Characterization of sunflower seed and kernel proteins (original) (raw)
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Characterization of Sunflower Seed Proteins by Electrophoretic Techniques
2000
The seed proteins of sunflower (Helianthus annuus) were qualitatively and quantitatively investigated. Qualitative studies were carried out using different electrophoretic techniques (SDS-PAGE, Poro-PAGE, 2-D SOS-PAGE, lsoelectric focusing, Mapping gels). Analysis of the water extracted flour on SOS-PAGE and SDS-Poro-PAGE gave five major polypeptides with MWs of 63.5 KD, 60 KO, 58 KD, 55 KO and 51 KO. The pattern of
Genetika, 2017
The most important criterion for introducing new confectionary sunflower hybrids into production is high protein yield. In the breeding process it is important to identify traits which could be used as selection criteria for increased kernel protein content. Increase of kernel protein content results in increased protein yield. This research was conducted during three vegetation seasons on 22 NS high-protein two-line confectionary sunflower hybrids produced within the breeding program at IFVCNS, Novi Sad, Serbia. Strong and very strong correlations were found among the largest number of examined traits. Based on the analysis of simple correlation coefficients, strong negative correlation was determined between kernel protein content and kernel ratio (-0.516*). A weak negative interdependence was determined between head diameter, seed protein content, and kernel protein content. Positive but weak correlation was determined between kernel protein content and thickness of seed, length ...
Heterogeneity and Polymorphism of Sunflower Seed 2S Albumins
Biotechnology & Biotechnological Equipment
2S albumin fractions were extracted from seven lines and two hybrid varieties of Heliantlm.~ amm1u ofwide~v different origins. Ana~v-~es by one-and two-dimensional sodium d()(/ecylsulphate po(vac1ilamide gel electrophoresis and by r(rversed phase high peifomwnc(! liquid chromatography showed that these fmction' were mixtures of po~vpeptides with M; bern•em 10 000 and 18 000. In addition, the nine genotypes differed in the presence or absence qf ce11ain po(vpeptides and in their relative abundances.
Sequence and expression of a gene encoding an albumin storage protein in sunflower
MGG Molecular & General Genetics, 1987
The complete sequence of a sunflower (Helianthus annuus) gene, HAG5, encoding a 2 S albumin storage protein was determined. The predicted unprocessed precursor has 295 amino acids, is rich in glutamine residues (24%) and contains a hydrophobic amino-terminus that is similar to the consensus signal peptide. Amino acid sequencing of the mature protein revealed extensive post-translational processing. Nuclease protection and primer extension analysis indicated a major transcriptional start 30 nucleotides 5' of the predicted ATG start codon. Additional sequence data, determined from a nearly full length cDNA recombinant, indicate that HaG5 is a member of a small gene family comprised of at least two divergent genes. Comparison of the predicted HaG5 gene product with sequences of other known plant proteins revealed distant but significant homology with the napins of Brassica and other heterogeneous seed proteins in the albumin superfamily.
Pakistan Journal of Agricultural Research, 2014
total proteins, oil content and fatty acids composition. Among parental lines, CMS-64, CMS-53, CMS-H55-2-2-1 and CMS-53 were the best general combiners for seed total proteins, oil content, oleic and linoleic acid, respectively. Among males, C-206-R, SF-187R, RHA-295 and RHA-854 were the potential parents exhibiting desirable GCA for seed total proteins, oil content, oleic and linoleic acids, respectively. Among the F hybrids, 1