Evaluation of Moringa Oleifera Lam Leaves (Moringaceae) Diets Against Induced Anemia in Wistar Rats (original) (raw)
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Journal of Medical and Biological Science Research, 2020
The leaves of Moringa oleifera have exceptional nutritional qualities and are used in traditional medicine to treat several diseases including anemia. This study is to propose a formulation for the optimal use of M. oleifera leaf powder in the treatment of anemia in rats. Phenylhydrazine (40 mg/kg) was administered intraperitoneally for 2 days to induce anemia in rats (Rattus norvegicus). The animals were divided into six groups of 6 rats each and treated orally from day D2 to D28. Rats groups 1, 2, 3 and 4 were treated orally, respectively with 400, 800 and 1600 mg/kg of M. oleifera aqueous extract of leaves (AEMo) and Ranferon ®. Groups 5 and 6 were fed ad libitum with diets containing 50% (P50) and 100% (P100) of M. oleifera leaves as a substitute for soybean meal, respectively. Red blood cell (RBC) parameters such as RBCs, hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were analyzed on days D0, D2, D5, D14 and D28. Results showed a prevalence of 0% in anemic rats treated with P50, AEMo 1600 mg/kg and Ranferon ® versus 16.67% in those treated with P100, AEMo 400 and 800 mg/kg at D28. In addition, P50, AEMo 1600 mg/kg and Ranferon ® significantly (p ˂ 0.001) increased RBCs, Hb, Ht with total recovery especially RBCs and Ht in anemic rats treated with P50 and Ranferon ®. In conclusion, the incorporation in small quantities of M. oleifera leaf powder as a substitute for soybean meal has a better efficacy on anemia than the total leaf extract of the same plant.
Anti-Anemia Effect of Standardized Extract of Moringa Oleifera Lamk. Leaves on Aniline Induced Rats
Pharmacognosy Journal, 2016
Background: The leaves of Moringa (Moringa oleifera Lamk.) have been empirically used as anti-anemia for pregnant woman. Objectives: In the present study, the effect of ethanolic extract of moringa leaves was evaluated on the hematology parameters (hemoglobin, red blood cell count, hematocrit), on aniline induced white female rats. Materials and Methods: Thirty female rats strain Sprague-Dawley were divided into six groups: normal, anemia and ferrous fumarate group, and three groups of Moringa leaves extract. All groups, except normal group, induced by aniline at dose 0.005 ml/ g bw intraperitonially. On fifth day, normal and anemia groups were given 0.5% CMC, ferrous fumarate group was given ferrous fumarate orally and three other groups were given Moringa leaves extract at doses 198 mg, 396 mg, and 792 mg/200 g bw until the eleventh day. Results: The results showed that as Moringa leaves extracts increased the level of hemoglobin, red blood cell count, hematocrit, and total iron content in blood. Moreover, Moringa leaves extract at dose 792 mg/200 g bw can improve red blood cell's morphology and increased the level of hemoglobin and red blood cell count significantly (p<0.05). Conclusion: The ethanolic extract of Moringa leaves showed anti-anemia on aniline-induced rats.
This study evaluated the effectiveness of dry Moringa oleifera leaf powder in the management of anaemia in adult albino rats. The proximate, mineral, vitamin and phytochemical composition of dry M. oleifera leaf powder were analysed. Twelve adult albino rats grouped into three were used. Cyclophosphamide was used to induce anaemia into them. The percentage proximate values were protein (26.28%), ash (7.69%), carbohydrate (49.35%), crude fibre (7.48%) and moisture (7.05%). The rats whose feed were supplemented with M. oleifera leaf powder showed superior attributes to the unsupplemented group. The study showed dry M. oleifera leaf powder is promising in the management of anaemia.
This study was aimed at investigating the effects of Moringa oleifera leaf-meal (MOLM) on some haematological parameters of male albino rats. Sixteen (16) albino rats were randomly selected into four (4) groups; a control group (group A) and three experimental groups (groups B, group C and group D) containing four (4) animals per group. Group A was given normal diet, groups B, C and D were provided with MOLM diets containing 37.5 gm, 56 gm and 75 gm respectively. They were fed with water ad libitum daily for 21 days. At the end of the experimental period, haematological parameters (white blood cell count, packed cell volume, red blood cell count, haemoglobin count and differential white blood cells) were determined. The result showed significant differences (p<0.05) in hemoglobin count, red blood cells and packed cell volume in group B while there was a significant difference (p<0.05) in lymphocyte count of animals in group C and D, although all the groups were within normal range while no significant difference (p >0.05) was shown in the white blood cell count when compared to the control group. In conclusion, this study revealed that MOLM possesses nutritional benefits and this was indicated by its effects on RBC, PCV, haemoglobin and lymphocyte of the animals.
Corresponding author. e-mail: Hisham1212ribat@yahoo.com. This study is aimed at finding out the effect of Moringa oleifera on blood parameters and body weights of albino rats (n= 24) and rabbits (n= 10). The rats were divided into four groups; a control group and three experimental groups, while the rabbits were divided into two groups; a control group and an experimental one. The three experimental groups of rats were provided consecutively with100, 200 and 300 mg M. oleifera leave extract/kg of body weight daily for 21 days, while the experimental group of rabbits with 2.5 g fresh leaves of M. oleifera/Kg of body weight which was added to their feed daily for 21 days, and the control groups were fed on their diets without M. oleifera. The results showed significant differences (P < 0.05) in mean cell hemoglobin concentration (MCHC) and platelets (PLT) in the third group of rats (AL 3) and red blood cells (RBCs) count, hemoglobin (Hb) and MCHC in the fourth group (AL 4) while no...
The study was designed to investigate the effect of ethanol leaf extract of Moringa oleifera Lam. in phenylhydrazine-induced anemic albino Wistar rats. Twenty five (25) rats of both sexes were randomly assigned to 5 groups. Group 1 (normal control), Group 2 (negative control) was challenged with Phenylhydrazine (40 mg/kg, i.p.) without treatment. Group 3 received M. oleifera extract at 300 mg/kg. Groups 4 and 5 were challenged with phenylhydrazine (40 mg/kg) and treated with 300 and 600 mg/kg of M. oleifera respectively. All treatments with the extract were per os. All animals were allowed free access to food and water pre and post treatment for 21 days. At the end of the treatment period, blood samples were collected from the rats via the retro-orbital plexus of the eye. The hematological parameters assayed for were red blood cell count, hemoglobin count, white blood cell count, packed cell volume, platelet count, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration and lymphocytes count. Changes in body weight of the rats were also determined. Results showed that there was significant (P<0.05) increase in some blood parameters (red blood cell count, hemoglobin count, white blood cell count). Body weights were also found to increase with increasing doses of the extract in the PHZ-challenged animals. In conclusion, the oral administration of ethanol extract of M. oleifera has the tendency to increase some blood parameters and may be important in the treatment and management of anemia especially hemolytic and hemorrhagic anemia.
Role Of Moringa Oleifera Leaf Extract In Increasing Hemoglobin Levels In Pregnant Rats With Anemia
Journal of Health Science, 2021
Anemia is one of the epidemic health problems in society and the most common nutritional problem. The Moringa oleifera is rich in excellent dietary content and can complement the body's needs. This study aims to determine Moringa oilefera leaf extract's effectiveness in increasing hemoglobin levels in pregnant rats (Ratus norvegicus) with anemia. This paper used a true experimental research design with a pre-post test randomized control group design. This study utilized female rats (Ratus norvegicus), pregnant 8-12 weeks, weighing 200 grams. Experimental procedures were four treatments with three repetitions. Types of treatment were negative control and positive control (administration of Moringa oilefera leaf extract) at a dose of 0.18 g / head, 0.36 g / head, and 0.72 g/head. Analysis using one way ANOVA with an error rate of 0.05. The results showed p<0.005, which means a significant increase in hemoglobin levels in the group given the treatment of moringa leaf extract...
INFLUENCE OF PROBIOTICATED MORINGA OLEIFERA LEAF EXTRACT FOR TREATMENT OF ANAEMIA USING ANIMAL MODEL
An attempt was made to evaluate anti-anaemic properties of probiotic Moringa oleifera leaf extract using animal model. Twenty four male rats were randomly assigned to four groups and all the animals were given Phenylhydrazine orally to induce anaemia. Enterococcus durans was selected as probiotic supplement. Group 1 was provided with 300mg/kg of M.oleifera leaf extract. Group 2 was supplemented with 0.3 ml of E.durans along with 300mg/kg of M. oleifera leaf extract. Group 3 and 4 were fed with E.durans and iron supplement (1ml/rat) respectively. Blood samples were collected on a weekly basis and various haematological and biochemical parameters were analysed. The haemoglobin level was found to be higher with 14.9±0.25 g/dl for rats fed with probioticated M.oleifera leaf extract. E.durans has proliferated in gut which was verified through faecal and gut microbiota analysis. Hence, probiotic M.oleifera leaf extract was found an effectual nutraceutical supplement to treat anaemia.
2021
Moringa oleifera, grown mainly in the tropical and subtropical regions of the world is commonly known as ‘drumstick leaves’ or ‘horseradish leaves’. Leaves, as well as all other part of the Moringa tree are edible and they form traditional diets in any countries. Because of its high nutritive value, every part of the tree is suitable for either nutritive or commercial purposes. The leaves are found to be rich in minerals, vitamins and other essential phytochemicals that can be used to treat malnutrition, control blood glucose and prevent anemia. This review explores the use of moringa leaves across disciplines for its medicinal values and deals with its supplemental effect on blood glucose, malnutrition and anemia.
Utilization Potentials of Moringa oleifera Leaves Syrup in Prevention of Anemia
https://ijshr.com/IJSHR\_Vol.4\_Issue.4\_Oct2019/IJSHR\_Abstract.001.html, 2019
Background: Anemia is still remains as the main nutritional problem in Indonesia. Based on Indonesia basic health research 2018, the prevalence of anemia in Indonesia was 23,7%. The prevalence of anemia in children under 5 years was 38.5%, and in pregnant women was 48,9%. Indonesia has a large number of natural plants that contain rich of nutrients such as Moringa oleifera. The leaves are 25 times more iron than spinach. Aims: This study aims to analyze the content of Fe level and the organoleptics of Moringa oleifera leaves syrup as an alternative food product in prevention of anemia. Methods: This was an experimental study using a completely randomized design (CRD) consisting of one factor and 3 repetitions. The samples were: Moringa oleifera leaves mixed with pineapple as treatment samples and Moringa oleifera leaves without pineapple as control. The 5 points hedonic scales were conducted for the organoleptic test: dislike extremely, dislike, neither like nor dislike, like, like extremely assessed by 30 panelists. The Fe level assessed by spectrophotometers. Results: The highest average of color preference, aroma, taste test and Fe level was Sample 1. The treatment samples had good aroma and a acceptable taste because they were mixed with pineapple which contains Bromelin enzyme which can reduce the bitter taste of Moringa oleifera leaves. Conclusions: Syrup of Moringa oleifera leaves mixed with pineapple could be an alternative food product in prevention of anemia. Keywords: syrup, Moringa oleifera, prevention, anemia