Plasmid Curing and Antibiotic Susceptibility Test of Bacteria Isolated From RiverOsin, Ila Local Government Area, Ila- Orangun, Osun State, Nigeria (original) (raw)

Water is an essential natural resource and a basic need of lives. Its qualities and sustenance is threatened by pollution, resulting into serious public health concern. Polluted or contaminated water could harbor pathogenic microorganisms. Therefore, this study aimed at subjecting isolated bacteria from River Osin, Ila Local Government, Ila-Orangun, Osun State, Nigeria, to antibiotic susceptibility test and plasmid curing following standard microbiological procedures. The mean value of total viable bacterial count and total coliform count obtained ranged between {2.41x 10 3-1.81x10 4 }cfu/ml and {120-150}cfu/100ml for February and July, 2019 respectively and were both found to exceed WHO standard of 1.0x10 2 cfu/ml and (0) zero cfu/100ml respectively. A total of (9) nine bacteria were isolated and identified to include; Staphylococcus aureus, Escherichia coli, Klebsiellasp, Salmonella sp, Bacillus sp, Streptococcus sp, Proteus sp, Alcaligenssp, and Pseudomonas aeruginosa. All isolates tested for susceptibility were resistant to Cotrimaxazoleand Amoxicillin, and showed varied susceptibility to Levufuroxime, Tetracycline, Levofloxacin and Amikacim. Many were susceptible to Ciprofloxacin, Gentamicin and Ofloxacin. The plasmid curing done shows that the resistance genes were both plasmid and chromosomallyborne. It was concluded from this work that all bacteria isolated from this River resist Cotrimazole and Amoxicillin, and their resistant genes were both plasmid and chromosomally borne.