Haematological and biochemical alterations in calves following infection with pasteurella multocida Type B: 2, bacterial lipopolysacch aride and outer membrane protein immunogen (OMP) (original) (raw)
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The current study aims to investigate the Clinico-pathological responses of calves associated with the infections of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Alterations in the behavior of animals and pathological lesions observed following innate or experimental infections usually divulge extensive and detrimental changes in the clinical signs, organs and tissues of the animals afflicted with the disease. These alterations are imperative for Veterinary evaluation of herd health. Eight clinically healthy, non-pregnant and non-lactating Brangus cross heifers weighing 150±50 kg were used in the study. The heifers (n = 8) were divided into 4 groups of 2 calves per group. The control calves in group 1 were inoculated intramuscularly with 10 mL of sterile Phosphate Buffered Saline (PBS). Calves in group 2 were inoculated intramuscularly with 10 mL of 10 12 colony forming unit (cfu) of wild-type P. multocida and calves in gro...
International Journal of Animal and Veterinary Advances
The current study aims to investigate the Clinico-pathological responses of calves associated with the infections of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Alterations in the behavior of animals and pathological lesions observed following innate or experimental infections usually divulge extensive and detrimental changes in the clinical signs, organs and tissues of the animals afflicted with the disease. These alterations are imperative for Veterinary evaluation of herd health. Eight clinically healthy, non-pregnant and non-lactating Brangus cross heifers weighing 150±50 kg were used in the study. The heifers (n = 8) were divided into 4 groups of 2 calves per group. The control calves in group 1 were inoculated intramuscularly with 10 mL of sterile Phosphate Buffered Saline (PBS). Calves in group 2 were inoculated intramuscularly with 10 mL of 10 12 colony forming unit (cfu) of wild-type P. multocida and calves in group 3 were inoculated intravenously with 10 mL of LPS broth extract. Calves in group 4 were inoculated intramuscularly with 10 mL of OMP broth extract. All animals were observed for 48 h for clinical signs, changes in behavior and mortality pattern, including the time of death. The results divulged significant differences in the Clinico-pathological alterations. Calves inoculated with whole cell P. multocida type B: 2 showed a significant (p<0.05) increased in rectal temperature. The affected calves showed significant severe dullness (p<0.000) and significant rumen hypomotility (p<0.000) was also exhibited. The calves showed signs of hypersalivation at 14 h. There is no significant difference (p = 0.240) in pulmonary oedema in the Calves of group 2 compared to control group 1. Calves of group 4 also showed no significant difference in pulmonary oedema (p = 0.612) compared to control group 1. Calves of group 3 showed significantly moderate pulmonary oedema (p<0.000). All the three treatment groups showed significant (p<0.05) differences in the presence of inflammatory cells in the lung. All the three treatment groups showed significant (p<0.05) in the presence of degeneration and necrosis of cells in the lung. Calves of group 2 showed significantly severe haemorrhage (p<0.000) in the lung including groups 3 and 4 (p<0.000) respectively. Calves in group 2 showed significantly (p<0.000) mild thrombus formation. There is no significant thrombus formation in the lung of calves in groups 3 (p = 0.352) and 4 (p = 0.184) respectively. In conclusion, the pathophysiological changes in cattle will assist in the improvement of the vaccines and the vaccination methods that are currently employed in controlling this important disease in Malaysia.
2013
The current study aims to investigate the Clinico-pathological responses of calves associated with the infections of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Alterations in the behavior of animals and pathological lesions observed following innate or experimental infections usually divulge extensive and detrimental changes in the clinical signs, organs and tissues of the animals afflicted with the disease. These alterations are imperative for Veterinary evaluation of herd health. Eight clinically healthy, non-pregnant and non-lactating Brangus cross heifers weighing 150±50 kg were used in the study. The heifers (n = 8) were divided into 4 groups of 2 calves per group. The control calves in group 1 were inoculated intramuscularly with 10 mL of sterile Phosphate Buffered Saline (PBS). Calves in group 2 were inoculated intramuscularly with 10 mL of 10 12 colony forming unit (cfu) of wild-type P. multocida and calves in group 3 were inoculated intravenously with 10 mL of LPS broth extract. Calves in group 4 were inoculated intramuscularly with 10 mL of OMP broth extract. All animals were observed for 48 h for clinical signs, changes in behavior and mortality pattern, including the time of death. The results divulged significant differences in the Clinico-pathological alterations. Calves inoculated with whole cell P. multocida type B: 2 showed a significant (p<0.05) increased in rectal temperature. The affected calves showed significant severe dullness (p<0.000) and significant rumen hypomotility (p<0.000) was also exhibited. The calves showed signs of hypersalivation at 14 h. There is no significant difference (p = 0.240) in pulmonary oedema in the Calves of group 2 compared to control group 1. Calves of group 4 also showed no significant difference in pulmonary oedema (p = 0.612) compared to control group 1. Calves of group 3 showed significantly moderate pulmonary oedema (p<0.000). All the three treatment groups showed significant (p<0.05) differences in the presence of inflammatory cells in the lung. All the three treatment groups showed significant (p<0.05) in the presence of degeneration and necrosis of cells in the lung. Calves of group 2 showed significantly severe haemorrhage (p<0.000) in the lung including groups 3 and 4 (p<0.000) respectively. Calves in group 2 showed significantly (p<0.000) mild thrombus formation. There is no significant thrombus formation in the lung of calves in groups 3 (p = 0.352) and 4 (p = 0.184) respectively. In conclusion, the pathophysiological changes in cattle will assist in the improvement of the vaccines and the vaccination methods that are currently employed in controlling this important disease in Malaysia.
Clinico-pathological changes in buffalo calves following oral exposure to Pasteurella multocida B:2
Basic and Applied Pathology, 2011
Background and aim: Pasteurella multocida B:2, which causes hemorrhagic septicemia of ruminants, is believed to enter the host via respiratory and oral routes. While the role of respiratory route of infection has been established, the present study describes the clinical and pathological alterations following oral exposure of buffalo calves to live wild-type Pasteurella multocida B:2. Methods: Nine 8-month-old buffalo calves were selected and divided into three groups. Calves of group 1 were exposed orally to 50 mL of the inoculums containing 109 colony-forming unit (CFU)/mL of live Pasteurella multocida B:2. Calves of group 2 were exposed intra-tracheal to 5 mL of the same inoculums while calves of group 3 were exposed orally to 50 mL of phosphate-buffered saline. Results: Only one calf had to be euthanized at 48 h post-intra-tracheal exposure due to persistent clinical signs of hemorrhagic septicemia. The mean respiratory rate and rectal temperature had significantly increased in calves of groups 1 and 2. The pathological alterations included submandibular and brisket edema, generalized lymphadenopathy, acute fibrinous pneumonia, acute colitis and hemorrhagic typhilitis and proctitis. Nevertheless, oral infection with 109 CFU/mL of live wild-type P. multocida B:2 failed to produce a typical clinical disease. However, P. multocida B:2 was present along the gastro-intestinal tract, including the rectum of the calf that succumbed to the infection at 48 h post-intra-tracheal exposure. Conclusions: The presence of P. multocida B:2 along the gastro-intestinal tract is of concern since transmission via the gastro-intestinal tract of diseased animals is strongly possible.
2008
This study was carried out on 250 slaughtered cattle at Ahvaz abattoir in Khouzestan province of Iran to investigate the occurrence of Pasteurella multocida carriers and relationship with their immunity status. Nasopharyngeal swabs and 10 ml blood samples were taken immediately after slaughter. The swabs were streaked on 5% sheep blood agar plates. Cultures were incubated at 37oC for 24h and the plates were examined for colonies resembling P. multocida. Suspicious colonies were further subcultivated and examined microscopically and biochemically. The isolates were serotyped serologically and their pathogenicity in mice was carried out. Sera samples were tested for the presence of antibody against P. multocida by indirect haemagglutination (IHA) test and sera with a titer of |“1:16 were considered as positive. P. multocida was isolated from the nasopharynx of 6 (2.4%) out of 247 healthy cattle examined. There was no relation between infection and sex or age. All of 6 isolates belonge...
Journal of Comparative Pathology, 2010
Pasteurella multocida A:3 is a common cause of suppurative bronchopneumonia in calves and results in significant production losses and mortality. Here we describe the lesions in three calves at each of four time points (1 day and 4, 7 and 10 days) after experimental intratracheal infection with approximately 1 Â 10 9 colony-forming units of P. multocida A:3 Moredun Research Institute (MRI isolate 671/90). Equivalent age-and time-matched sham-dosed negative control animals were also studied. Infected calves developed significantly elevated mean rectal temperatures (P < 0.001) and respiratory rates (P < 0.001) compared with negative control animals. Extensive consolidation of multiple lung lobes was present on each of the day/s post-infection (dpi). Histologically, large numbers of alveoli contained either or both polymorphonuclear neutrophils (PMNs) and oedema fluid (1 dpi). At 4 dpi a severe fibrinosuppurative bronchopneumonia had developed. At this time, PMNs and macrophages formed focal lesions containing central necrotic and mineralized debris, while the interlobular septa were severely distended by oedema. Early abscess formation was present in the lung parenchyma at 7 dpi and many of the interlobular septa were thrombosed. At 10 dpi abscesses within the lung parenchyma were mature and comprised of central necrosis with surrounding layers of PMN, macrophages and fibrous tissue. This study describes, for the first time, the commencement, nature and progression of lesions in bovine pneumonic pasteurellosis caused by P. multocida A:3 and provides the foundations for further investigation of the pathogenesis of this disease in cattle.
Isolation of pasteurella multocida from cows and buffaloes in Urmia's Slaughter House
Pasteurellosis is one of the important economic diseases in ruminants, especially in cows and buffaloes. It is caused by Pasteurella multocida and occasionally by Mannheimia haemolytica. The aim of this study was to isolate Pasteurella multocida from lungs with probable mentioned bacterial agents in cows and buffaloes in Urmia's slaughter house. 240 lung samples over a period of 12 months were cultured. The results have revealed 6 (2.5 %) Pasteurella multocida results suggest that the animal, its breed, sex, age and season can be effective in the occurrence of these positive cases. The positive samples were all from male beef cattle of hybrid breeds (4 samples) in winter and Holstein breeds (2 samples) in spring.
Specific Antibodies of Pasteurella multocida in Newborn Calves of Vaccinated Dams
2001
Twenty-¢ve newborn Holstein Friesian calves, from dams vaccinated against haemorrhagic septicaemia (HS), were tested repeatedly over the ¢rst 6 months of life to monitor the transferred antibody levels against HS. Enzyme-linked immunosorbent assays were used to measure the speci¢c HS antibodies with antigens from Pasteurella multocida strains B:2 and E:2. There was a signi¢cant curvilinear relationship between the monitored IgG response and the age of the calves. Peak serum IgG levels were obtained during the period from 8 to 16 weeks of age. Beyond this age, the concentration of IgG in the serum fell away.