Cardioprotective effects and antioxidant status of Andrographis paniculata in isoproterenol-induced myocardial infarction in rats (original) (raw)
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Acta poloniae pharmaceutica
Present study evaluated the cardioprotective effect of Andrographis paniculata (100, 200 or 400 mg/kg) against isoproterenol (85 mg/kg, b.w.)-induced cardiotoxicity referred as myocardial infarction in rats. Isoproterenol significantly (p < 0.05) decreased mean arterial pressure, heart rate, contractility and relaxation and increased left ventricular end diastolic pressure. Isoproterenol also significantly (p < 0.05) decreased antioxidants, superoxide dismutase, catalase, glutathione peroxidase, glutathione and increased leakage of cardiac injury markers; creatine phosphokinase-MB isoenzyme, lactate dehydrogenase concomitant to increased lipid peroxidation and histopathological perturbations. However, pretreatment with A. paniculata favorably restored hemodynamic parameters and left ventricular function and significantly (p < 0.05) prevented the depletion of endogenous antioxidants and myocyte marker enzymes as well as inhibited lipid peroxidation. Significant (p < 0.05)...
Antioxidant Activity of Andrographis paniculata in Ischemic Myocardium of Rats
Global Journal of …, 2009
Hydroalcoholic extract of Andrographis paniculata prevented isoproterenol induced increase in lipid peroxidation and increased the activities of antioxidant enzymes viz. super oxide dismutase, catalase, glutathione peroxidase and the levels of reduced glutathione in hearts. In addition, the extract also prevented the leakage of lactate dehydrogenase from heart and salvages the heart from isoproterenol induced myocardial ischemic injury. The results indicate the antioxidant, antilipid peroxidative and antiischemic activity of A. paniculata and justify its use in ischemic heart diseases.
Journal of Pharmacy & Pharmacognosy Research, 2024
Context: Cardiovascular diseases claim the lives of an estimated 17.9 million people worldwide (report by the World Health Organization), yet the drug pipeline compared to some other life-threatening diseases, including cancer and neurological disorders, is low. Aims: To investigate the potential of Andrographis paniculata as a supplementary therapy for cardiovascular diseases based on recent in vivo animal studies. Methods: This study adopted a systematic review approach to analyze preclinical evidence from in vivo animal studies. Three databases (PubMed, Scopus, and Embase) were searched using the keywords “Andrographis paniculata”, “cardiovascular disease”, “CVD”, “heart disease”, “cardioprotective”, “cardio*”, “inflammation”, “oxidative stress”, “obesity”, “lipopolysaccharide”, “hypertension”, “arrhythmia” and “aortic disease”. The search period was from April 20th, 2023, to April 26th, 2023, and included studies published from 2013 to 2023. Only in vivo animal studies were appraised. In contrast, clinical studies, in vitro studies, in silico studies, and review papers were excluded. SYRCLE’s risk of bias tool was used to assess the risk of bias. Results: Sixteen eligible in vivo animal studies showed that Andrographis paniculata extracts and isolated bioactive compounds have strong anti-inflammatory and antioxidant effects on cardiovascular diseases. These effects lead to lowering the risk of coronary artery disease and myocardial infarction, easing the effects of bad cardiac remodeling, stopping cardiac hypertrophy, and improving diabetic cardiomyopathy. Although SYRCLE's tool detected some bias, the studies were included since they met the inclusion criteria and had no conflicts of interest. Conclusions: Andrographis paniculata may have the potential to be used as a supplementary therapy for cardiovascular diseases, but more animal studies and clinical trials should be done to establish these findings.
Chronic Effects of Andrographis paniculata Chloroform Extract in Spontaneously Hypertensive Rats
2012
This study was undertaken to investigate the effects of chronic administration of chloroform extract of Andrographis paniculata, known to possess some cardioprotective action, on endothelial dysfunction using isolated spontaneously hypertensive (SH) rat thoracic aorta. Verapamil hydrochloride was used as a positive control to compare its effect on endothelial dysfunction to that of Andrographis paniculata chloroform extract (APCE). HPLC study of APCE was performed and compared with that of the standards, andrographolide (ANG), and 14deoxyandrographolide (DA). Adult male SH rats were treated with 25, 50 and 100 mg/kg APCE and 12.5 mg/kg Verapamil (p.o.) once a day for four weeks. There was a significant (P<0.05) reduction in tail-cuff systolic blood pressure (SBP) measurements in conscious animals treated with 50 and 100 mg/kg APCE as well as in those treated with Verapamil. Similarly, these groups showed endothelium-dependent relaxation to acetylcholine (ACh). Animals treated wit...
Asian Journal of Pharmaceutical and Clinical Research
Objective: Cardiovascular diseases are the major cause of morbidity and mortality in the modern era. Myocardial infarction is a condition where there is a significant decrease or block in the blood (oxygen) supply to the part of heart, leading to degeneration of a portion of the myocardium which triggers a cascade of cellular, inflammatory and biochemical events, leading eventually to the irreversible death (necrosis) of heart muscle cells. Various therapeutic interventions, including lifestyle modification, pharmacological treatment options, and surgical techniques are available. The present review focus on the plants that have been evaluated for cardioprotective activity against isoproterenol-induced myocardial infarction.Method: The current status of Cardioprotective plants was obtained from a literature search of electronic databases such as Google Scholar, Pubmed and Scopus up to 2017 for publications on medicinal plants used against isoproterenol-induced myocardial infarction...
International Journal of Biology, Pharmacy and Allied Sciences, 2020
The main aim of this investigation is to investigate the cardioprotective effect of ethanolic extract of Ipomoea biloba on isoproterenol induced myocardial infarction in rats. The rats were divided into five groups of six animals each. Group I served as a normal control, Group II rats were administered isoproterenol (20mg/kg) through sub cutaneous injection to induce the myocardial infarction. Group III and IV were pretreated with ethanolic extract of Ipomoea biloba leaf (100mg/kg, 200mg/kg respectively) before to the subcutaneous injection of isoproterenol (20mg/kg, b.w) for 2 consecutive days. Group V received ethanolic extract of Ipomoea biloba (200mg/kg b.w) alone for 28 days excluding the treatment procedures. After the completion of experimental period, the levels of cholesterol, triglycerides, phospholipids, and lipoproteins were estimated. The myocardial damage was assessed by quantifying the serum levels of cardiac marker enzymes (AST, ALT, LDH, CK, and TP) uric acid, and ceruloplasmin. The results of this experiment revealed that the Isoproterenol induced rats showed a significant increase in the levels of triglycerides, total cholesterol and phospholipids in both serum and heart homogenate. A rise in the levels of LDL, VLDL with significant decrease in the level of HDL was also observed in the serum of isoproterenol-intoxicated rats. Interestingly our findings were proved that the treatment with
Evaluation of Hepatoprotective Activity of Aqueous Extract of Andrographis paniculata in Wistar Rats
International journal of pharmacology and clinical sciences, 2017
Background: The objective of the current study was to evaluate the hepatoprotective activity of the aqueous extract of Andrographis paniculata in wistar rats. Materials and Methods: The animals were divided into six groups with six rats in each group. First group was taken as control, and received 0.9% normal saline 10 ml/kg body weight orally. Second group was taken as CCl 4 control group and treated with normal saline (10 ml/ kg, p.o.) and CCl 4 : olive oil (1:1, 2 ml/ kg, i.p. on 2, 5 and 8 th day) daily for 20 day. The third group rats was treated with Liv 52-100 mg/ kg orally and CCl 4 : olive oil (1:1, 2 ml/ kg, i.p. on 2, 5 and 8 th day) for 20 days .The fourth and fifth groups were taken as test groups and they received crude extract of Andrographis paniculata orally once daily for 20 days at the dosage of 250 and 500 mg/kg respectively. CCl 4 : olive oil was given i.p on 2, 5 and 8 th day. On the 21 st day, blood samples were collected by cardiac puncture method. The blood samples were used to estimate biochemical parameters like serum SGOT,SGPT, ALT, bilirubin and cholisterol and the liver was preserved for histopathological examination. Results: It was found that the Andrographis paniculata exhibited moderate protective effect by lowering the serum levels of SGOT,SGPT,ALT, bilirubin and cholisterol (P<0.001). Histology of liver section of animal treated with the extracts showed the hepatic cell regeneration. Conclusion: Andrographis paniculata has dose dependant hepatoprotective activity.
BioMed Research International, 2015
We investigated the protective role ofWithania somniferaleaf extract (WSLEt) on isoproterenol- (ISO-) induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg body weight (b.w.)) administered to rats for two consecutive days caused a significant increase in cardiac troponin I (cTnI) levels and serum lipid profiles, as well as the activities of some marker enzymes. In addition to these diagnostic markers, there were increased levels of lipid peroxidation (LPO) and decreased activities of enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), and glutathione-S-transferase (GST)) in the myocardium. However, oral pretreatment (100 mg/kg b.w.) with WSLEt for 4 weeks elicited a significant cardioprotective activity by lowering the levels of cTnI, lipid profiles, and marker enzymes. The levels of LPO products were also significantly decreased. Elevated activities of antioxidant enzymes were also observed in ...
Methanolic extract ofAndrographispaniculata was evaluated for hepatoprotective and antioxidant activities in albino rats. The plant extract (250 and 500 mg/kg, bw.) exhibited a remarkable hepatoprotective and antioxidant activity againstparacetamol induced hepatotoxicity as judged from the serum marker enzymes and antioxidantlevels in liver tissues. Paracetamol induced a significant rise in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), lipid peroxidase(LPO) and bilirubinwith a reduction of protein, superoxide dismutase (SOD). Treatment of rats with different doses of plant extract(250 and 500 mg/kg) significantly (P<0.001) alteredserum marker enzymes and antioxidant levels to near normal againstparacetamol induce albino rats. The activity of the extract at dose of 250 mg/kg was comparable to more effective than dose of 500, mg/kgbw.). Histopathological changes of liver sample were compared with respective control. Results indicate the hepatoprotective and antioxidant properties of Andrographispaniculataagainstparacetamol -induced hepatotoxicity in albino rats.