples using qualitative ELISA test. The positive samples confirmed by ELISA were kept in refrigerated condition and later are analyzed by high performance liquid chromatography (HPLC). The laboratory detection by HPLC made quantitative evaluation of oxyteteracycline residues in milk samples. Analytical control carried out by ELISA test confirmed presence of oxyteteracycline residues in 4.4% (6/135) of raw milk samples. Further analytical check with HPLC evaluated all positive milk samples confirming different values of oxyteteracycline per liter milk: 60ug/l, 90ug/l, 220ug/l, 260ug/l, 430ug/l, 1340ug/l (ppb). The found values of oxatetracycl...