Intracellular trafficking of MytiLec, a Gb3-specific R-type mussel lectin with cytotoxic activity (original) (raw)

Abstract

A 17 kDa MytiLec isolated from the Mediterranean mussel was found to have a novel triple tandem primary structure and affinity to an alpha-galactoside, Gb3 (Gala1-4Galb1-4Glc-Cer). The lectin specifically killed Gb3-expressing Burkitt lymphoma Raji cells dose- and glycan-dependently [1]. A crystallographic study showed that MytiLec had beta-trefoil conformation which is a characteristic of Ricin B-chain type (R-type) family lectins, however, the amino acid sequence was not similar. Analogous to Ricin, which killed cultured cells expressing Galb1-4GlcNAc as the ligand, we administered MytiLec to four cultured cell lines that expressed Gb3 glycans and each cell line showed different degrees of dose-sensitivity against MytiLec. FITC-labeled MytiLec was administrated to these cells and its incorporation was observed by confocal laser scanning microscopy. Despite expression of Gb3 on the cell surface, some cell lines incorporated the lectin into the cytosol whereas others did not. Our re...

Prof. Dr. S. M. Abe Kawsar hasn't uploaded this paper.

Let Prof. Dr. S. M. know you want this paper to be uploaded.

Ask for this paper to be uploaded.