Technical viability of the production of sweet potato plantlets in trays Viabilidade técnica da produção de mudas de batata-doce em bandejas (original) (raw)
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An experiment was carried out from December 2005 to July 2006, in the Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), in Diamantina, Minas Gerais State, Brazil, aiming at selecting sweet potato clones for the Alto Vale do Jequitinhonha. We evaluated nine clones from the UFVJM germplasm bank, using cultivars Brazlândia Branca, Brazlândia Roxa, and Princesa as controls. The experimental design was blocks at random, with four replications. Plants were harvested seven months after transplanting. We assessed the fresh mass yield of vines and roots, as well as root shape and resistance to soil insects. Genotypes did not differ from each other for the fresh mass yield of vines (ranging from 3.81 to 11.76 t ha-1). The total yield of roots ranged from 22.0 to 45.4 t ha-1 and clones BD-06, BD-113-TO, BD-15, BD-38, BD-25, BD-61, and cultivar Princesa had statistically the highest figures. However, only clone BD-06 significantly overcame the control cultivars Brazlândia Branca...
Technical viability of the production of sweet potatoes plantlets in trays
Semina: Ciências Agrárias, 2011
The cultivation of sweet potato requires high amount of stems per area unit and the rooting of minicuttings can contribute to the production of large number of plants in a short time. Thus, this study aimed to investigate the technical viability of plantlets production of sweet potato in trays. The treatments consisted of mini-cuttings (segments with two nodes removed from the middle and upper thirds of branches) of 30 clones of sweet potato which were grown for 35 days in trays and then transplanted to a permanent site. The experimental design was completely randomized with four replications. The following characteristics were evaluated: percentage of rooting and survival of plantlets after transplanting. The average mini-rooted cuttings was approximately 95%. The survival rate of the clones in the field was above 98%. It was concluded that it is technically viable the production of sweet potato in trays.
Desempenho de clones de batata-doce para a produção de bioetanol em função de períodos de cultivo
Horticultura Brasileira, 2017
A batata-doce [ Ipomoea batatas (L.) Lam.] e tecnicamente uma planta perene, plantada e explorada como anual. Sendo assim, a identificacao do periodo de cultivo e de clones de batata-doce e importante para um melhor aproveitamento dessa cultura, tanto na alimentacao humana e animal, como para producao de etanol. O objetivou-se com esse trabalho avaliar o desempenho de clones de batata-doce cultivados em diferentes periodos de cultivo de cultivo. Foram testados nas parcelas tres periodos de cultivo de cultivo (3, 5 e 7 meses), e nas subparcelas, cinco clones de batata-doce (acessos IBP-007, IBP-038, IBP-075, IBP-079 e IBP-149), e a cultivar Brazlândia Rosada. As variaveis analisadas foram: teor de materia seca de raiz, amido e amilose e rendimento de amido e etanol. O clone IPB-007 apresentou elevados valores para rendimento de amido (6,63 t.ha -1 ) e etanol (4.379 L.ha -1 ). Ja para teor de materia seca de raiz (38,32%) e amido (26,70%) e rendimento de etanol (176,26 em L.t -1 ), o ...
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2011 SEMINA-Technical viability of the production
The cultivation of sweet potato requires high amount of stems per area unit and the rooting of minicuttings can contribute to the production of large number of plants in a short time. Thus, this study aimed to investigate the technical viability of plantlets production of sweet potato in trays. The treatments consisted of mini-cuttings (segments with two nodes removed from the middle and upper thirds of branches) of 30 clones of sweet potato which were grown for 35 days in trays and then transplanted to a permanent site. The experimental design was completely randomized with four replications. The following characteristics were evaluated: percentage of rooting and survival of plantlets after transplanting. The average mini-rooted cuttings was approximately 95%. The survival rate of the clones in the field was above 98%. It was concluded that it is technically viable the production of sweet potato in trays.
In Vitro propagation of sweet potato (Ipomoea batatas (L.) Lam) through apical meristem culture
Sweet potato (Ipomoea batatas L.) is an important warm season crop that grows best in long, hot growing seasons, cultivated in over all developing countries as a valuable source of human food, animal feed and industrial raw material. Its yield is highly decreased due to disease in Eastern African Countries. Production of virus free sweet potato is of great potential to increase yield and improve the crop. In the present study, different treatments, each with a combination of (BAP and Kn) in a ratio 0.25, 0.5, 0.75, 1.0 and 0.25, 0.5, 0.5, 0.75 and 0.0 mg/l (control) and auxin (IBA and NAA) in a ratio 0, 0.5, 0.5, 1.0, 1.0mg/l and 0, 0.25, 0.5, 0.5 and 1.0mg/l in combination respectively were used for three varieties (Beletech, Awassa-83 and Belela) to optimize the concentrations of BAP, Kn, IBA and NAA in MS basal media for meristem culture. The experiment was laid out in CRD with three replications in factorial arrangement. Among the growth regulator combinations tested 0.5mg/l BAP with 0.5mg/l Kn followed by 0.75mg/l BAP with 0.5mg/l Kn were best for all shoot induction and growth parameters for the three varieties. The minimum days to root induction was recorded for Beletech (3.167days) shoots cultured on media supplemented with 0.5mg/l IBA with 0.5mg/l NAA and Awassa-83 (3.83days) shoots cultured on media supplemented with 1.0mg/l IBA and 0.5mg/l NAA. Whereas, the variety Belela shoots have induced in (3.83 days) on media with 1.0mg/l IBA with 1.0mg/l NAA. Maximum number of roots per shoot were recorded on MS media supplemented with a combination of 1.0mg/l IBA with 0.5mg/l NAA (11.7) followed by (9.3) on media with 0.75mg/l IBA with 0.5mg/l NAA respectively. The maximum root length was observed for Beletech (3.4cm) followed by Awassa-83 (3.43cm) cultured on the media with a combination of IBA 0.75mg/l with NAA 0.5mg/l. The results indicated that better response was obtained for root related parameters of all variety shoots transferred for rooting on media supplemented with 0.5mg/l IBA with 0.5mg/l NAA and above up to 1.0mg/l IBA with 0.5mg/l NAA. During acclimatization, 90%, for Beletech and 80% for Awassa-83 and Belela varieties survival was obtained.
In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars
Agricultural Science Digest - A Research Journal, 2018
The study is aimed at establishing a simple protocol for in vitro regeneration of sweet potato with a view to providing planting materials to farmers as well as basis for genetic improvement. Axillary buds were excised and cultured on Murashige and Skoog (MS) basal salts supplemented with 6-benzyl aminopurine (BAP), gibberellic acid (GA3) and naphthalene acetic acid (NAA) singly or in combination. The shoot height and number of leaves differed significantly among the cultivars. The result also indicated significant difference (p less than 0.01) among the cultivars with King J recording the highest mean values. Significant differences (p less than 0.05) was also recorded in the media combination with respect to organogenesis and number of shoots obtained. The results of hardening further revealed 33.33% success in the explants transferred directly to the field, as well as for the plantlets that were gradually weaned in a mixture of 3:1 sand and biochar.
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Regeneração de plantas de bananeira do grupo AAA a partir de calos cultivados durante longo período
Pesquisa Agropecuaria Brasileira, 1998
The banana plant is one of the most widely cultivated crops in the world. ilowever, banana breeding has been a slow process, dueto the Iow seed set and low germination rates. Selection ofuseful somaclonal variations and genetic transformation in edIs or cailuses are promising techniques to accelerate the breeding process. Therefore, cailus culture was carried out, aiming the establishment of one protocol for plant regencration, to be used in banana breeding prograin. Leaf sheath disks of 'Nanico' banana (Musa sp., AÁA group, Cavendish subgroup) were cultured on a Murashige and Skoog (MS) basal medium supplemented with activated charcoal (0.2 %), MES (2 [N-morpholino] ethanesulfonic acid) (15.3 mM), arginine (300 mM), Picloram (414 1sM) and 2iP (2-isopentenyl adenine) (492 uM). Globular calluses developed on the Ieaftissue were subcultured in the sarne medium, acquiring •a friable and translucid appearance after one and a half year of culture. The friable calluses were transferred to tbe medium without growth regulators and arginine, and supplemented with casem hydrolysate (0.05%), where they formed embiyo-like structures after transference to Iight. From these structures, shoots with roots were obtained and plantlets developed. The plant regeneration protocol shown here may be useful to banana breeding via somaclonal variation. Index terms: Musa, tissue culture REGENERAÇÃO DE PLANTAS DE BANANEIRA DO GRUPO AAA A PARTIR DE CALOS CULTIVADOS DURANTE LONGO PERÍODO RESUMO-A bananeira duma das plantas mais cultivadas no mundo. Porém, o melhoramento genético de bananeira tem sido um processo vagaroso, em virtude das baixas taxas de formação e germinação de sementes. São técnicas promissoras a seleção de variações somaclonais úteis e a transformação genética em células e calos, para acelerar o processo de melhoramento. Realizou-se a cultura de calos, com o objetivo de estabelecer um protocolo de regeneração de plantas, para ser usado no programa de melhoramento genético de bananeira. Discos de bainha foliar da banana cv. Nanicão (Musa sp., grupo AAA, subgrupo Cavendish) foram cultivados no meio básico de Murashige e Skoog (MS) suplementado com carvão ativado (0,2 0/*), MES (ácido 2 IN-morfolino] etanesulfônico) (15.3 mM). arginina (300 mM), Picloram (414 MM) e 2ip (2-isopentenil adenina) (492 MM). Calos globulares surgidos nos tecidos foliares foram subcultivados no mesmo meio, e obteve-se uma aparência friável e translúcida após um ano e meio de cultura. Os calos friáveis foram transferidos para meio sem reguladores de crescimento e arginina, e suplementado com caseina hidrolisada (0,05%), onde formaram estruturas semelhantes a embriões após transferência à luz. A partir destas estruturas, foram obtidos brotos com raizes, dos quais se originaram plántulas. O protocolo da regeneração de plantas apresentado aqui poderá ser útil para o melhoramento genético de bananeira via variação somaclonal. Termos para indexação: Musa, cultura de tecidos.