Purification and Partial Characterization of English Sole ( Pleuronectes vetulus) Vitellogenin (original) (raw)

Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology, 1997

Abstract

Vitellogenin (VTG) was purified by double-step chromatography from plasma of male English sole treated with 17β-estradiol. The intact protein appeared to exist as a dimer in two forms of approximately 300 and 320 kDa and had an isoelectric point of 6.63. In SDS-PAGE, it was reduced to a single monomer of approximately 130 kDa. In immunoblotting, the protein showed cross-reactivity

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