Fabrication of biosensors by attachment of biological macromolecules to electropolymerized conducting films (original) (raw)
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Biosensors and Bioelectronics, 1999
The concept and potentialities of electrochemical procedures of biomolecule immobilization based on electropolymerized films are described. The biomolecule entrapment in conventional electrogenerated polymers such as polypyrrole, polyaniline or polyphenol is compared with an electrochemical procedure involving the adsorption of amphiphilic monomers and biomolecules before the polymerization step. Examples of organic phase enzyme electrode and electrical wiring of immobilized enzymes are presented. Furthermore, the construction of controlled architectures based on spatially segregated multilayers, exhibiting complementary biological activities is described. Then, the use of functionalized polymers bearing functional groups for the covalent binding of biomolecules is reported. Moreover, the attachment of biomolecules to biotinylated polymers through affinity interactions based on avidin-biotin bridge is presented.
Biosensors Based on Immobilization of Biomolecules by Electrogenerated Polymer Films
Applied Biochemistry and Biotechnology, 2000
The concept and potentialities of electrochemical procedures of biomolecule immobilization are described. The entrapment of biomolecules within electropolymerized films consists of the application of an appropriate potential to an electrode soaked in an aqueous solution containing monomer and biomolecules. This method of biosensor construction is compared with a two-step procedure based on the adsorption of an aqueous amphiphilic pyrrole monomer-biomolecule mixture on an electrode followed by the electropolymerization of the adsorbed monomers. Another approach is based on the electrogeneration of polymer films functionalized by specific groups allowing subsequently the attachment of biomolecules. The immobilization of biomolecules on these films by covalent binding or noncovalent interactions is described.
Biomolecular immobilization on conducting polymers for biosensing applications
Biomaterials, 2007
A detail study on different aspects of biomolecule immobilization techniques on conducting polymers (CP) for applications in biosensors is described. Comparative studies are conducted in between the different mode of biomolecule immobilization techniques, viz. physical, covalent and electrochemical immobilization onto the conducting polymer films for the fabrication of electrochemical biosensors for clinical, food and environmental monitoring applications. This review focuses on the current status of biomolecule immobilization techniques on CP and their applications in the development of amperometric biosensors. r
Electrochemical Biosensors Based on Conducting Polymers
Ionic Carriers in Organic Electronic Materials and Devices, 2010
Conducting polymers are an important class of functional materials that has been widely applied to fabricate electrochemical biosensors, because of their interesting and tunable chemical, electrical, and structural properties. Conducting polymers can also be designed through chemical grafting of functional groups, nanostructured, or associated with other functional materials such as nanoparticles to provide tremendous improvements in sensitivity, selectivity, stability and reproducibility of the biosensor's response to a variety of bioanalytes. Such biosensors are expected to play a growing and significant role in delivering the diagnostic information and therapy monitoring since they have advantages including their low cost and low detection limit. Therefore, this article starts with the description of electroanalytical methods (potentiometry, amperometry, conductometry, voltammetry, impedometry) used in electrochemical biosensors, and continues with a review of the recent advances in the application of conducting polymers in the recognition of bioanalytes leading to the development of enzyme based biosensors, immunosensors, DNA biosensors, and whole-cell biosensors.
A review study of (bio)sensor systems based on conducting polymers
This review article concentrates on the electrochemical biosensor systems with conducting polymers. The area of electro-active polymers confined to different electrode surfaces has attracted great attention. Polymer modified carbon substrate electrodes can be designed through polymer screening to provide tremendous improvements in sensitivity, selectivity, stability and reproducibility of the electrode response to detect a variety of analytes. The electro-active films have been used to entrap different enzymes and/or proteins at the electrode surface, but without obvious loss of their bioactivity for the development of biosensors. Electropolymerization is a well-known technique used to immobilize biomaterials to the modified electrode surface. Polymers might be covalently bonding to enzymes or proteins; therefore, thickness, permeation and charge transport characteristics of the polymeric films can be easily and precisely controlled by modulating the electrochemical parameters for various electrochemical techniques, such as chronoamperometry, chronopotentiometry, cyclic voltammetry, and differential pulse voltammetry. This review article is divided into three main parts as given in the table of contents related to the immobilization process of some important conducting polymers, polypyrrole, polythiophene, poly(3,4-ethylenedioxythiophene), polycarbazole, polyaniline, polyphenol, poly(o-phenylenediamine), polyacetylene, polyfuran and their derivatives. A total of 216 references are cited in this review article.
Electropolymerization of amphiphilic monomers for designing amperometric biosensors
Electroanalysis, 1997
The principle and potentialities of an original electrochemical procedure of biosensor construction based on amphiphilic pyrrole derivatives are reviewed. The two-step procedure consists of the adsorption of an aqueous amphiphilic pyrrole monomer-biomolecule mixture on an electrode surface followed by the electrochemical polymerization of the adsorbed amphiphilic monomers. This method is compared with the more conventionally used electrochemical procedures of biosensor construction. Examples of multienzyme and additive entrapments, organic phase enzyme electrode, microelectrode functionalization and the electrical wiring of immobilized enzymes are presented.
Talanta, 1997
Electrodes modified by the electrodepozition of conducting organic polymers such as poly(3-methylthiophene)(PMT), polypyrrole (PPY) and polyaniline (PAN) were used as chemical sensors for voltammetric analysis and flow injection detection of some organic and biological molecules. The electrochemical behaviors of catechol, ascorbic acid, hydroquinone, dopamine, epinephrine, acetaminophen and p-aminophenol were examined by differential pulse voltammetry. The electrochemical behavior of these molecules at different electrodes was compared and the effects on behavior of electrolyte type and its pH and the film thickness were systematically examined. The results showed that the proposed modified surface catalyzes the oxidation of these compounds. Electrocatalytic 'efficiency' decreases in order of poly-3-methylthiophene, polypyrrole and polyaniline. Voltammetric peak positions were affected by the nature of the electrolyte and its pH. Also, the effect of increasing film thickness was to observe increased peak heights. Polymer coated electrodes were also used in an amperometric detector for flow injection analysis of most of the these compounds. The responses of the polymer electrode were 5-15 times larger as compared with those of bare platinum. PMT showed improved performance as an amperometric detector for flow injection analysis systems over other types of polymer electrodes. Detection limits as low as 10-8-10 9 M were achieved using the PMT, compared with 10 -6 10 8 M using platinum electrodes. In the flow injection analysis, with increasing molecular weight of analyte molecules was to observe decreased peak heights. © 1997 Elsevier Science B.V.
Biomimetic electrochemistry from conducting polymers. A review
Electrochimica Acta, 2012
Films of conducting polymers in the presence of electrolytes can be oxidized or reduced by the flow of anodic or cathodic currents. Ions and solvent are exchanged during a reaction for charge and osmotic pressure balance. A reactive conducting polymer contains ions and solvent. Such variation of composition during a reaction is reminiscent of the biological processes in cells. Along changes to the composition of the material during a reaction, there are also changes to other properties, including: volume (electrochemomechanical), colour (electrochromic), stored charge (electrical storage), porosity or permselectivity (electroporosity), stored chemicals, wettability and so on. Most of those properties mimic similar property changes in organs during their functioning. These properties are being exploited to develop biomimetic reactive and soft devices: artificial muscles and polymeric actuators; supercapacitors and all organic batteries; smart membranes; electron-ion transducers; nervous interfaces and artificial synapses, or drug delivery devices. In this review we focus on the state of the art for artificial muscles, smart membranes and electron-ion transducers. The reactive nature of those devices provide them with a unique advantage related to the present days technologies: any changes in the surrounding physical or chemical variable acting on the electrochemical reaction rate will be sensed by the device while working. Working under constant current (driving signal), the evolution of the device potential or the evolution of the consumed electrical energy (sensing signals) senses and quantifies the variable increment. Driving and sensing signals are present, simultaneously, in the same two connecting wires. It is possible to prepare electrochemical devices based on conducting polymers in which there are several kinds of different sensors and one actuator embedded in one device. Examples of the tools and products, start-up companies, increasing evolution of scientific literature and patents are also presented. Scientific and technological challenges are also considered.
Universal Method for Direct Bioconjugation of Electrode Surfaces by Fast Enzymatic Polymerization
Biosensors and Bioelectronics
We report HRP-catalyzed polymerization of Tannic acid (TA) and application of the poly (Tannic acid) (p(TA)) as a versatile platform for covalent immobilization of biomolecules on various electrode surfaces based on electrochemical oxidation of the p(TA) and subsequent oxidative coupling reactions with the biomolecules. We also used this method for capturing cancer cells through a linker molecule, folic acid (FA). Furthermore, we have demonstrated that enhanced electrocatalytic activity of the p(TA)-modified surface could be used for simultaneous electrochemical determination of biologically important electroactive molecules such as ascorbic acid (AA), dopamine (DA), and uric acid (UA). This HRP-catalyzed polymerization of TA and p(TA)-mediated surface modification method can provide a simple and new framework to construct multifunctional platforms for covalent attachment of biomolecules and development of sensitive electrochemical sensing devices