Anti-Obesity Effects of Pulicaria Jaubertii E. Gamal-Eldin in High Fat Diet-Induced Rats (original) (raw)
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Evidence-Based Complementary and Alternative Medicine, 2012
Kudzu, a leguminous plant, has long been used in folk medicine. In particular, its flowers are used in Japanese and Chinese folk medicine for treating hangovers. We focused on the flower of Kudzu (Puerariae thomsonii), and we previously reported the antiobesity effect ofPuerariae thomsoniiflower extract (PFE) in humans. In this study, we conducted an animal study to investigate the effect of PFE on visceral fat and hepatic lipid levels in mice with diet-induced obesity. In addition, we focused on gene expression profiles to investigate the antiobesity mechanism of PFE. Male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with 5% PFE for 14 days. PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight. Moreover, in the histological analysis, PFE supplementation improved fatty liver. Hepatic reverse transcription-polymerase chain reaction revealed that PFE supplementation downregulated acetyl-CoA carboxylase expression. For adipo...
Preliminary Research for the Anti-obesity Effect of Puerariae Flos Extract in Humans
JOURNAL OF HEALTH SCIENCE, 2011
This research is preliminary study to examine whether puerariae flos extract (PFE) makes any action to body fat of man or not. We conducted the double-blind placebo controlled study on eighty mildly obese subjects for 8 weeks. The subjects were randomly divided into 4 groups: I, II, III, and IV. Subjects in groups I, II, and III consumed test food containing 100, 200, and 300 mg PFE, respectively, while those in group IV were given placebo food for 8 weeks. All subjects were instructed to restrict their total-energy intake to within 2650 kcal/day in the case of males and 2300 kcal/day in the case of females during the test period. Haematological and biochemical markers of blood, urinary markers, and physical markers were examined at 0, 4, and 8 weeks during the test period. As a result, no adverse effects were noted in any of the groups. For physical examinations, we analysed only males whose initial body mass index (BMI) values were over 24. BMI value and body weights of the subjects in group III were significantly reduced, and total fat area and subcutaneous fat area of the subjects in groups II and III were significantly reduced over the 8-week test period. Moreover, as a result of comparison among groups by unpaired t-test, significant difference appeared between group III and group IV in BMI. Total fat area in group III tend to decrease compared with group IV. By these results, the reduction of BMI in group III may reflect fat area reduction. To confirm the anti-obesity effect of PFE, we need to research particularly by extensive studies hereafter.
Bioscience, Biotechnology, and Biochemistry, 2012
In Japan, kudzu is a familiar plant, well-known as an ingredient in the Japanese-style confections kudzu-kiri and kudzu-mochi. In this study, we focused on the flower of kudzu (Pueraria thomsonii) and conducted a clinical trial to investigate the effects of Pueraria thomsonii flower extract (PFE) on obesity using obese Japanese males and females (BMI 25 kg/m 2). Eightyone obese subjects were randomly divided into three groups and consumed test food containing 300 mg of PFE, 200 mg of PFE, and a placebo over 12 weeks. The results indicate that PFE intake reduces BMI and decreases, the visceral fat area, but not the subcutaneous fat area. In addition, the decrease in visceral fat area showed no sexual dimorphism. Consequently, we propose that PFE intake expresses its BMI reduction effects via a decrease in visceral fat area.
Levels of obesity in Middle Eastern countries are increasing. Phytochemicals have anti-obesogenic properties as evidenced by prevention of adipocyte differentiation and blocking triacylglyceride (TG) accumulation. In Yemen, Pulicaria jaubertii E. Gamal-Eldin (PJ) is a food additive and a traditional medicine. We tested the hypothesis that phytochemicals present in PJ inhibit adipocytic responses during differentiation of 3T3-L1 preadipocytes to adipocytes. Methanolic extracts of PJ did not block expression of fatty acid binding protein 4 (FABP4) a marker of differentiation but did inhibit TG accumulation. Treatment of 3T3-L1 preadipocytes increased NADPH:quinone oxidoreductase 1 (NQO1), a suppressor of TG accumulation. Further fractionation of the methanolic PJ extract with hexane and dichloromethane (DCM) demonstrated that bioactivity towards TG reduction and elevated expression of NQO1 and other antioxidant genes (glutamate cysteine ligase catalytic unit, glutathione disulfide reductase, glutathione peroxidase (GPx) 4 resided in the DCM fraction. Activity towards depleting GSH and elevating the expression of catalase and GPx3 were found in the DCM and hexane fractions. Analysis by gas chromatography and liquid chromatography coupled with mass spectrometry demonstrated the presence of catechin-like moieties in the DCM and methanolic fractions and suggest that these components were partially responsible for the bioactivity of these fractions. In summary, our data indicate that fractions derived PJ exhibit anti-adipogenic properties in part through the presence of catechin-like compounds.
Advances in Bioscience and Biotechnology, 2014
Obesity is well recognized as a global public health concern and the quest continues to explore natural product-based novel therapeutics. Bauhinia purpurea finds its place in traditional medicine as an effective anti-ulcer, antiplatelet, wound healing and hypolipidemic agent. The present study investigated the effects of ethanolic extract of Bauhinia purpurea (EEB) on the changes in body weight, lean mass, fat free mass, fat percent, tissue and plasma lipid profiles, plasma glucose, insulin and insulin resistance as well as on the activity of amylase, lipase, leptin and adiponectin levels in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD, rats were administered orally with EEB (100, 200 and 300 mg•kg −1 BW) or orlistat (5 mg•kg −1 BW) once daily for 42 days. HCD substantially increased the body weight, fat free mass, fat percent, glucose, insulin resistance, tissue and plasma lipid profiles (Except HDL), leptin and the activities of amylase and lipase. EEB at a dose of 300 mg•kg −1 BW exerted its therapeutic effects similar to orlistat in decreasing body weight, insulin resistance, levels of glucose, leptin, plasma and tissue lipids, lipase and amylase but increasing HDL and adiponectin in HCD fed obese rats, which could be due to bioactive factors present in EEB, as revealed by LC-MS analysis. These findings suggest that EEB administration suppresses high-caloric-diet-induced obesity and it can be developed as a potential candidate for the treatment of obesity and associated complications.
Advances in Bioscience and Biotechnology, 2014
Obesity is well recognized as a global public health concern and the quest continues to explore natural product-based novel therapeutics. Bauhinia purpurea finds its place in traditional medicine as an effective anti-ulcer, antiplatelet, wound healing and hypolipidemic agent. The present study investigated the effects of ethanolic extract of Bauhinia purpurea (EEB) on the changes in body weight, lean mass, fat free mass, fat percent, tissue and plasma lipid profiles, plasma glucose, insulin and insulin resistance as well as on the activity of amylase, lipase, leptin and adiponectin levels in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD, rats were administered orally with EEB (100, 200 and 300 mg•kg −1 BW) or orlistat (5 mg•kg −1 BW) once daily for 42 days. HCD substantially increased the body weight, fat free mass, fat percent, glucose, insulin resistance, tissue and plasma lipid profiles (Except HDL), leptin and the activities of amylase and lipase. EEB at a dose of 300 mg•kg −1 BW exerted its therapeutic effects similar to orlistat in decreasing body weight, insulin resistance, levels of glucose, leptin, plasma and tissue lipids, lipase and amylase but increasing HDL and adiponectin in HCD fed obese rats, which could be due to bioactive factors present in EEB, as revealed by LC-MS analysis. These findings suggest that EEB administration suppresses high-caloric-diet-induced obesity and it can be developed as a potential candidate for the treatment of obesity and associated complications.
Global journal of health science, 2012
Puerariae flower extract (PFE) is a crude extract of the Kudzu flower. Previous studies have shown that PFE supplementation exerts anti-obesity and anti-fatty liver effects in high-fat diet-fed mice. In this study, we aimed to identify the PFE components responsible for these effects and to determine their influence on energy expenditure and uncoupling protein 1 (UCP1) expression. Experiments were conducted on C57BL/6J male mice classified into 3 groups: (1) high-fat diet-fed (HFD), (2) high-fat diet-fed given PFE (HFD + PFE), and (3) high-fat diet-fed given the PFE isoflavone-rich fraction (HFD + ISOF). All groups were fed for 42 days. The HFD + PFE and HFD + ISOF groups showed significant resistance to increases in body weight, hepatic triglyceride level, and visceral fat compared to the HFD group. These groups also exhibited significant increases in oxygen consumption and UCP1-positive brown adipose tissue (BAT) area. Our results demonstrate that the active ingredients in PFE are...
Puerariae lobatae root extracts and the regulation of brown fat activity
Phytomedicine, 2019
Background: Obesity is one of the major health problems worldwide. The induction of brown adipocyte formation and activity represents a promising therapeutic option by increasing energy expenditure. Asian herbs have the potential to treat obesity, however, pharmacological effects should be well documented at the molecular level first. Hypothesis: A novel hypothesis-driven screening approach identified the root of Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep (PLR) to have potential effects on obesity by stimulating brown adipocytes. Study design: This study explored the metabolic effects of PLR water extract (PLRE) in a high-fat diet-induced obesity mouse model and characterized its secondary metabolite composition. Methods: Animals were orally treated daily for two weeks and the bioactivity of PLRE evaluated by measuring various parameters including body weight, circulating metabolites, energy expenditure and insulin sensitivity. The chemical composition of the mains components was obtained by HPLC-MS-ELSD-PDA. Based on the dereplication results and semi-quantitative estimation, pure molecules were selected for tests on adipocytes in vitro. Results: PLRE induces brown adipocyte activity and triggers the formation of brown-like cells in inguinal fat tissue, weight loss, and improved glucose metabolism. These effects are primarily caused by cell-autonomous activation of brown adipocytes and not by autonomic nervous system regulation. Even though the analysis of PLRE revealed puerarin as the most abundant secondary metabolite, it showed no effect on brown adipocyte formation and function. Brown adipocyte activity was induced dose-dependently by two other isoflavones, daidzein, and genistein. Daidzein is present in a very small amount in PLRE, but various glycosidic isoflavones, including puerarin, may release daidzein after metabolism. Conclusion: This approach demonstrated the positive effects of PLRE on a diet-induced obesity mouse model and provided clues on the mode of action of PLRE at the molecular level.
Obesity is well recognized as a global public health concern and the quest continues to explore natural product-based novel therapeutics. Bauhinia purpurea finds its place in traditional medicine as an effective anti-ulcer, antiplatelet, wound healing and hypolipidemic agent. The present study investigated the effects of ethanolic extract of Bauhinia purpurea (EEB) on the changes in body weight, lean mass, fat free mass, fat percent, tissue and plasma lipid profiles, plasma glucose, insulin and insulin resistance as well as on the activity of amylase, lipase, leptin and adiponectin levels in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD, rats were administered orally with EEB (100, 200 and 300 mg·kg-1 BW) or orlistat (5 mg·kg-1 BW) once daily for 42 days. HCD substantially increased the body weight, fat free mass, fat percent, glucose, insulin resistance, tissue and plasma lipid profiles (Except HDL), leptin and the activities of amylase and lipase. EEB at a dose of 300 mg·kg-1 BW exerted its therapeutic effects similar to orlistat in decreasing body weight, insulin resistance, levels of glucose, leptin, plasma and tissue lipids, lipase and amylase but increasing HDL and adiponectin in HCD fed obese rats, which could be due to bioactive factors present in EEB, as revealed by LC-MS analysis. These findings suggest that EEB administration suppresses high-caloric-diet-induced obesity and it can be developed as a potential candidate for the treatment of obesity and associated complications.
2018
The aim of this study was to determine the effect of herbal product (HP) on the body weight reduction and lipid profile improvement in high fat diet (HFD)-induced obese Sprague Dawley (SD) rats. The ethyl acetate fraction (EAF) showed the highest total phenolic content (TPC) and total flavonoid content (TFC) at 30.48 ± 0.62 mg GAE/g and 156.52 ± 3.13 mg QE/g, respectively. It also showed the highest antioxidant capabilities to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) at IC 50 = 0.03 ± 0.00 mg/mL, nitric oxide (NO) at IC 50 = 1.00 ± 0.05 mg/mL and superoxide at IC 50 = 0.16 ± 0.01 mg/mL and reduce ferric ions at 109.27 ± 3.35 μmol Fe 2+ /g. However, the EAF showed low capability in chelating metal (< 50%). The oral acute toxicity test of the HP extract at a single dose of 5 g/kg body weight of rat showed no mortality and no toxic effect on SD rats. The body weight of HFD rats after two weeks treatment with water extract (WE), simvastatin and EAF was significantly reduced by 4.79%, 6.18% and 6.64%, respectively (p < 0.05). The body mass index (BMI) of the HFD rats after two weeks treatment with WE, simvastatin and EAF was also significantly reduced (p < 0.05). The treatment of HFD rats with EAF also led to significant reductions on triglyceride (TG), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C), with a positive elevation of high density lipoprotein cholesterol (HDL-C) compared to WE and simvastatin. In conclusion, the results showed that the EAF had better potential for body weight reduction and lipid profile improvement in HFD rats than WE and simvastatin.