Osmotic Fragility and Erythrocyte Size in Iguana iguana (Sauria – Iguanidae) in Captivity (original) (raw)
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Comparative Haematology International, 1998
The osmotic fragility and erythrocyte size were studied on 80 blood samples (40 of each) obtained from both genus Caiman species present in Argentina, maintained in captive condition in the Northeastern region of the country (Province of Corrientes). The animals were of different size, age and sex, and were fed an artificial diet for five days a week. Samples were obtained by means of venepuncture of the caudal vein or by cardiac puncture, depending on the size of the animal. The osmotic fragility test was carried out according to Dacie's method. The maximum globular resistance was proved to be 20%, the minimum 40%, and the median was 28%. The cunmlative and derivative osmotic fragility curves were constructed. No significant differences were observed in the osmotic fragility from either species or sex, nor for differing ages. In addition, the erythrocyte diameter was measured, recording the largest and smallest diameters. The largest diameter was 17.00 + 3.5 #m and the smallest 9.00 + 1.8 #m in both species. Finally, the values obtained for osmotic fragility and erythrocyte diameters were compared with other vertebrate species, especially mammals and reptiles.
Animal Biology, 2009
Amphibians have long been known to display wide variation in erythrocyte morphology across species, but within species there has been little attention given to individual variation in red blood cell morphology. We captured 49 red-backed salamanders ( Plethodon cinereus ) from central Pennsylvania, USA and used image analysis procedures to measure erythrocyte morphology (size and shape) on blood smears made from all individuals. We then statistically examined whether variation in snout-vent-length, sex, tail loss, or capture location infl uenced these cell variables. Only snout-vent-length aff ected erythrocyte size and shape, with increasing body sizes associated with increasing cell areas and increasingly rounder cells. Further, erythrocyte shape was also associated with a measure of body condition that was corrected for body size, such that individuals with high body condition scores had rounder cells. Given the oxygencarrying role of erythrocytes in all vertebrates, we suspect this discovery is related to size-related changes in oxygen demand, since total oxygen consumption increases with body size in an allometric manner. While our results warrant further investigation to understand the mechanism, the association we found between cell roundness and both body size and condition nevertheless indicates this parameter could be used to assess the health state of plethodontid salamanders in future research, provided non-destructive sampling is employed. Our results also underscore the value of hematological investigations in the study of animal biology.
Integrative Zoology, 2013
Blood cell morphology and count are not uniform across species. Recently, between-species comparisons revealed that the size of red blood cells is associated with body size in some lizard taxa, and this finding was interpreted in the context of the metabolic theory. In the present study, we examined the numbers and the size of blood cells in 2 species of monitor lizards, the mangrove-dwelling monitor (Varanus indicus) and the savannah monitor (V. exanthematicus), and we compared these traits in individuals of different body size. The results revealed that during the course of ontogeny, the size of red blood cells increases with body mass. Because the mass-specific metabolic rate decreases with body size and the cell volume-to-surface ratio decreases with the cell size, changes in the erythrocyte size might be the result of oxygen transport adjustment. P. Frýdlová et al. P. Frýdlová et al.
Erythrocyte osmotic fragility and erythrocyte sizes for captive African rock pythons (Python sebae)
Comparative Clinical Pathology, 2019
The aim of the experiment was to determine the erythrocyte sizes and osmotic fragility index of the African rock python in a transitional zone. Ten African rock pythons (ARPs) were used as experimental animals. Blood samples were obtained from captive ARP by venepuncture of the ventral coccygeal vein. Erythrocyte counts, packed cell volume, mean corpuscular volume and erythrocyte sizes were determined. Erythrocytes obtained from each snake were added into saline solution of different NaCl concentrations of 0.1, 0.3, 0.5, 0.7 and 0.9 g/100 mL, respectively. Erythrocyte osmotic fragility index was determined spectrophotometrically. Mean erythrocyte size was 76.9 ± 10.2 μm and nucleus size was 9.1 ± 2.4 μm. The mean erythrocyte count obtained was 3.3 ± 0.6 × 1012/L, the mean packed cell volume obtained was 24.0 ± 2.9%, and the mean corpuscular volume was 76.4 ± 4.9 fl. The percentage haemolysis increased significantly (P < 0.05) at 0.3 g/100 mL NaCl concentration with the value of 79.9 ± 5.6%. The result of this finding adds to already available haematological data of the ARP and also showed that their erythrocyte may be extremely fragile. More research should be done on the effect of antioxidants in improving erythrocyte fragility of ARPs in the region.
Russian Journal of Theriology, 2020
The size of erythrocytes varies widely across mammals. Previously, deviations from allometric relationships and existence of factors regulating erythrocyte size other than body mass have been shown. The contribution of factors such as habitat and taxonomy are still under discussion. In the present study we examined the morphology of erythrocytes in rodent species and determined their diameter, and for Ondatra zibethicus and Sciurus vulgaris this was done for the first time. We discovered that erythrocyte diameter of the investigated rodent species ranged from 5.5 to 8.4 μm, varying by a factor of more than 1.5. We analyzed our own data obtained for 10 species as well as data from the literature for 22 species. We found that the size of erythrocytes depended on the phylogenetic position, environmental conditions and body mass.
Comparative Clinical Pathology, 2006
In the present work, we examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barcelos, Amazonas, Brazil). We identified erythrocytes, erythroblasts, thrombocytes and four types of leukocytes (basophils, heterophils, lymphocytes and monocytes) in the blood of these stingray species. In all the freshwater stingray species studied, the shapes and dimensions of these cells were similar to those of marine elasmobranchs. Positive PAS staining occurred in heterophils and thrombocytes, and weak staining occurred in lymphocytes and monocytes, while metachromasia only occurred in basophils. Positive Sudan Black B staining was observed in thrombocytes and lymphocytes, and weak staining occurred in heterophils. Basophils and heterophils were the only cells with positive bromophenol blue staining, while no peroxidase staining was observed in any of the four leukocyte types. This is the first study to establish the dimensions and cytochemical staining profiles of blood cells in Amazonian stingray species. Because these elasmobranch species are exported as ornamental fish to countries worldwide, this study can contribute to establishing standards for blood constituents that may be helpful in assessing the health and welfare of these fish in artificial systems. The subfamily Potamotrygoninae is a unique elasmobranch group composed of freshwater stingray species distributed along most of the great fluvial systems of South America ending at the Atlantic Ocean or Caribbean Sea 1-3. There are four genera of freshwater stingrays: Plesiotrygon, Paratrygon, Potamotrygon 4 and Heliotrygon 5. However, great effort and research investment are needed to achieve a better understanding of the diversity and taxonomic status of this family 6. Freshwater stingrays are an important component of Amazonian biodiversity. They have great socioeconomic importance, especially because of their use in the international ornamental fish trade and because they represent an alternative source of income for riverine communities living along the tributaries of the middle Rio Negro basin 7. There is a relationship between freshwater stingrays and fishermen, especially because stingray stingers
Comparative Morphology, Development, and Function of Blood Cells in Nonmammalian Vertebrates
Journal of Exotic Pet Medicine, 2009
... Topics in medicine and surgery. Comparative Morphology, Development, and Function of Blood Cells in Nonmammalian Vertebrates. Juan A. Claver DVM, PhD a , Corresponding Author Contact Information , E-mail The Corresponding Author and Agustin IE Quaglia b. ... Much of our knowledge regarding vertebrate blood and blood cells is based on mammalian references. The critical study of nonmammalian vertebrate blood is relatively new, and comparatively few investigations have been published that focus on these animals’ ontogeny and structure-function relationships of blood cells. Nonmammalian vertebrates comprise birds, reptiles, amphibians, and fishes, all of which have a wide range of forms and adaptations. For the clinician, a lack of basic knowledge with these species makes the evaluation of a hemogram more challenging than in mammals. This is a concise review of our current knowledge of comparative morphology that describes routine staining procedures and the development and function of blood cells in nonmammalian vertebrates. Copyright 2009 Elsevier Inc. All rights reserved..
Instructions for …, 2010
In this study morphological characterization of leukocyte and erythrocyte were investigated in central Asian tortoise (Testudo horsfieldii) in Iran. 7 males and 7 females were used in this study. Erythrocyte and leukocyte morphology were described using Wright´s technique. The sizes of erythrocyte and their nuclei were measured using an ocular micrometer under objective lenses (100x). Results of this study were compared with previous work on the other reptile species. Because there were no significant differences between the erythrocyte size of female and male tortoise, the data from the females and males of individual species were pooled. The mean length of mature erythrocytes was 1.98 µm (± 0.16 SD, with a range 1.10 -2.50 µm).The mean width of mature erythrocytes was 1.10 µm (± 0.14 SD, with a range of 0.80 -1.90 µm).The mean length of nucleus was 0.71 µm (± 0.09 SD, with a range of 0.50 -1.00 µm). The mean width of the nucleus was 0.52 µm (± 0.07 SD, with a range of 0.40 -0.70µm). Five types of leukocytes were found in the tortoise blood namely basophiles, eosinophils, lymphocytes, heterophils and monocytes.