The effect of autohydrolysis on the production of second generation bioethanol from extracted olive pomace (original) (raw)

Purpose: To study the effect of autohydrolysis pretreatment in the use of extracted olive pomace (EOP) for bioethanol production after enzymatic hydrolysis in a Separate Hydrolysis and Fermentation (SHF) approach. Methods: EOP treated by autohydrolysis under different conditions was characterised and their enzymatic hydrolysis with Celluclast 1,5L was accessed by studying enzyme concentration and hydrolysis kinetics. After enzymatic hydrolysis, batch fermentations were carried out using Saccharomyces cerevisiae NCYC-1119 under SHF conditions. Results: The operational conditions of autohydrolysis have a marked influence on the enzymatic digestibility, enabling an increase of almost 10 fold from the milder, to the optimal conditions found, for which a glucose yield of 83% was obtained. The extension of the enzymatic hydrolysis is discussed and modeled based on the macromolecular composition of the pre-treated EOP. Fermentation presented a yield of 76.5% of ethanol, when compared to YP...