Siderophore Production by Rhizosphere Inhabiting Bacteria and Fungi (original) (raw)
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Selection and Characterization of Rhizosphere Fungi Producing Siderophore
International Journal of Current Microbiology and Applied Sciences, 2019
is less soluble. As a result, they are difficult to be used by organisms. They are different from some microorganisms like bacteria (Arora, 2017; Patil 2014, Chakraborty, 2014), yeast (Ghosh, 2015) and fungi (Ghosh, 2017; Usha, 2013) that can secrete secondary metabolite siderophore with less iron compound (Hussein, 2019; Prema, 2013). Some fungi producing siderophore are Trichoderma harzianum, T. viride, T. asperellum (Ghosh, 2017), Aspergillusniger and A. flavus (Usha, 2013).
2017
Four different isolates were derived from the fungal and bacterial origin from the soil sample taken from rhizosphere for the detection of siderophores production. Biochemical characters were studied and the isolates were identified. Isolated colonies were confirmed as E. coli, P. fluorescens, A. flavus, and Rhizopus sp. The high amount of siderophores production was observed by Pseudomonas fluorescens from bacterial isolates, and from fungal isolates Aspergillus flavus showed more production and produces hydroxamate type of siderophores in high production compared to that of the catecholate. Thus, we can conclude that rhizosphere soil organisms which produce siderophores can be used to improve the plant growth.
Journal of Microbiological Methods, 1999
A well-known and widely used method for detection of siderophore production by microorganisms in solid medium is the universal chrome azurol S (CAS)-agar plate assay. However, the high toxicity of CAS-blue agar medium caused by the presence of a detergent impedes its utilization with many varieties of fungi and Gram-positive bacteria. To solve this problem, a modification of the CAS-agar plate assay was made by incorporating the CAS-blue dye in a medium with no contact with the microorganisms tested. Half of each plate used in our experiments was filled with the most appropriate culture medium for each type of microorganism and the other half with CAS-blue agar. This modification allowed us to study several strains of fungi (basidiomycetes, deuteromycetes, ascomycetes and zygomycetes) and bacteria (Gram positive and negative), some of them appearing for the first time in the literature. All the microorganisms grew properly and reacted in different manners to the CAS assay. Some strains of wood-decaying basidiomycetes (mainly white-rot fungi) and Aspergillus species produced the fastest color-change reactions in the CAS-blue agar. This modified method could facilitate optimization of culture conditions, since both CAS-blue agar and growth medium were prepared and added in the Petri plate separately.
IJRASET, 2021
Siderophores are low molecular weight, Fe ion specific chelating agents which have been elaborated by microorganisms growing under low Fe stress. The present work aimed to detect and study the biodiversity of the Parnera hill forest and identify suitable different microorganisms which produce alternative, environment-friendly iron chelating agent i.e. Siderophores. To fulfill these objectives, the soil samples are collected from Parnera hill forests from different geographical locations during variable times and seasons. Isolated microorganisms were detected and identified for Siderophore production by CAS agar assay, level of Siderophore production is determined using various culture media. Also detailed study was carried out to identify types of siderophore produced using Arnow's and Tetrazolium test, Spectrophotometric assay, FeCl 3 test, Vogel test. The qualitative analysis of culture filtrates revealed the presence of Hydroxamate nature of siderophores for isolates S1
Characterization and siderophores production of Rhizobium spp. isolated from wild legumes
International journal of computational and experimental science and engineering, 2020
Legume plants are very significant not only ecologically but also agriculturally because they are responsible for major change of nitrogen from atmospheric N2 to ammonia. In this study, total 56 isolates of Rhizobium spp. which were previously isolated from wild legumes plant (Melilotus officinalis, Medicago sativa and Vicia cracca) existing in the central and the disricts of Kırşehir province (Kaman, Mucur, Akpınar, Akçakent, Çiçekdağı, Boztepe). In order to characterize the isolates; YMA containing Bromothymol blue, Congo red, Gram stain reaction , movement, catalase and oxidase tests were evaluated. In addition, isolates of Rhizobium spp. (wild type) were screened for their ability to produce siderophores and it was determined that 50 of 56 isolates in total can produce siderophore.
Isolation and application of siderophore producing bacteria
2017
Siderophores are low molecular weight metal chelating agents which are produced by plants and microorganisms in Felimiting conditions. In this study bacteria were isolated from different soil sample and were characterized for PGPR traits. Biochemical characters were studied and the isolates were identified. Influence of different environmental factors, such as carbon source, nitrogen source, pH, temperature and salt stress, on siderophore production was studied. Isolates were further used to increase the fertility of the soil to help the plantlets grow well and increase the Fe content of the plantlets. It was found that different siderophore producing bacteria produced siderophore at different time and with different percentage. At different environmental condition the amount of siderophore produced was different. They also increased the iron concentration in plantlets grown in different soil samples.
2011
Out of 74 isolates, only 29 (39.2%) diazotrophic rhizobacterial isolates showed siderophore production by CAS (chrome azurol S) plate assay. Optimum siderophore production was recorded after 72 h of growth with 2 mM of FeCl 3 at 30 o C temperature in shaking condition in JNFbliquid medium. The amount of siderophore produced by these isolates ranged between 1.45 to 22.22 µg/mg dry weights. 15 (51.7%) isolates were catecholate and 14 (48.3%) were hydroxamate type. TLC analysis of catecholate type siderophores revealed that only 13.8% was exactly similar to 2, 3dihydroxybenzoic acid (DHBA) whereas 86.2 % showed more than one type of siderophores or other catecholate siderophore with varying molecular weights. The siderophore production under 5.0% NaCl stress by Agrobacterium sp. strain BN-2A is the first report.
Microbial siderophore – A boon to agricultural sciences
Biological Control, 2020
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