Cobalt chloride induces hepatotoxicity in adult rats and their suckling pups (original) (raw)
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International Journal of Zoology and Animal Biology, 2019
The excess application of COC may have an adverse effect on both animals and human population to better understand the maternal toxicity estimated in pregnant female albino rats, COC administered orally at different doses (1/10 and 1/20 LD50 of COC equivalent to 147 mg/kg bow and 73.5 mg/kg Bw. respectively) daily from day 6 th to day 15 th of pregnancy with daily observation and weighing. The pregnant dams were sacrificed on day 20 th of gestation and blood and tissue samples collected for hematological, biochemical, histopathological, ultrastructural changes examination besides copper residues level determination in the liver tissues. The results showed that there was a significant dose-dependent decrease in the maternal body weight with respect to the control group besides a significant decrease in the metabolic parameters as glucose, total protein, and cholesterol however, there was a significant increase in the liver enzymes, urea and creatinine furthermore there was a severe decrease in the antioxidant biomarkers as SOD, CT, GSH and GST with a significant increase in the level of MDA revealing oxidative stress additionally the results found that the was a sever histopathological and ultrastructural changes in the liver tissue and hepatocytes. Furthermore, the level of copper in the liver tissue increased significantly in treated dams in comparison to control. Such results revealed the potential maternal toxicity of COC fungicide in a dose-dependent manner in female albino rats after the accumulation of copper in the liver tissue that considered as the main target organ.
Biological Trace Element Research, 2013
Chronic overexposure to cobalt (Co) may result in neurotoxic effects, but the mechanism of Co-induced neurotoxicity is not yet well established. Our study was conducted to determine whether Co is associated to the induction of central nervous system damage in pregnant rats and their progeny. Twelve pregnant female rats were randomly divided into 2 groups: group I served as controls and group II received Co (350 mg/L, orally). Treatments started from the 14th day of pregnancy until day 14 after delivery. Co concentration in plasma was higher in the treated groups than in the controls. Exposure to Co also increased the levels of MDA, PCO, H 2 O 2 , and AOPP, while Na + K +-ATPase and Mg 2+-ATPase, AChE, and BuChE activities decreased in the cerebrum and cerebellum of suckling pups. A smear without ladder formation on agarose gel was also shown in the cerebrum and cerebellum, indicating random DNA degradation. A reduction in GPx, SOD, CAT, GSH, NPSH, and vitamin C values was observed. The changes were confirmed by histological results. In conclusion, these data showed that the exposure of pregnant and lactating rats to Co resulted in the development of oxidative stress and the impairment of defense systems in the cerebrum and cerebellum of their suckling pups.
Postnatal toxicity of copper oxychloride in lactating female albino rats
2020
Objective: To evaluate the postnatal toxicity of copper oxychloride (COC) in lactating female albino rats. Design: Randomized controlled experimental study. Animals: Eighteen pregnant female albino rats weight 150±10 g and 6-7 week old. Procedures: Eighteen pregnant female albino rats were divided into 3 groups treated orally with copper oxychloride 0, 73.5, 147 mg/kg (equivalent 1/20 and 1/10 of LD50) daily from first day of parturition for 21 days. Female rats and its offspring were euthanized at 21 days of treatment. The postnatal toxic effect in the neonates and dams were estimated through biochemical biomarkers as metabolic and oxidative stress biomarkers, histopathological and hematological evaluation. Results: There was a significant increase in liver enzymes activities as ALT and GGT and oxidative stress biomarker as MDA (P < 0.05) in both suckling pups and lactating dams beside decrease the level of metabolic biomarkers as glucose, total protein and cholesterol (P < 0...
Effect of Carbon tetrachloride on Liver enzymes and Lipid Peroxidation in Rat
International Journal of Current Microbiology and Applied Sciences
In this present study, 12(twelve) albino rats divided into two groups. Each group consisting of three males and three females. T1 for control group received only liquid paraffin @ 3ml/kg.b.wt. on alternate day for four injections. T2 group treated with carbon tetrachloride @ 1ml/kg.b.wt. following dilution with liquid paraffin at the ratio of 1: 2 i.e. 3ml/kg.b.wt. subcutaneously on alternate day for four injections. T2 group rats showed decreased reduced glutathione(5%), increased lipid peroxidation(60%) and decreased SGOT(62.4%). The pathological changes in the liver tissue showed fally changes, coagulative necrosis, cirrhosis and hyperplasia of bile duct.
2015
Bu calisma, Aroclor 1254’e maruz kalan gebe ratlarin beyin, bobrek ve karaciger dokularinda hem oksidant hem de antioksidant parametreleri arastirmak amaciyla yapilmistir. Calismada ratlar iki gruba ayrilmistir ve her bir grupta 10 rat bulunmaktadir. Birinci grup, gebe kontrol grubu ve ikinci grup, gebe Aroclor 1254 (2 mg/kg/gun, Aroclor 1254’un bu dozu 20 gun boyunca subkutan olarak uygulandi). Her grupta yer alan gebe ratlar eter anestezi yontemi ile uyutulmus ve karaciger, beyin ve bobrek dokulari cikarilmistir. Bu dokularda lipit peroksidasyon parametresi malondialdehid (MDA), glutatyon (GSH) seviyeleri ile glutatyon peroksidaz (GSH-Px), katalaz (CAT), superoksit dismutaz (SOD), glutatyon reduktaz (GR), glutatyon S transferaz (GST) aktiviteleri olculmustur. Calisma sonucunda karaciger, bobrek ve beyin dokularinda analizi yapilan parametreler acisindan iki grup arasinda istatistiksel acidan bir anlamli bir fark tespit edilememistir. Yapilan calisma ile gebelik suresince uygulanan...
Pediatrics international : official journal of the Japan Pediatric Society, 2002
The aim of this study was to assess the response to dioxin-induced oxidative stress in neonates via lactation in the model we have described previously. Maternal rats were treated with a single dose of 50 or 100 micro mol/kg 1,2,3,4-tetrachlorodibenzo-p-dioxin (TCDD) on the first day postpartum (day 1). Messenger RNA levels of the key anti-oxidant enzymes (AOE), phospholipid hydroperoxide-glutathione peroxidase (PH-GPx), cellular-glutathione peroxidase (cell-GPx), copper-zinc superoxide dismutase (CuZn SOD), manganese superoxide dismutase (Mn SOD) and catalase (CAT) in the neonatal and maternal livers were determined by a competitive reverse transcription- polymerase chain reaction method. Lactational transfer of 1,2,3,4-TCDD induced an inhibition of PH-GPx and cell-GPx mRNA in the neonatal liver on day 2 to 68 (P < 0.01) and 62% (P < 0.05) of the control at 100 micro mol/kg, respectively. Both GPx mRNA returned to control levels on day 6 and thereafter increased to levels hig...
Environmental Toxicology, 2013
Potassium dichromate (K 2 Cr 2 O 7) is an environmental contaminant widely recognized as a carcinogen, mutagen, and teratogen toward humans and animals. This study investigated the effects of K 2 Cr 2 O 7 on the hepatic function of pregnant and lactating rats and their suckling pups. Experiments were carried out on female Wistar rats given 700 ppm of K 2 Cr 2 O 7 in their drinking water from the 14th day of pregnancy until day 14 after delivery. Hepatotoxicity was objectified by the significant increase in liver malondialdehyde content and a significant accumulation of chromium in this soft tissue. Moreover, exposure to K 2 Cr 2 O 7 induced a decrease of glutathione, nonprotein thiols, and vitamin C in the liver of mothers and their suckling pups. Alteration of the antioxidant system in the treated group was confirmed by the significant decline of antioxidant enzyme activities such as catalase, glutathione peroxidase, while liver superoxide dismutase activity increased in mothers and decreased in their offspring. It was found that K 2 Cr 2 O 7 induced liver damages as evidenced by the elevation of plasma aminotransferases, lactate dehydrogenase activities, and bilirubin levels. Impairment of the hepatic function corresponded histologically. Our investigation revealed hemorrhage, leukocytes infiltration cells, and necrosis, which were more pronounced in the hepatocytes of mothers than in those of their suckling pups. # 2011 Wiley Periodicals, Inc. Environ Toxicol 00: 000-000, 2011.
Effects of ferro sulphate and carbon tetrachloride in liver of rat
Journal of the Medical Sciences, 2015
Ferro sulphate (FeSO4) overload and carbon tetrachloride (CCl4) are chemical hepatocarcinogen. Ferro sulphate disrupts the redox balance of the cell and generates chronic oxidative stress by which modulates signaling networks related to malignant transformation. Meanwhile CCl4 induces hepatic damage in lipid peroxidation and decreases activities of antioxidant enzymes and generation of free radicals. The aim of this study is to investigate the effect of a pause of chemical hepatocarcinogen induced in Rattus norvegicus rats. Twomonths old adultmaleRattus norvegicus rats weighing around 110–191 g were used. The ratswere divided into three groups. In group I (n=3), no-treatment control; in group II (n=3), rats were fed 3.5%FeSO4 in the diet together with 0.1 ml/ kgBWCCl4 administered by gavage per os 5 days a week for 3 weeks. However, in group III (n=3), rats were administered by chemical hepatocarcinogen like group I then continued with no-treatment for 2 weeks (a pause of 2 weeks wa...