Determination of antioxidant activities of algae from lentic ecosystems under anthropogenic stress: A comparative study (original) (raw)
Related papers
2016
The main objective of this study was to investigate the in vitro antioxidant activities of three selected green (Codium tomentosum, Enteromorpha linza), red (Gelidium sesquipedale) and brown (Cystoseira spicata, Padina pavonica) seaweeds from western of Tripoli coast, Libya. After methanol extraction, polyphenol, flavonoids, and condensed tannins contents were assessed. Methanol extracts of the seaweeds were investigated for their total antioxidant activity, reducing power, DPPH radical scavenging activity, NO radical scavenging activity, deoxyribose scavenging activity, and nitric oxide radical inhibition assay. Brown seaweed especially C. spicata exhibited good antioxidant activity when compared to red and green seaweeds. The maximum antioxidant activity was shown by the methanol extract of C. spicata for DPPH and nitric oxide radicals scavenging assay (66%, and 53% respectively). Also C. spicata extract showed a noticeable protection levels against lipid peroxidation. However, ex...
Antioxidant activities of marine algae: A review
2012
Oxidative stress is the result of an imbalance between pro-oxidant and antioxidant homeostasis that leads to the generation of toxic reactive oxygen species (ROS). The necessity of compounds with antioxidant activity is increasing as it is realized that the formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been linked in the pathogenesis of several human diseases such as atherosclerosis, diabetes mellitus, chronic inflammation, neurodegenerative disorders and certain types of cancer. The antioxidant activity of these compounds are mainly attributed to scavenging activity against superoxide and hydroxyl radicals, chelating ability, quenching singlet and triplet oxygen, and reducing power. It is important to develop, identify and utilize new source of safe and effective antioxidants of natural origin. Recently, much research attention has been focused on the free-radicalscavenging activity of metabolites from marine macro algae. Several studies have investigated the antioxidant activity of natural products in marine and freshwater algae. The marine environment is known as a rich source of chemical structures with numerous beneficial health effects. Among marine organisms, marine algae have been identified as an under-exploited plant resource, although they have long been recognized as valuable sources of structurally diverse bioactive compounds. Here summarized what are the compounds, methods and recent research on antioxidant activities of marine algae.
Mineral Composition, Antioxidant and Antimicrobial Activities of Freshwater Algae (Spirogyra Genus)
Scientific Conference Proceedings, 2013
Dry biomass of algae is a good source of nutrients and biologically active substances, which in the recent years attracted the interest of the specialists in their search for natural, ecologically and healthy sound foods for the animals. The aim of the present study was to determine the mineral composition, the antioxidant and antimicrobial activities of the dry biomass of freshwater algae (spirogyra genus). The freshwater algae were collected in February and March 2010 at the pond of Jomo Kenyatta University of Agriculture and Technology (JKUAT). The Mineral content by acid digestion (HNO 3 :HClO 4) in mg / 100g was as high as 387.22 ± 38.72 for Ca and as low as 2.53 ± 0.48 for Cu. Phytochemical screening of methanolic and water extract showed the presence of alkaloids steroids, flavonoids, tannins, terpenoids, but only the water extract showed the presence of total reducing sugars. The content of phenolic compounds and tannins was respectively 10.63 ± 0.05g gallic acid/100g of extract and 0.399 ± 0.02g tannic acid/100g of dried sample and the level of flavonoids was 6.86 ± 0.14g Quercetin/100g of extract. The algal antioxidant activity expressed in a dose able to decrease the initial DPPH concentration by 50% was 0.078 mg/ml. The antimicrobial activity was found only for methanolic extract against Escherichia coli and Candida albicans but no antimicrobial activity for aqueous extract. The freshwater algae used in this study were identified as Spirogyra nitida associated Spirogyra weberi both filamentous green algae. The mineral composition showed that they contain minerals which give the possibility of using them as food for fish and also by human as food supplement. The levels of Ca, Mg and Fe were very high as the Spirulina and Chlorella are used as food supplement. The strains showed a relative antioxidant and antimicrobial activities.
International Journal of Pharmacy and Pharmaceutical Sciences, 2019
Objective: This work aimed to screen the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida). Methods: Evaluation of the antioxidant activity of different collected species, lyophilized and extracted with a solvent mixture chloroform/methanol (2/1; v/v) was conducted according to two techniques, first by thin layer chromatography (tlc) then by spectrophotometry, using a free radical 2,2-diphenyl-1-picrylhydrazyl (dpph). The sampling on a distance of 110 km allowed to harvest 86 algal species (16 brown algae, 47 red algae, 14 green algae and 9 algae being identified). Results: The analysis by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This activity varies depending on the concentration of the extract and in function of incubation time in the presence of dpph. The monitoring of the kinetics of degradation of dpph by spectrophotometer in the presence of extracts which were active by tlc allowed to confirm the results and select the most active algal species based on the percentage of remaining dpph in the medium after 120 min of reaction: Fucus spiralis (17.02 %), Cyctoseira ericoides (12.16 %) (Phaeophyceae), and Gracilaria multipartita (36%), Halopitys incurvus (5%) (Rhodophyceae). Conclusion:
Antioxidant Potentials of Marine Red and Green Algae Extracts In-vitro
Reactive oxygen species (ROS) is normally found in balance with antioxidant molecules within all aerobic cells. The unbalance condition between ROS and antioxidant molecules will cause oxidative stress that may lead to any damages on nucleic acid, protein, and fatty acid. In this study, we investigated the antioxidant potentials of Indonesian marine algae extracts, including red algae (Botryocladia sp. and Gracilaria sp.) and green algae (Caulerpa sertulaioides, C. racemosa, Codium sp., Enteromorpha sp., and Halimeda opuntia) in vitro. Seven marine algae were dried and extracted with ethanol, followed by evaporating and freeze-drying treatments to obtain algae extracts. The antioxidant activity was referred to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Our results demonstrated that both red algae (Botryoclardia sp. and Gracilaria sp.) and green algae (C. sertulaioides and Codium sp.) at 100 µg/mL significantly possessed 35-40% antioxidant activity, indicating that these marine algae extracts may exert potential natural antioxidant properties.
Phytochemical Screening, Antioxidant Activity and Total Phenolic Content of Spirogyra spp
Advanced Materials Research, 2013
A freshwater macroalga, Spirogyra spp., were analyzed for its phytochemical composition, antioxidant activity and total phenolic content (Folin−Ciocalteu method). Phytochemical analysis of Spirogyra neglecta revealed presence of phenolics, tannins, glycosides and saponins. The crude extraction of Spirogyra spp. was carried out using two solvents via, methanol (methanolic extract) and water (aqueous extract). The total phenolic contents of crude extracts were shown at 346.58±1.61 and 589.77±1.65 mg gallic acid equivalents/g extract in aqueous and methanolic extracts, respectively. The antioxidant capacity of aqueous and methanolic extract was estimated by two different methods; ABTS assay, and DPPH assay. The antioxidant activity of two extracts is affected by the extracting solvent and different assay. In the DPPH scavenging assay and ABTS assay, both extracts showed high antioxidant activity. In addition, the high correlation between results of all antioxidant activities and total ...
Effect of Solvent Extraction System on the Antioxidant Activities of Algae
International Journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: The present study aimed to determine the antioxidant activities of five algae including Oedogonium globossum, Pithophora oedogonia, Rhizoclonium hieroglyphicum, Scytonema ocellatum and Spirogyra communis and the effect of different solvent (benzene, chloroform, acetone and methanol) used for the extraction of active components from algae. Methods: The five algae were extracted with four different solvents e. g benzene, chloroform, acetone and methanol. The Folin-Ciocalteu procedure was used to estimate the amount of total phenolic content in different extracts of algae. Total flavonoid content was estimated by aluminium chloride colorimetric method. The DPPH radical scavenging assay was used to find out the antioxidant activities of algae under investigation. Results: The total phenolic content varied from 9.80±0.29 to 44.44±2.26 mg/g, 7.26±0.08 to 21.30±0.17 mg/g, 9.82±0.12 to 50.14±0.99 mg/g and 11.51±0.17 to 54.02±0.58 mg/g dry material in the benzene, chloroform, acetone and methanol extracts of the algae respectively. The flavonoid content was found to be the highest in the acetone extract of Scytonema ocellatum (593.81±2.69 mg/g) and flavonol content was found to be the highest in the chloroform extract of Scytonema ocellatum (448.22 ± 4.36 mg/g) whereas least amount of flavonoid present in the benzene extract of Oedogonium globossum 8.91±0.37 and least amount of flavonol was present in the chloroform extract of Spirogyra communis (13.54±1.07 mg/g). The highest DPPH radical scavenging was observed in the methanol extract of Scytonema ocellatum with IC50 = 0.24±0.003 mg dry material. The reducing power of the extracts of the algae was also evaluated as mg AAE (ascorbic acid equivalent)/g dry material. Chloroform extract of Rhizoclonium hieroglyphicum showed the highest reducing power 185.56±2.00 mg AAE/g dry materials. Conclusion: Thus it could be suggested that these algae can be utilized as natural antioxidant.
ANTIOXIDANT ACTIVITY OF FIVE SEAWEED EXTRACTS
New Knowledge Journal of Science, 2018
In this study, aqueous extracts of five seaweeds collected from Çanakkale, Turkey were studied for free radical scavenging activity and phenolic compounds. The extracts of two brown algae (Cystoseira barbata, Scytosiphon lomentaria), two green algae (Ulva rigida, Enteromorpha intestinalis) and one red algae (Gigartina acicularis) were prepared with boiling. The extract of S. lomentaria demonstrated greater antioxidant potential with a low IC50 (2,67 mg/g Ext.). It was also determined that the free radical scavenging activity of other seaweed extracts were close to this value. The total phenolics, flavonoids and carotenoids of the aqueous extracts ranged from 0,10 (G. acicularis) to 0,66 (C. barbata) mg/g Ext., 1,15 (S. lomentaria) to 1,30 (E. intestinalis) mg/g Ext. and 414,2 (U. rigida) to 2194,5 µg/g Ext. (C. barbata) mg/g, respectively. It was determined that seaweeds collected from Çanakkale exhibited high free radical scavenging capacity. We also found that, the aqueous extracts of brown algae C. barbata had high amounts of phenolic compound. According to our results, aqueous seaweed extracts could be use as organic fertilizers to increase antioxidant levels of agriculture products.
Zenodo (CERN European Organization for Nuclear Research), 2022
Free radicals interfere with the equilibrium of cells and tissues, which can lead to cancer. Fresh water algae such as Microspora tumidula and Lyngbya kützingii is a great source of secondary antioxidant metabolites. These metabolites most likely work well in the therapy of cancer. Algae exhibit huge variety of pigments not only chlorophyll, Carotenoids, phycobilins, and xanthophylls are the most prevalent of these. In the beginning, the medicinal effect of microalgae biomass was studied when it was used as pills, powder, and water additives. More and more studies in recent years have focused on finding and using useful medicinal components in algae. Aim of this study to evaluate the antioxidant role of algae in pharmaceutical industries. In the current investigation, the algal extracts were prepared by using three solvents methanol, chloroform and n-hexane to know about antioxidant potential of algae of specific area. To evaluate the antioxidant activity different test were performed such as DPPH, TAA, TPC, FRAP and MC. In Microspora tumidula 15.16% DPPH highest value was shown by methanolic extract. In FRAP Lyngbya kützingii showed maximum value in methanolic extract 64 µM Trolox mg-1. While the highest value of TPC by Lyngbya kützingii was shown in chloroform extracts 37.5µg GAE /mg. The results of total antioxidant activity (TAA) were showed that Lyngbya kützingii and Microspora tumidula both exhibited the highest value 154mg /g and 152mg/g respectively in methanolic extract. The result of metal chelating test showed highest value in chloroform extract 10.44% by Lyngbya kützingii. So both these algal species showed antioxidant potential.
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: This work aimed to screen the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida). Methods: Evaluation of the antioxidant activity of different collected species, lyophilized and extracted with a solvent mixture chloroform/methanol (2/1; v/v) was conducted according to two techniques, first by thin layer chromatography (tlc) then by spectrophotometry, using a free radical 2,2-diphenyl-1-picrylhydrazyl (dpph). The sampling on a distance of 110 km allowed to harvest 86 algal species (16 brown algae, 47 red algae, 14 green algae and 9 algae being identified). Results: The analysis by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This activity varies depending on the concentration of the extract and in function of incubation time in the presence of dpph. The monitoring of the kinetics of degradation of dpph by spectrophotometer in the presence of extracts which were a...