A Variety of Modern Methods for Detection and Typing of Human Papillomavirus (original) (raw)
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Introduction. High carcinogenic-risk human papillomaviruses (hrHPVs) are recognized as etiological agents of cervical cancer. Constant expression of the viral oncoproteins, E6 and E7, is required for maintenance of the malignant phenotype of tumor cells. The exact mechanism of regulation of viral oncogenes expression in tumor cells is not fully elucidated. The purpose: identification of viral noncoding RNAs (ncRNAs) in HPV16-positve cervical cancer. Materials and methods. The reverse transcription polymerase chain reactions were used to detect viral ncRNAs in HPV16-positve primary cervical squamous cell carcinomas and SiHa and CasKi cell lines. The knockdown technique with oligonucleotides complementary to ncRNAs was used to elucidate their functions. Results. We have identified ncRNAs transcribed in the upstream regulatory region of HPV16 in the cervical carcinoma cell lines and in 32 out 32 cervical squamous cell carcinomas with episomal or integrated forms of HPV16 DNA. Knockdown...
Detection of high-risk HPV genotypes using Real-time PCR
Glasnik javnog zdravlja
Discovery of the causal relationship between the human papilloma virus and cervical cancer formation increased the significance of the real-time PCR in HPV diagnostics. Based on evidence showing that they caused cervical cancer, 14 HPV types have been classified as carcinogenic (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). This study analysed cervical smears taken from female patients, aged 19 to 25 years, using the Viasure diagnostic test for the detection of high-risk HPV genotypes and individual identification of HPV genotypes 16 and 18. A total of 110 cervical smears were analysed and 44 positive samples were detected (40%). DNA analysis of the positive samples found the following distribution of the HPV types: 27% HPV (31, 39, 56); 22% HPV (52, 59, 68); 18% HPV16; 13% HPV (33, 45, 51); 12% HPV (35, 58, 66); 8% HPV18. This study and the high positivity rate it found indicate that there is a lack of awareness among the youth on the measures of prevention, as wel...
DOAJ (DOAJ: Directory of Open Access Journals), 2012
распространенность вируса папиллоМы человека высокого канЦерогенного риска в респуБлике саха (якутия), санкт-петерБурге и респуБлике карелия ФБУН НИИ эпидемиологии и микробиологии имени Пастера (Санкт-Петербург) Управление Роспотребнадзора по Республике Саха (Якутия) (Якутск) Всероссийский центр экстренной и радиационной медицины им. А.И. Никифорова МЧС России (Санкт-Петербург) Кожно-венерологический диспансер № 8 (Санкт-Петербург) Республиканский кожно-венерологический диспансер Республики Карелия (Петрозаводск)
Èpidemiologiâ i vakcinoprofilaktika, 2020
Обзор Review Г. Н. Минкина* ФГБОУ ВО Московский государственный медико-стоматологический университет им. А. И. Евдокимова Минздрава России, Москва Достижения и перспективы вакцинопрофилактики папилломовирусной инфекции и ассоциированных заболеваний Резюме Актуальность. Прошедшее десятилетие ознаменовано стремительными достижениями в области профилактики инфекций, вызываемых вирусом папилломы человека (ВПЧ) и ассоциированных заболеваний. Цель. В обзоре обобщен многолетний мировой опыт и рассмотрена доказательная база влияния национальных программ вакцинации против ВПЧ на распространенность ВПЧ-инфекции, заболеваемость генитальными кондиломами и предраковыми цервикальными поражениями в клинической практике. Представлены результаты модельных исследований эффективности различных стратегий вакцинации для создания коллективного иммунитета, гендерно-нейтральной вакцинации и потенциала для элиминации онкогенных типов ВПЧ. Вывод.
Proceedings of the Shevchenko Scientific Society Medical sciences
Cervical cancer (CC) is a serious demographic and medical problem in Ukraine, which is the main cause of the death of women in reproductive age in our country. WHO experts recognized the possibility of complete elimination of CC on the globe under conditions of implementation of primary measures (vaccination against papillomaviruses) and secondary (cytological screening) prevention. However, there still are many opponents to vaccination and ill-informed people, which limits the current possibilities of preventative measures in Ukraine. In 2017, for the fi rst time in Lviv region, 4,500 doses of Cervarix vaccine against human papilloma virus (HPV) were purchased for the vaccination of girls aged from 12 to 14. This opened new opportunities for studying the tolerance of vaccination, interest and awareness among young people on these issues. Aim: To analyze the activity of vaccination against HPV in Lviv area. To assess the level of student knowledge ofrisk factors related to the development of cervical cancer, as well as the feasibility of its prophylaxis, namely vaccination against HPV. Methods and material: The use of the bivalent vaccine Cervarix in the framework of the region programwas analyzed, namely the coverage of girls aged 12-14 by the vaccination based on the monthly reports frommedical institutions. Thesurveyresults of 200 respondents aged 17-32, most of whom are medical students, are analyzed. They are related to their awareness of the risk factors for CC, primary and secondary prevention measures.
Srpski arhiv za celokupno lekarstvo, 2014
The aim of this paper was to present the role of human papillomavirus (HPV) in cervical carcinogenesis from several aspects. By explaining the HPV virus lifecycle and structure, its effect on cervical cell cycle and subversion of immune response can be better understood. Early E region of the viral genome encodes proteins that are directly involved in carcinogenesis. The E6 protein binds to p53 protein (product of tumor-suppressor gene) blocking and degrading it, which in turn prevents cell cycle arrest and apoptosis induction. E6 is also capable of telomerase activation, which leads to cell immortalization; it also reacts with host proto-oncogene c-jun, responsible for transcription, shortens G1 phase and speeds up the transition from G1 to S phase of the cells infected by HPV. E7 forms bonds with retinoblastoma protein (product of tumor-suppressor gene) and inactivates it. It can inactivate cyclin inhibitors p21, p27, and abrogate the mitotic spindle checkpoint with the loss of pr...