Isolation and Characterization of Crude Oil Degrading Bacteria in Association with Microalgae in Saver Pit from Egbaoma Flow Station, Niger Delta, Nigeria (original) (raw)
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Crude Oil Biodegradation Potential of Bacterial Strains Isolated from Mangrove Ecosystem
Futo Journal Series (FUTOJNLS), 2016
Crude oil biodegradation potential of rhizosphere bacterial strains isolated from mangrove ecosystem in the Niger Delta of Nigeria was investigated. Total microbial load of rhizosphere soil sample indicated 2.06 x 10 6 , 5.1 x 10 3 and 1.61 x 10 5 cfu/g for total aerobic heterotrophic bacterial, total fungal and total hydrocarbon utilizing bacterial counts, respectively. Among 18 oil-degrading bacterial strains, Bacillus sp. RS12, Pseudomonas sp. RS16, Pseudomonas sp. RS23, and Micrococcus sp. RS38 were selected for the study based on their efficiency of crude oil utilization in both BHA-crude oil and NA-crude oil plates. Results showed that the test organisms could utilize crude oil, diesel, petrol, kerosene and engine oil as carbon sources. None of the organisms utilized hexane and xylene but moderate growth was observed in phenol. The test isolates exhibited wide range of adherence to crude oil with values of 46.7 to 65.8%. The adhesion to crude oil was in the following order: Pseudomonas sp. RS23 (58.40%) > Pseudomonas sp. RS16 (55.51%) > Bacillus sp.RS12 (50.70%) > Micrococcus sp. RS38 (48.82%). However, their ability to utilize crude oil varied both in rates of utilization and in growth profile with 45 % of crude oil degraded and 0.30 g/l biomass formed by Pseudomonas sp.RS23. The least crude oil degradation potential was 33 % with 0.25 g/l of biomass formed was observed in Micrococcus sp. RS38. The high crude oil utilization of the test isolates implies that bacteria isolated from contaminated ecosystem are excellent crude oil degraders and can be harnessed for bioremediation purposes.
Biodegradation of Crude Petroleum Oil by Indigenous and Isolated Bacterial Strains
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Newly isolated bacterial strains (i.e. Pseudomonas, Bacillus and Micrococcus species) were able to degrade crude oil. In unfertilized soil 30% crude oil was degraded very slowly while 80% of oil was degraded in fertilized soil in the same time duration (i.e. 90 days). During the next 90 days, 60% and 90% of crude oil was degraded in unfertilized and fertilized soil, respectively. Lower fractions of the crude oil were degraded completely and quickly as compared to higher fractions. Addition of nutrients to the soil resulted in the increase in rate of biodegradation of crude oil. A consortium of isolated indigenous cultures removed 80% of the crude oil in 20 days of incubation while the oil contaminated soil seeded with a pure Pseudomonas sp. IES-Ps-1 degraded <5 %.
Biodegradation Potential of Bacteria Isolated from Crude Oil Polluted Site in South South, Nigeria
Aims: This study aimed to determine the biodegradative potential of crude oil by Chryseobacterium sp. and Lysinibacillus fusiformis isolated from Crude Oil site in Gokana LGA, of Rivers State. Study Design: Research was designed to evaluate the speed of degradation of crude oil by bacterial species over a period of 28 days. Place and Duration of Study: Study was carried out in the Microbiology Laboratory of Rivers State University from March to April 2018. Methodology: Four experimental set ups were used: mineral salt broth with crude oil only (Control), Chryseobacterium sp. in mineral salt culture with crude oil (Set-up 1), Lysinibacillus fusiformis in mineral salt culture with crude oil (Set-up 2) and Chryseobacterium sp.+Lysinibacillus fusiformis in mineral salt culture with crude oil (Set-up 3). Analysis of pH and Total Viable Counts was carried out at weekly intervals while Total Petroleum Hydrocarbon was carried out at biweekly intervals. Results: Results showed a steady decline in pH from almost neutral (6.1) to acidic with mixed culture set up having the lowest value of 4.19 < Lysinibacillus fusiformis set up of 4.22 < Chryseobacterium sp. set up of 4.34. Total Petroleum Hydrocarbon followed the same trend of Original Research Article
Research Article Bacterial Biodegradation of Crude Oil Using Local Isolates
An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22 ∘ C, the mixed bacterial consortium degraded a maximum of 88.5% of Egyptian crude oil after 28 days of incubation. This was followed by 77.8% by Pseudomonas aeruginosa, 76.7% by Bacillus subtilis, and 74.3% by Acinetobacter lwoffi. The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities when they are used together in mixed consortium.
Bio-Research
The crude oil degradation potential of bacterial isolates from three contaminated sites in Nigeria were investigated. Seven bacterial isolates namely Pseudomonas aeruginosa strain W15, Pseudomonas aeruginosa strain N3R, Serratia marcescens strain N4, Providencia vermicola strain W8, Serratia marcescens strain W13, Pseudomonas aeruginosa strain W11 and Pseudomonas protegens strain P7 were isolated and identified using molecular methods. Isolates N4, N3 and W13 showed higher % total petroleum hydrocarbon (TPH) degradation of 79.26%, 78.96% and 78.69% respectively than W15, P7, W8 and W11 with % TPH degradation of 68.96%, 62.14%, 59.75% and 59.00% respectively. W13 showed the fastest degradation rate with 78.72% within the first 14 days of incubation; however, after the 14th day, there was no progressive change in % TPH. W11 showed degradation of wider range of hydrocarbon components originally in the crude oil as well as the complete degradation of most intermediates formed. The isola...
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium
Brazilian Journal of Microbiology, 2015
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.
Bacterial biodegradation of crude oil using local isolates
An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22 ∘ C, the mixed bacterial consortium degraded a maximum of 88.5% of Egyptian crude oil after 28 days of incubation. This was followed by 77.8% by Pseudomonas aeruginosa, 76.7% by Bacillus subtilis, and 74.3% by Acinetobacter lwoffi. The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities when they are used together in mixed consortium.
The ability of three bacterial isolates (Bacillus spp, Micrococcus spp and Proteus spp.) and some fungal species (Penicillin spp., Aspergillus spp. and Rhizopus spp.) isolated from two rivers and refinery effluent to degrade two Nigerian Crude oils was studied. The results showed changes in pH, optical density and total viable count for the bacterial isolates after a 17-day period. There was an increase in biomass for the fungal isolates after a 35-day period. It was observed that these organisms were able to utilize and degrade the crude oil constituents, with bacterial isolates showing increase in cell number and optical density as pH decreases. Single cultures were observed to be better crude oil degraders than the mixed cultures (bacteria or fungi). It was also observed that oil degraders could be isolated from a non-oil polluted environment, although those from oil-polluted environments have higher degradation potentials.
International Journal of Environmental Research, 2019
Petroleum hydrocarbons are highly toxic to plants, animals, and humans and are carcinogenic effects to plants, animals, and humans. The present study is focused to enhance the degradation of petroleum hydrocarbons using potential indigenous bacterial isolates. Bacteria were isolated from different petroleum oil-contaminated sites and characterized. Bacterial growth was evaluated under different physico-chemical parameters. The petroleum hydrocarbon degradation potentiality was assessed using GC-MS analysis. Forty-nine bacterial isolates were screened; only three isolates (ALK-14, ALK-16, and ALK-23) have good potential to degrade petroleum hydrocarbons and were identified as Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23, respectively, on the basis of morphology, biochemical characterization, and 16S rRNA sequencing. Optimum growth of Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 occurred at pH 7, 6, and 6, respectively. Petroleum oil concentration found suitable for growth of selected bacterial isolates Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 were 4%, 8%, and 4% (v/v), respectively. Among different nitrogen sources, ammonium nitrate was found suitable source for the maximum growth of Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16 at the concentration of 0.15 and 0.2%, respectively, and sodium nitrate for Enterobacter species ALK-23 at the concentration of 0.2%. All bacterial isolates showed maximum growth at 30 °C temperature. Synergistic effect on the growth of bacterial isolates under favourable conditions increased up to 11-28%. GC-MS analysis indicated that the hydrocarbon compounds in the range of C 20-C 44 were present in petrol. Maximum hydrocarbon degradation by Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 was 11.65%, 8.11%, and 5.59%, respectively. The degradation of docosane 11-decyl, hexatriacontane, and eicosane by Alcaligenes species ALK-14 was 100%, 60%, and 48.8%, respectively. The degradation of docosane 11-decyl by, Bacillus methylotrophicus ALK-16, was 80%. Degradation of hexatriacontane by Enterobacter species ALK-23 was 31%. Further understanding of the metabolic processes of these organisms on crude oil hydrocarbons degradation will increase possibilities to develop strategies for removing crude oil pollutants from oil-impacted environments. Article Highlights • Bacterial isolates Alcaligenes species ALK-14 degraded docosane 11-decyl, hexatriacontane and eicosane by Alcaligenes species ALK-14 was 100%, 60% and 48.8 % respectively and the obtained degradation pattern was 11-decyl > hexatriacontane > eicosane. • Under optimized conditions, the parameters have showed synergistic effect therefore bacterial growth was enhanced up to 11-28% which is directly proportional to the hydrocarbon degradation. • Eighty percent degradation of docosane 11-decyl was achieved by Bacillus methylotrophicus ALK-16.