Isolation of Halophilic Bacteria from Maharlu salt Lake -Iran and their evaluation for the production of bioactive compounds (original) (raw)
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Characterization of Halophilic Isolates Producing Bioactive Metabolites Against Pathogens
Lahore Garrison University Journal of Life Sciences, 2020
Halophiles are salt loving microorganisms that are able to survive in high salt conditions. Halophiles are highly diverse group belongs to all three domains of life, Archaea, bacteria and Eucarya. Halophiles adapted themselves through various strategies to maintain their osmotic balance in order to survive in high salt concentrations. Likewise halophiles are also able to produce bioactive molecules such as carotenoids, polyhydroxy-alkanoates, ectoine, bioplastics, and enzymes that have commercial importance. In current study halophiles were isolated at 1M NaCl concentration and characterized, and check its antibacterial activity against pathogenic E.coli, Pseudomonas aeruginosa and Klebsiella pneumonae. Two halophilic strains showed activity against pathogenic E.coli and Klebsiella pneumonae only. Antimicrobial activity was further analyzed through disc diffusion method and by using extracted filtrate. The activity only observed against E.coli after 48 hours old cultures that were s...
Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16 S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8 pH in the growth medium. Further the partially purified biosurfactants were characterized by TLC, FTIR and GC-MS analysis. GC-MS results revealed that, the partial purified biosurfactants contain 1, 2-Ethanediamine N, N, N', N'-tetra, 8-Methyl-6-nonenamide, (Z)-9-octadecenamide and a fatty acid derivative. Pharmacological screening of antibacterial, antifungal, antiviral and anticancer assays revealed that, the biosurfactant extracted from Halomonas sp BS4 effectively controlled the human pathogenic bacteria and fungi an aquaculturally important virus, WSSV. The biosurfactant also suppressed the proliferation of mammary epithelial carcinoma cell by 46.77% at 2.5 μg concentration. Based on these findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin.
Evaluation and Isolation of Halophilic Bacteria from the Meyghan Lake in Arak, Iran
Medical Laboratory Journal, 2018
Background and Objectives: Halophilic bacteria can grow and survive in environments with a wide range a wide range of salinities. In this study, we aimed to isolate halophilic bacteria from the Meyghan Lake in Arak (Iran) and evaluate their enzymatic activity. Methods: Samples were taken from four different areas of the lake. Halophilic bacteria were isolated by culture in moderate halophilic medium, sea water nutrient agar and nutrient agar containing different salt concentrations. Purification was done via consecutive culture methods, and production of extracellular hydrolytic enzymes including amylase, protease, lecithinase, DNase and lipase was evaluated. Results: Among 74 bacteria isolated from the lake water samples, 24 produced amylase, 27 produced lipase (Tween 40 and 80), 68 produced protease, three produced DNase and 61 produced lecithinase. Conclusion: In this study, we isolated halophilic bacteria with enzymatic activity and potential industrial applications.
Nature and bioprospecting of haloalkaliphilics: a review
The haloalkaliphilics are an important subset of extremophiles that grow in salt [upto 33% (wt/vol) NaCl] and alkaline pH (> 9). They are found in hypersaline environments especially in the brines in arid, coastal and deep sea locations, and in alkaline environments, such as soda soils, lakes and deserts. Some authors have described haloalkaliphilic bacteria as moderate halophilic bacteria, but the molecular and classical studies revealed that they belong to moderately to extremely halophilic bacteria and archaea. Organic solutes, such as glycine, betaine and other amino acid derivatives, sugars such as, sucrose and trehalose, and sugar alcohols present in the haloalkaliphilics help for their osmoadaptation, and also serve as stabilizers. Haloalkalphilics secrete exoenzymes like proteases, amylases, xylanases, cellulases and peroxidases which have potential industrial applications. They also produce bacteriorhodopsin, compatible solutes, pigments, biopolymers, secondary metabolites like biosurfactants, polyhydroxyalkanoate (PHA) and exopolysaccharides and antimicrobial/anticancer compounds. They have unique metabolic pathways which can be used to treat industrial pollutants, heavy metals and waste water.
Antibacterial activity of halophilic bacterial bionts from marine invertebrates of Mandapam-India
Indian Journal of Pharmaceutical Sciences, 2012
Velho-Pereira and Furtado: Antibacterial Activity of Halophilic Bacterial Bionts from Marine Invertebrates Marine ecosystem and its organisms, particularly the invertebrates are recent targets of bioprospecting and mining for a large group of structurally unique natural products encompassing a wide variety of chemical classes such as terpenes, polyketides, acetogenins, peptides and alkaloids of varying structures, having pronounced pharmacological activities. In view of the limited reports on the antibacterials produced by bacteria, isolated from marine sponges, corals and bivalves of Indian origin, the present study is aimed at investigating the antagonistic activities of 100 heterotrophic, halophilic bacterial bionts isolated from 9 sponges, 5 corals and one bivalve. Culture broths of 46 of these bionts were active against human pathogenic bacteria namely Staphylococcus citreus, Proteus vulgaris, Serratio marcesans, Salmonella typhi, Aerobacter aerogenes and Escherichia coli. Further, the ethyl acetate extracts of cell free supernatant confirmed the presence of extracellular bioactive factor, by agar cup diffusion method. Interestingly, highest number of bionts having activity was isolated from corals followed by sponges and bivalve. The study clearly demonstrates that bacterial bionts of marine invertebrates are a rich source of bioactive secondary metabolites against human bacterial pathogens.
Isolation and Characterization of Halophilic Bacteria from Sundarban Soil
Halophiles, the most predominant organisms found in the mangrove forest, include varied genera of halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majority of halophilic microorganisms studied so far produce compounds with great potential in industrial process and they have physiological properties which facilitate its use with commercial aims. In this study of Sunderban soil, focus has been made on the isolation of halophilic organisms and their characterisation. Soils were collected from four different places of Sunderban and were used for physiochemical and microbiological analysis. Qualitative screenings of the isolates were done and three among them was selected having moderately good growth which was further optimised in different growth media. Characterisation of the isolates were done and based on 16S rRNA gene sequencing phylogenetic tree were constructed. The isolates showed multiple heavy metal tolerance and antibiotic resistance. The organisms were further tested for EPS as well as various extracellular enzyme productions thereby exploring their usage for various biotechnological purposes.
Bacillus subtilis has ability to grow in extreme environments like Alkaliphilic, Halophilic. These environmental conditions induce microorganisms to produce varied kinds of antimicrobials which has applications in chemotherapy. Therefore In present study antibiotic producing ability of the halophilic and alkaliphilic Bacillus subtilis were compared by screening the activity of the antimicrobial compound was determined against different microorganisms like, Staphylococcus aureus, E. coli, P. aeruginosa, Candida tropicalis, Candida parapsilosis. The halophilic and alkaliphilic Bacillus subtilis isolates were isolated from Alkaline Meteorite Crater Lake Lonar situated in district Buldhana, India and were subjected to primary screening for antimicrobial compound production. The organisms were enriched in Alkaline and Halophilic NG medium which supports the production of phospholipid antibiotic by respective organism and partial purification was done using thin layer chromatography. The partially purified extract of antimicrobial phospholipid compound from both the organisms were tested against different microorganisms and seen that halophilic Bacillus subtilis showed less antimicrobial activity than alkalophilic Bacillus subtilis isolated from Lonar Lake which are having moderate antibacterial activity against different microorganisms.
Isolation, Characterization of Halotolerant bacteria and its biotechnological potentials
2013
1 Abstract— Marine microbes represent a potential source for commercially important bioactive compounds and their bioremediation capabilities are also remarkable. Hence for the present study the bacterial strains were isolated from salt enriched soils which were collected from the natu- ral saline habitats from Bhitarkanika coastal region of Orissa, India. The phenotypic characters of the isolates conclusively proved that isolates S1-4 belongs to Bacillus spp. and S5 belongs to Micrococcus luteus and S6 could not be identified, it would be a new isolate, which changed their shape to spherical forms when grown in NaCl which is a halotolerant character. The bacteria isolates (S 1-4) produced gummy (except S1) colonies of different shape, size margin and elevation. Bacterias were motile except S5, aerobic, gram +ve except S6, spherical and elliptical spores forming rods and cocci of (2.26 - 4.25) x (0.70-0.85) µm size range and catalase (+)ve. However, the organisms differed in some ph...
Int.J.Curr.Microbiol.App.Sci , 2015
Dead Sea is a hypersaline terminal desert lake with 34.8% salinity and is one of the extreme habitats for the sustenance of life on earth. Present ionic composition of the lake is 2M Mg 2+ , 0.5M Ca 2+ , 1.5M Na + , 0.2M K + , 6.5M Cl-and 0.1 M Br-, which is inhibitory to most of life forms. Dead Sea harbours numerous halophilic microorganisms such as algae, bacteria, archaea and fungi; which can withstand the extreme conditions of the lake. During the years of heavy rainfall such as 1980 and 1992, upper water layer of the Dead Sea becomes diluted and leads to development of algal blooms which is followed by bloom of red halophilic Archaea. Metagenomic studies revealed the significant difference between microbial community during the algal bloom and resident community during the interbloom period. Halophilic archaea and bacteria isolated from the lake include the members of the genera Haloferax, Haloarcula, Halobaculum, Halorubrum, Halomonas, Chromohalobacter and Salibacillus. The halophilic microorganisms are utilized for various biotechnological applications and present study focuses on the isolation and screening of halophilic microorganisms from the Dead Sea for biotechnologically important properties such as extracellular protease production, Polyhydroxy alkanoates (PHA) production, halocin production and bio emulsifier production. Highest extracellular protease activity of 12.45 U/ml and highest PHA concentration of 27.5 % of cell dry weight was obtained. Isolates showing halocin production were also tested against the human pathogens such as S. aureus and P. aeruginosa; while this is one of the few reports, describing the bioemulsifier production from the halophiles.