Sample Preparation of Beta-Agonists in Urine, Comparison of Two Different Techniques; Mixed Mode Solid Phase Extraction and Molecular Imprinted Polymers Extraction (original) (raw)

Abstract

MIPs are engineered cross-linked polymers that can exhibit high affinity and selectivity towards specific compounds, e.g. beta-agonists. The interactions between MIP particles and analytes are stronger than interactions obtained in conventional SPE. Bond Elut Certify columns show a bi-model non-polar and strong cation exchange and are generally used for basic and neutral drugs in urine and other biological samples. Both types of clean up methods were optimized for more than 30 different beta-agonists. After optimization, both methods were fully validated according to Commission Decision 2002/657/EC. Analysis is carried out by LC-MSMS, Ultima Triple Quad system. Results indicate that both sample preparation methods can be used for a range of beta- agonists. However, none of these methods can be used for all compounds, e.g. nine compounds cannot be extracted with either method in the selected concentration range (1-5 μg L-1). Sample preparation using MIP columns gives generally cleane...

Peter Kootstra hasn't uploaded this paper.

Let Peter know you want this paper to be uploaded.

Ask for this paper to be uploaded.