Inactivated Vaccine Trial of Mycoplasma gallisepticum in Ethiopia (original) (raw)
Related papers
Zenodo (CERN European Organization for Nuclear Research), 2022
The present study monitored the effect of Mycoplasma gallisepticum infection on the humeral immune response of chickens against the different inactivated bivalent vaccines (ND, AI), (ND, IB), (ND, IBD) and monovalent (Reo Vaccine). These inactivated vaccines were used to inoculate three hundred 3-week-old specific pathogenfree (SPF) chicks by S/C route. They were grouped into nine groups; Groups (1, 3, 5 and 7) pre-infected with MG one week before vaccination and Groups (2,4, 6 and 8)vaccinated and non-infected. Group (9) was kept as control. In this study field infection of Mycoplasma gallisepticum was designed by inoculation of 250uL of the infectious material suspension containing 10 6 cfu of MG was instilled in the nasal sinuses as well as injected subcutaneously one week before vaccination. The immune response was estimated and evaluated by using HI, ELISA and challenge tests. The bacterial stress of M. gallisepticum was recorded by the lesion scores. The results of HI and ELISA tests for the pre-infected groups showed highest antibody titers against (ND, AI),
Antibodies response of broilers to locally prepared oil based Mycoplasma gallisepticum vaccine
Journal of Animal and Plant Sciences
In the present study formaldehyde inactivated Montanide ISA70 based MG vaccine from the PCR confirmed positive local isolate was prepared and evaluated in broilers. An amount of immune gen per 0.3ml of the dose was 10 7 Colony forming units of the bacteria. At the age of 14 days, the broilers were randomly divided into three groups (A, B and C), each having twenty birds. Each bird of group A, B and C was inoculated with 0.3 ml of sterile Frey's broth (negative control), indigenous vaccine (IN-VAC) and imported (IM-VAC; VaxFact-USA) subcutaneously at mid neck region, respectively. The birds of group A (Control group) showed 28.
Thai Journal of Veterinary Medicine, 2006
This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were tested for MG Infection by culture and polymerase chain reaction (PCR) procedures. Sera were determined for MG antibody responses by a serum plate agglutination (SPA) test and by 2 commercial enzyme-linked immunosorbant assay (ELISA) test kits, IDEXX®and Synbiotics®. Results revealed that MG organisms were only found in groups 3 and 4 using culture and isolation when 5 and 11 weeks old,...
Mycoplasma is unique among prokaryotes because of its small size, small genomes, and complete lack of cell walls, which makes them cell wall-less prokaryotes. This study aimed to evaluate the effect of vaccinating oneday-old chicks with inactivated and live vaccines (CRDF) of Mycoplasma gallisepticum (MG) on their humoral immune response and immune organs. The Enzyme-Linked Immunosorbent Assay was used to measure Ab titers and investigate histopathological changes. A total of 130 one-day-old broiler chicks were randomly divided into four groups of 30. The groups were treated as follows: G1 included the chicks vaccinated with live F-strain MG vaccine (on eye drop of 0.03ml/dose), G2 included the chicks vaccinated with inactivated MG (0.3 ml s.c) vaccine, G3 included the chicks vaccinated with inactivated and live MG vaccines, and G4 was considered the control group, in which the chicks were not vaccinated. Blood samples were collected on days 21 and 35 of the chick's life to measure the titers of specific antibodies. On day 35, the chicks were dissected, and the bursa of Fabricius, as well as the spleen, were removed for histological evaluations. On day 21, the results showed a significant difference (P≤0.05) between all vaccinated groups in Ab titers, compared to G4, with the highest mean in G3, followed by G2 and G1, in descending order. On day 35, there was a significant difference (P≤0.05) between G3 and other vaccinated groups (G2 and G1), as well as G4. In addition, there was a significant increase in all vaccinated groups on day 35, compared to day 21. In G1, histopathological examination results showed a moderate lymphocytic hyperplasia bursal follicle. In G2, varying degrees of lymphoproliferative were observed in the major bursal follicle, and in G3, a marked lymphocytic hyperplasia bursal follicle was observed. In G4, on the other hand, no obvious histopathological findings were recorded. The results of the spleen histopathological evaluation showed various degrees of lymphoproliferative and moderate neutrophilic infiltrate in the red pulp in G1, and mild sinus congestion with scattered lymphocytes was recorded in the lumen in G2. In the spleen of the chicks in G3, reactive lymphoid hyperplasia was observed. In contrast to the groups mentioned above, in G4, the spleen structure showed a typical structure. It was concluded that the chicks vaccinated with inactivated and live MG vaccines experienced increased production of Ab titers and the immune stimulation of immune organs.
Asian Journal of Pharmaceutical and Clinical Research, 2016
ABSTRACTObjective: The study of Mycoplasma gallisepticum (MG) infection is needed, not only to understand the disease process but also to understand theinterference with the evaluation of some live viral poultry vaccines. This study aims to investigate the titration and potency of some live attenuatedpoultry viral vaccines; Newcastle disease, infectious bronchitis, infectious bursal disease, and Reo in both specific pathogen-free (SPF) embryonatedchicken eggs (ECEs) and chickens.Methods: Titration of live attenuated viral poultry vaccines in ECEs was carried out by dividing the inoculated eggs into four groups; the pre-,simultaneously-, post-, and non-MG contaminated. MG effect on the potency test was carried out using seventeen groups of SPF chickens (25 chicken/group) placed into separate isolators. Each live attenuated viral poultry vaccine was inoculated into 4 groups.Results: The highest titer of these vaccines that appeared in MG pre- contaminated ECEs were 1011, 107.5, 107.9,...
Veterinary Microbiology, 2021
Immunosuppression can increase the susceptibility of chickens to other disease-causing pathogens and interfere with the efficacy of vaccination against those pathogens. Chicken anaemia virus (CAV) and infectious bursal disease virus (IBDV) are common causes of immunosuppression in chickens. Immunosuppression was induced by experimental infection with either CAV or IBDV to assess the effect of immunosuppression on the efficacy of Mycoplasma gallisepticum vaccine strain ts-304 against infection with virulent M. gallisepticum, a common bacterial pathogen of chickens worldwide. Birds were experimentally infected with either CAV or IBDV at 1 week of age, before vaccination and challenge with M. gallisepticum to examine the effect of immunosuppression at the time of vaccination, or at 6 weeks of age, after vaccination against M. gallisepticum but before challenge with virulent M. gallisepticum, to investigate the effect of immunosuppression at the time of challenge. All birds were vaccinated with a single dose of the ts-304 vaccine at 3 weeks of age and experimentally challenged with the virulent M. gallisepticum strain Ap3AS at 8 weeks of age. In immunosuppressed chickens there was a reduction in protection offered by the ts-304 vaccine at two weeks after challenge, as measured by tracheal mucosal thicknesses, serum antibody levels against M. gallisepticum, air sac lesion scores and virulent M. gallisepticum load in the trachea. Immunosuppressed birds with detectable serum antibodies against M. gallisepticum were less likely to have tracheal lesions. This study has shown that immunosuppression caused by infection with CAV or IBDV can interfere with vaccination against mycoplasmosis in chickens. Key words: chicken, Mycoplasma gallisepticum, vaccine, immunosuppression, chicken anaemia virus, infectious bursal disease virus * Unchallenged. All the groups were negative for anti-M. gallisepticum antibodies by RSA before vaccination. Values marked with the same superscript letter in the same column are not significantly different (P > 0.05).
2016
Received: Revised: Accepted: Published online: October 20, 2014 August 18, 2015 September 01, 2015 December 31, 2015 To inactivate locally isolated Mycoplasma gallisepticum (MG), phenol, formaldehyde and binary ethylenimine were optimized for their concentrations and exposure time. Their effect on immunoprophylaxis induction was then assessed by using different levels of immunogen/PCV and different adjuvants. 0.10M, 0.125% and 0.6% concentrations of binary ethylenimine, formaldehyde and phenol inactivated MG colonies within 24, 8 and 4 hours respectively. Formaldehyde inactivated vaccine with 1.5% Immunogen/PCV and montanide oil as adjuvant induced significantly (P<0.05) higher anti MG antibody titer than other locally prepared vaccines. Conclusion is that montanide oil adjuvated indigenous formalized bacterin can induce desired immune response. ©2015 PVJ. All rights reserved
2015
Aim: Mycoplasma gallisepticum (MG) is important avian pathogen responsible for chronic respiratory disease of chicken and turkeys, which result in large economic loss for the poultry industry. The objectives of this study were determination of seroprevalence of MG antibody of commercial layer chicken at laying period in selected areas of Bangladesh. Materials and Methods: A total of 563 blood samples were collected randomly from selected commercial layer chickens at laying period during the period from July to December, 2013. Indirect enzyme linked immunosorbent assay (iELISA) and serum plate agglutination (SPA) test were performed to detect the presence of antibodies against MG. Results: Of 563 samples, 64.47% and 56.13% showed an overall prevalence of MG antibodies in iELISA and SPA test respectively. Prevalence of MG was recorded the highest (69.63%) at 50-55 weeks of age compared with lowest (53.26%) at 56-61 weeks of age (p<0.05). Significant (p<0.05) effect of breed were observed in the seroprevalence of MG infection in layer birds in the present study. The overall, 68.77%, 63.74% and 59.37% prevalence were found respectively in sonali, ISA Brown and White leg horn. The prevalence of MG antibodies was the highest (70.13%) in December followed by November (68%), October (65.67%), August (63.46%), September (58.54%) and July (51.78%) month. The seroprevalence of MG antibodies was higher (69.63%) in most of the large flocks and lower (56.82%) in small flocks. Conclusion: Therefore, might be suggested that the commercial layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically. The correlation between MG antibody in month and flock size was not significant (p=0.359 and p=0.868, respectively).
Fawzy. R. El Seedy1 , S.M.Tamam2, Hala Sayed Hassan1, and Mona Gamal Eldeen mohamed1 1 Department of Bacteriology. Mycology and immunology, Faculty of Vet.Med. Beni suef University. 2 Department of virology, Faculty of Vet.Med.,Bani suef University., 2018
The experiment was designed to investigate the immunomodulating effect of lector 50 on general health and immune response of broiler chicks to Mycoplasma gallisepticum vaccination in commercial broiler chicks. the obtained results reveled significantly higher effects on body weight ,bursal, and thymic index on lector treated group of chickens, while no effects on spleen index. Also significant improvement in total and differential leukocytic count as well as significantly higher antibody titer was detected by ELISA in lector 50 treated groups.