Peer Reviewed: Analytical Bioluminescence and Chemiluminescence (original) (raw)

Bio- and chemiluminescence in bioanalysis

Fresenius' Journal of Analytical Chemistry, 2000

Analytical chemiluminescence and bioluminescence represent a versatile, ultrasensitive tool with a wide range of applications in diverse fields such as biotechnology, pharmacology, molecular biology, clinical and environmental chemistry. Enzyme activities and enzyme substrates and inhibitors can be efficiently determined when directly involved in luminescent reactions, and also when they take part in a reaction suitable for coupling to a final light-emitting reaction. Chemiluminescence detection has been exploited in the fields of flow-injection analysis and column-liquid chromatographic and capillary-electrophoretic separative systems, due to its high sensitivity when compared with colorimetric detection. It has widely been used as an indicator of reactive oxygen species formation in cells and whole organs, thus allowing the study of a number of pathophysiological conditions related to oxidative stress. Chemiluminescence represents a sensitive and rapid alternative to radioactivity as a detection principle in immunoassays for the determination of a wide range of molecules (hormones, food additives, environmental pollutants) and in filter membrane biospecific reactions (Southern, Northern, Western, dot blot) for the determination of nucleic acids and proteins. Chemiluminescence has also been used for the sensitive and specific localization and quantitation of target analytes in tissue sections and single cells by immunohistochemistry and in situ hybridization techniques. A relatively recent application regards the use of luminescent reporter genes for the development of bioassays based on genetically engineered microorganisms or mammalian cells able to emit visible light in response to specific inorganic and organic compounds. Finally, the high detectability and rapidity of bio-and chemiluminescent detection make it suitable for the development of microarraybased high throughput screening assays, in which simultaneous, multianalyte detection is performed on multiple samples.

Fluorescence, Phosphorescence, and Chemiluminescence

Analytical chemistry, 2015

Fluorescence, Phosphorescence, and Chemiluminescence cover a wide array of topics. One can simply examine the number of topics that arise from photon induced excitation and emission as summarized in Figure 1 to understand the magnitude of this overall topic. Thus, as in previous years, we are not able to provide extensive coverage of all developments of relevance to this extremely broad area of research. Instead, we have attempted to focus on important advances of general interest and relevance to the field of analytical chemistry. In addition, rather than cover extensions of previous advances, we have attempted to balance inclusion of a sufficient number of highly relevant, high-impact references to adequately survey the field with ample descriptions of individual citations for better clarification. If you feel that we have omitted an important article published during the above referenced time period, please forward the reference to the journal for inclusion in the next review.