Preparation of Silk Fibroin Nanofibres by Needleless Electrospinning using Formic Acid-Calcium Chloride as the Solvent (original) (raw)
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Effect of Calcium Chloride on Electrospinning of Silk Fibroin Nanofibres
2012
Silk fibroin is one of the candidate materials fo r biomedical application because it has good biocompatibility and minimal inflammatory reaction. Electrospinning is a simple method capable of prod ucing nanofibres for biomedical applications. This study was focused on the production of a nonwoven sheet f rom silk fibroin using a needleless electrospinning method a nd concentrated on an alternative way of spinning s olution preparation by using a mixture of formic acid and c alcium chloride as a solvent. The effects of salt c oncentrations in formic acid and the voltage of electric field on fi bre morphology were studied. It was observed that n anofibre has a good uniform fibre distribution on the nonwoven she et by increasing the applied voltage. Calcium chlor ide increases the solubility of silk fibre in formic acid with 2 wt% calcium chloride being preferred. The silk nano fibres had diameters ranging from 200 to 2300 nm.
Fabrication of Silk Fibroin Nanofibres by Needleless Electrospinning
Electrospinning - Material, Techniques, and Biomedical Applications, 2016
Silk fibroin nanofibres were fabricated using a needleless electrospinning technique. The procedure focused on a new method for the preparation of a spinning solution from silk fibroin. The role of the concentration of silk fibroin solution, applied voltage and spinning distance were investigated as a function of the morphology of the obtained fibres and the spinning performance of the electrospinning process. The biocompatibility of the obtained fibre sheets was evaluated using an in vitro testing method with MG-63 osteoblasts. The solvent system consisted of formic acid and calcium chloride that can dissolve silk fibroin at room temperature, and a rate of 0.25 g of calcium chloride per 1 g of silk fibroin was required to obtain a completely dissolved silk fibroin solution. The diameters of the silk electrospun fibres obtained from the formic acid-calcium chloride solvent system ranged from 100 to 2400 nm, depending on the spinning parameters. Furthermore, increasing the concentration of the silk fibroin solution and the applied voltage improved spinning ability and spinning performance in needleless electrospinning. In addition, in vitro tests with living cells showed that the obtained electrospun fibre sheets were highly biocompatible with MG-63 osteoblasts.
Fabrication of Silk Nanofibres with Needle and Roller Electrospinning Methods
Journal of Nanomaterials, 2014
ABSTRACT In this study, silk nanofibres were prepared by electrospinning from silk fibroin in a mixture of formic acid and calcium chloride. A needle and a rotating cylinder were used as fibre generators in the spinning process. The influences of the spinning electrode and spinning parameters (silk concentration and applied voltage) on the spinning process, morphology of the obtained fibres, and the production rate of the spinning process were examined. The concentration of the spinning solution influenced the diameter of the silk electrospun fibres, with an increase in the concentration increasing the diameters of the fibres in both spinning systems. The diameters of the electrospun fibres produced by roller electrospinning were greater than those produced by needle electrospinning. Moreover, increasing the concentration of the silk solution and the applied voltage in the spinning process improved the production rate in roller electrospinning but had less influence on the production rate in needle electrospinning.
Needleless Electrospinning of Silk Fibroin / Polycaprolactone Blend Nanofibres
2014
In this study, composite electrospun fibres consisting of silk fibroin and polycaprolactone was fabricated by needleless electrospinning method. The electrospinning of silk fibroin/ polycaprolactone blends with different composition ratios was performed with formic acid as a spinning solvent. The effects of blend ratio of silk fibroin/polycaprolactone in spinning solution is investigated as a function of the properties of spinning solution, the morphology of electrospun fibres and the spinning performance of electrospinning process. Result showed that an increase in the weight ratio of polycaprolactone in spinning solution produces a significant effect on the fibre diameter of the electrospun fibre and the spinning performance of process. With an increase in the amount of polycaprolactone in spinning solution decreasing the fibre diameter and the spinning performance of electrospining process. The silk fibroin/ polycaprolactone blend fibres had diameters ranging from 200 to 1100 nm.
Electrospun silk fibroin using aqueous and formic acid solutions
MRS Advances
Silk fibroin is a polymer of interest thanks to its ability to be transformed into different structures, such as fibers. The electrospun technique can produce micro and nanofibers, presenting advantages like high superficial area and porosity. However, this polymer needs to be dissolved into a liquid solution using solvents. This study evaluates the effect of formic acid and water as solvents on the silk fibroin electrospun fibers morphology, chemical structure, and thermal properties. In this case, silk fibroin was obtained from silk fibrous wastes. The results suggest that the morphology obtained from both solutions has a similar fiber diameter. Electrospun silk fibers using formic acid solution present a relatively high porosity and recrystallization enthalpy. In contrast, the percentage of crystallinity and degradation temperature were higher in samples with aqueous solution. This indicates that the aqueous process allows higher structural ordering, improving the thermal stability for the fibers.
Silk fibroin from silk fibrous waste: characterization and electrospinning
IOP Conference Series: Materials Science and Engineering
In this work, the effect of fabrication parameters on silk fibroin (SF) nonwovens obtained by electrospinning were evaluated. Additionally, the relationship between secondary structures and thermal stability of the protein materials, with morphological characteristics of nonwovens obtained were analyzed. Silk fibroin in formic acid solution at 10% w/w was electrospun at 8 cm varying the ratio voltage/distance and flow rate. The nonwovens morphology was observed by Scanning Electron Microscopy (SEM) and fibers diameter were determined with ImageJ software. The changes in the secondary structure of silk fibroin before and after electrospinning were studied by Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) to evaluate the effect of electrospinning process in the molecular structure of SF. Optimum morphology of SF nonwovens were obtained at R = 1 kV/cm and low flow rate, these process parameters were related to higher contents of crystalline structures in the materials. Results showed that SF nonwovens obtained under controlled process parameters hold great potential to be utilized in several applications such as tissue engineering.
2016
Silk fibroin protein derived from Bombyx Mori silk cocoon is a natural polymer that widely used for application in tissue engineering. Silk Fibroin has remarkable properties that demonstrates biocompatibility, biodegradable and great mechanical properties. In this present study, regenerated silk fibroin can be obtained by multistep preparation which are degumming and solubilisation process. The small pieces of regenerated silk fibers were cut and then blended with Polyvinylalcohol (PVA) solution to enhance the mechanical property of scaffold. Electrospinning capable to fabricate scaffold with the high surface area. Result showed that by increased of concentration of solution silk/PVA, voltage and flowrate, more fibers were produced in the film. A thin white film was produced at film of silk/PVA at 14% (w/v) without addition of chloroform. Scanning Electron Microscope (SEM) investigated the morphology of silk and revealed that applied voltage of 15kV during electrospinning has narrow...
Fabrication and characterization of nanofibrous scaffold developed by electrospinning
Materials Research, 2011
Electrospinning has been recognized as an efficient technique for the forming of polymer nanofibers. Silk fibroin (SF) nanofibers were electrospun from SF solution using trifluoroacetic acid solution as a solvent. In the present work, we have systematically evaluated the effects of instrument parameters, including applied voltage, tip-target distance, solution flow rate, solution parameters; such as polymer concentration and solution viscosity on the morphology of electrospun SF fibers. The applied voltage and flow rate was monitored at fixed tip target distance during the electrospinning process and it was correlated with the characteristics of the fibers obtained. The number of deposited fibers also increases with the applied voltage. Also, viscosity, flow rate and applied voltage strongly affect the shape and morphology of the fibers. A particular interest, we demonstrated that by monitoring the applied voltage and flow rate it is possible to control the fibers morphology and bead concentration. Rheological study showed a strong dependence of spinnability and fiber morphology on solution viscosity. Solution concentrations has been found to most strongly affect fiber size, with fiber diameter increasing with increasing solution concentration and the morphology of the deposition on the collector changed from spherical beads to interconnected fibrous networks. FTIR analysis clearly shows that there are no spectral differences between fibers and which suggests that there was no chemical modification developed during the process. Under optimized conditions, homogenous (not interconnected) SF fibers with a mean diameter of 234 nm were prepared.
Review for application of electrospinning and electrospun nanofibers technology in textile industry
Journal of Nanostructure in Chemistry, 2016
Electrospinning (electrostatic fiber spinning) is a modern and efficient method which uses electric field to produce fine fibers which their diameter can reduce to nanometers. These fibers have wide applications in industry such as filtration, composite materials, medical, membrane, etc. In this paper a review of electrospinning process, its products and applications are explained.