Spatial Systems Lipidomics Reveals Nonalcoholic Fatty Liver Disease Heterogeneity (original) (raw)
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Biomolecules, 2020
An imbalance between hepatic fatty acid uptake and removal results in ectopic fat accumulation, which leads to non-alcoholic fatty liver disease (NAFLD). The amount and type of accumulated triglycerides seem to play roles in NAFLD progression; however, a complete understanding of how triglycerides contribute to NAFLD evolution is lacking. Our aim was to evaluate triglyceride accumulation in NAFLD in a murine model and its associations with molecular mechanisms involved in liver damage and adipose tissue-liver cross talk by employing lipidomic and molecular imaging techniques. C57BL/6J mice fed a high-fat diet (HFD) for 12 weeks were used as a NAFLD model. Standard-diet (STD)-fed animals were used as controls. Standard liver pathology was assessed using conventional techniques. The liver lipidome was analyzed by liquid chromatography–mass spectrometry (LC–MS) and laser desorption/ionization–mass spectrometry (LDI–MS) tissue imaging. Liver triglycerides were identified by MS/MS. The t...
The Role of Fatty Acids in Non-Alcoholic Fatty Liver Disease Progression: An Update
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Non-alcoholic fatty liver disease (NAFLD) is a major public health problem worldwide. NAFLD (both simple steatosis and steatohepatitis) is characterized by alterations in hepatic lipid metabolism, which may lead to the development of severe liver complications including cirrhosis and hepatocellular carcinoma. Thus, an exhaustive examination of lipid disorders in the liver of NAFLD patients is much needed. Mass spectrometry-based lipidomics platforms allow for in-depth analysis of lipid alterations in a number of human diseases, including NAFLD. This review summarizes the current research on lipid alterations associated with NAFLD and related complications, with special emphasis on the changes in long-chain and short-chain fatty acids levels in both serum and liver tissue, as well as in the hepatic expression of genes encoding the enzymes catalyzing lipid interconversions.
Noninvasive hepatic lipid quantification with magnetic resonance imaging and spectroscopy
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Excessive accumulation of intra-hepatocellular lipid (IHCL) can lead to one of the most common forms of chronic liver diseases in adults, the non-alcoholic fatty liver disease (NAFLD), that comprises a range of liver disorders including hepatic steatosis, an advanced stage of which can result in liver cirrhosis. Many diseases are known to be associated with the retention of IHCL including, but not limited to, obesity and type II diabetes. It is, thus, essential to quantify IHCL for early diagnosis and monitoring for an effective treatment. This study presents a noninvasive, robust and reproducible approach to quantify IHCL content using the single-voxel 1H magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) at 3 Tesla (3T). The study is divided in two parts: the MRS and the MRI investigations. Both parts used the constructed peanut oil phantoms of known fat fractions (8%, 14%, 18%, 25%, 30%, 40%, 45%, 55%) to test for validation and accuracy. The results with ...
Scientific reports, 2018
Non-alcoholic fatty liver disease (NAFLD) is recognized as a liver manifestation of metabolic syndrome, accompanied with excessive fat accumulation in the liver and other vital organs. Ectopic fat accumulation was previously associated with negative effects at the systemic and local level in the human body. Thus, we aimed to identify and assess the predictive capability of novel potential metabolic biomarkers for ectopic fat depots in non-diabetic men with NAFLD, using the inflammation-associated proteome, lipidome and metabolome. Myocardial and hepatic triglycerides were measured with magnetic spectroscopy while function of left ventricle, pericardial and epicardial fat, subcutaneous and visceral adipose tissue were measured with magnetic resonance imaging. Measured ectopic fat depots were profiled and predicted using a Random Forest algorithm, and by estimating the Area Under the Receiver Operating Characteristic curves. We have identified distinct metabolic signatures of fat depo...