A Study on the Relationship of Hepatitis B Virus with Hepatocellular Carcinoma (original) (raw)

2014, IOSR Journal of Pharmacy and Biological Sciences

Hepatitis B virus (HBV) has infected approximately 2 billion peo ple worldwide of which more than 350 million are chronically infected life threatening liver disease. This is a study on the relationship of hepatitis B surface antigen (HBsAg) with hepatocellular carcinoma in patients visiting National hospital, Abuja, State house clinic, Abuja and Federal Medical Centre, Keffi, was carried out in 50 liver tissue samples using histochemical reaction of orcein shikata and Haematoxylin and eosin (H and B) staining techniques. Of the 50 samples collected 20 were from National hospital, Abuja, 10 from State house clinic and 20 were from Federal medical centre, Keffi. Sixteen (32%) were diagnosed hepatocellular carcinoma, 10 (20%) liver cirrhosis, 10 (20%) liver hyperplasia and 14 (28%) chronic hepatitis. Out of the 16 hepatocellular carcinoma, 12 (75%) were positive for HBsAg (25%) were negative. For 10liver cirrhosis 7 (30%) were negative. The rest were 8 (80%) positive for hyperplasia out of the 10 samples and all the 14 (100%) cases of chronic hepatitis were positive for HBsAg. Generally, of the 50 samples investigated, 41 (82%) were positive for HBsAg while 9 (18%) were negative. This shows a positive relationship on the pattern of HBsAg amongst the liver diseases under study, and of the two staining techniques employed, orcein shikata gave a better result with ease in recognition and quick screening.