Molecular Diagnostic of Anaplasma marginale in Carrier Cattle (original) (raw)
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Molecular Diagnostic of< em> Anaplasma marginale in Carrier Cattle
Background: Anaplasmosis belongs to the complex of several tick-borne diseases and can cause diseases in the livestock with high economical losses. Cattle that recover from acute infection become carriers and the parasite can persist most probably for the lifetime in the blood. The aim of the present study was the determination of the persistently infected cattle in a region of Iran with the previous history of acute anaplasmosis. Methods: One hundred and fifty blood samples and corresponding blood smears of cattle without any signs of diseases were prepared from a region in Isfahan/ Iran with the previous history of acute anaplasmosis from March 2007 to July 2007 for cross sectional study of carriers of Anaplasma. The blood smears were first screened by Giemsa staining, the extracted DNA from blood cells were analyzed by Anaplasma marginale specific nested PCR, and PCR-RFLP using primers derived from 16S rRNA gene and restriction endonuclease Bst1107 I. Results: Anaplasma like stru...
Molecular detection of Anaplasma spp. in cattle of Talesh County, North of Iran
BULGARIAN JOURNAL OF VETERINARY MEDICINE
Anaplasmosis is generally caused by intraerythrocytic rickettsia of Anaplasma genus and transmitted biologically and mechanically. The current study was designed to determine the prevalence of Anaplasma spp. in cattle in Talesh; one of the rainy Iranian counties in Gilan province, Iran. From May to November 2015, one hundred and fifty blood samples of cattle were collected from different regions in Talesh. DNA was extracted from blood samples and subsequently, 16S rRNA and MSP4 genes were analysed by Nested-PCR method for differentiation of Anaplasma spp. The results showed that 40.66% of blood samples were positive for Anaplasma spp. and that 24.66%, 35.33%, 9.33% and 12% of positive samples were infected with A. phagocytophilum, A. bovis, A. marginale and A. centrale respectively. Statistical analysis by Chi-square test did not show any significant relationship between the presence of Anaplasma species and variables sex, age and tick infestation (p˃0.05). The 4 species of Anaplasm...
Asian Pacific Journal of Tropical Biomedicine, 2017
To determine the prevalence of Anaplasma marginale (A. marginale) and Anaplasma ovis from sheep and goat in different highland pasture in west of Iran. Methods: From July 2015 to October 2015, 370 blood samples of sheep and goat were collected from different regions in Hamedan province, Iran. The DNA extracted from blood and subsequently, 16S rRNA and MSP4 genes were analyzed by nested-PCR, semi nested-PCR and RFLP methods. Results: In the PCR assessment, overall 27.5% (102/370) of sheep and goat were positive for Anaplasma ovis and A. marginale infection, which was lower than reports from tropical and subtropical regions of Iran. Statistical analysis (the Chi-square test) did not show any significant relation between infection and variables such as location, tick infestation age and sex (P > 0.05). No significant correlation between the altitude and the Anaplasma species infection was found (Mann-Whitney test: P > 0.05). However, Anaplasma infection in goat significantly is more than the sheep (P = 0.008). Conclusions: The ecological changes affect the frequency and distribution of Anaplasma species. Furthermore, our results indicate that sheep as potential reservoirs of A. marginale.
Acta Veterinaria Eurasia
The present study was carried out to determine Anaplasma species and potential risk factors associated with molecular prevalence of Anaplasma spp. among dairy cattle in southwest of Iran. A total of 88 samples out of 200 generated an expected amplicon of 866 bp from Anaplasma marginale msp4 gene. Six samples that were identified as A. marginale gave also positive results for A. phagocytophilum 16S rRNA gene with specific nested polymerase chain reaction (nPCR). The multivariate analysis of risk factors revealed that the cattle of mountain regions were significantly (p=0.0001) at higher risk as compared to the plain regions. Cattle <1 year age and the latitude 32-33°C were significantly at lower risk (p<0.01). The cattle with low milk yield were significantly (p=0.002) at lower risk. Low hygienic farms were significantly (p=0.011) at higher risk as compared to good and normal hygienic farms. Distance from other farms (<1Km) was another important risk factor which showed significant association with the occurrence of Anaplasma infection (p=0.021). The results of this study can be used in strategic planning for prevention and control of bovine anaplasmosis in dairy cattle in the southwest of Iran. Cite this article as: Noaman, V., Moradi, M., 2019. Molecular Epidemiology and Risk Factors Assessment of Anaplasma spp. on Dairy Cattle in Southwest of Iran. Acta Vet Eurasia; 45: 30-36. ORCID IDs of the authors: V.N. 0000-0002-3002-2417; M.M. 0000-0002-7713-9567. Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus). Veterinary Parasitology 63, 319-329. [CrossRef ]
Anaplasma Infection in Ticks, Livestock and Human in Ghaemshahr, Mazandaran Province, Iran
Journal of arthropod-borne diseases, 2014
Anaplasmosis is an important issue for animal breeders in terms of economic losses as well as a health concern to human. Ticks are considered as the main vector of this disease. Lack of documented information about Anaplasma species in Iran was the scope of this study to determine the population of ticks and the presence of Anaplasma in ticks, domestic ruminants and also human beings in northern Iran. A total of 101 unengorged hard ticks, 78 domestic ruminants and 40 human blood samples collected from Ghaemshahr, Mazandaran Province, northern Iran were tested by nested PCR against 16s rRNA gene of Anaplasma species. Positive PCR was found in 50 ticks, 28 sheep, 2 cattle, one goat, and 10 human specimens. Sequence analysis of the PCR products confirmed presence of A. ovis in two Rhipicephalus sanguineus and two Ixodes ricinus ticks, one human and 4 sheep samples. Moreover one Boophilus annulatus tick and one sheep sample were infected with A. bovis. Furthermore one sample of sheep wa...
Anaplasmosis, a tick-borne disease with worldwide distribution, impacts ruminants, equines, carnivores, and humans. This study aimed to investigate Anaplasma phagocytophilum in horses from Ardabil province and Anaplasma ovis in small ruminants from East Azerbaijan province using the Nested Polymerase Chain Reaction (PCR) method. Blood samples were taken from the jugular vein of 100 healthy horses in the Ardabil province and 156 healthy sheep and goats (116 sheep and 40 goats) in the East Azerbaijan province during the spring and summer seasons of 2016 in northwest Iran. The collected blood samples were stored at-20°C until the molecular experiments were conducted. Nested PCR was employed to detect A. phagocytophilum in horses and A. ovis in small ruminants using extracted DNA and amplifying 16S rRNA and msp4 genes. The Chi-square test of independence was used to determine the relationship between Anaplasma spp., infection, and independent variables, including age, gender, animal species, and sampling location. None of the 100 samples collected from horses in the Ardabil province were positive for A. phagocytophilum. In the East Azerbaijan province, 11 out of the 156 (7.05%) blood samples collected from sheep and goats tested positive for A. ovis. In addition, A. ovis infection was not significantly related to the independent variables. Phylogenetic analysis showed that the sequence obtained in this study (MH790273) had 100% homology with the sequence obtained from sheep infected with Anaplasma in Ahvaz province (JQ621903.1). The findings of this study can contribute to the prevention and control of anaplasmosis in farm animals in northwestern Iran.
2016
This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma marginale in sheep and dairy cattle in West-Azerbaijan province, Iran. A total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). The extracted DNA from blood cells was screened using genus-specific (Anaplasma spp.) nested-polymerase chain reaction (PCR) based on 16S rRNA gene primer sets. Species-specific PCR was set up using major surface protein 4 (MSP4) gene primer set. None of cattle blood samples were positive for Anaplasma spp. by the first nested PCR. Five samples among the 100 sheep blood samples were both positive in the first nested PCR and A. ovis -specific PCR, based on MSP4 gene. In total, 5.00% of animals were A. ovis positive. This study identified a low prevalence of A. ovis in the blood of apparently healthy sheep in West Azerbaijan province.
Journal of Scientific Research and Reports, 2015
Aim: Anaplasmosis is an important economic livestock disease. Limited information on its epidemiology in Iran is still lacking thus the aim of the study was to determine the seroprevalence of the disease in sheep and cattle in Kurdistan province of Western Iran with an overview of one decades of its epidemiological status in Iran. Study Design: This was a mixed cross sectional and longitudinal study carried out for a period of July to September 2013. Using competitive enzyme-linked immunosorbent assay (c-ELISA), for anti-Anaplasma antibodies. Methodology: A total of 182 blood samples were collected from 105 cattle and 77 sheep for the detection of antibodies against Anaplasma species using cELISA method. For this purpose, cattle and sheep of different sex and age were examined. Results: Examination of 182 blood samples revealed that 8 (7.62%) and 5 (6.49%) of cattle and 27 sheep were infected with Anaplasma species, respectively. Also, the prevalence of Anaplasma infection in relation to age and sex was not statistically significant.
Discrimination Between Anaplasma marginale and Anaplasma ovis by PCR-RFLP
2013
Abstract: Several tick-borne diseases with different etiological agents such as rickettsia, protozoa, bacteria and viruses can infect domestic ruminants. The only common feature between these diseases is that they can all be transmitted by ticks. Of these, Anaplasma spp. can cause diseases in the livestock with high economical losses. The Giemsa staining of blood smear is the common traditional method for the identification and characterization of anaplasmosis in Iran. The most specific method for the differential diagnosis of anaplasmosis is the method of polymerase chain reaction. Three hundred blood samples and corresponding blood smears of 150 cattle and 150 sheep without any signs of diseases were prepared from a region in Isfahan / Iran with the previous history of anaplasmosis. The blood smears were first analyzed by Giemsa staining and DNA extraction was performed only on blood samples with presence of Anaplasma spp. in marginal point of erythrocytes in their blood smears. T...
Molecular detection of Anaplasma bovis in cattle from central part of Iran
2010
Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran. The presence of A. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-PCR) based on 16S rRNA gene. Of the 150 cattle, 4 (2.66 %) was positive for A. bovis by nested-PCR. These data is the first A. bovis DNA presence in cattle from central part of Iran.