Effect of Nigella sativa on Glucose Concentration, Lipid Peroxidation, Anti-Oxidant Defence System and Liver Damage in Experimentally-Induced Diabetic Rabbits (original) (raw)
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HEPATOPROTECTIVE EFFECT OF NIGELLA SATIVA ON DIABETIC MICE
Diabetes mellitus is a major health problem for the people of the world. Diabetes is a chronic metabolic disorder resulting from a variable interaction of hereditary and environmental factors and it is characterized by abnormal insulin secretion or insulin receptor affecting ? cells of pancreas. Itis associated with a number of chronic complications including nephropathy, neuropathy, retinopathy and cardiovascular diseases. Diabetes mellitus affects a large number of people throughout the world and India also. Experts estimate that diabetic population will grow from 195 to 360 million by 2030 almost 4.5 percentage of the global population. Present study included histological and biochemical parameters of mice. Three groups of mice were prepared for comparative study on control, diabetic, and N. sativa .Diabetic models were prepared in mice by intraperitoneal administration of single dose of alloxan@120mg/kg b.w. Alcoholic extract of Nigella sativa was administered @100 mg/kg b.w/day for four and eight weeks. In diabetic group of mice glucose, creatinine, urea and SGPT were increased. Effective restoration was observed in glucose, SGPT, urea and createnine of N. sativa administered diabetic group of mice. Liver also shows effective restoration in N. sativa administered group of mice. Thus, it is concluded from study that alcoholic extract of N.sativa restores glucose level to normal. Nigella sativa acts effectively on diabetes mice on biochemical and histological parameters.
Biochemical effects of Nigella sativa L seeds in diabetic rats
Indian journal of …, 2006
Oral administration of ethanol extract of N. sativa seeds (300 mg/kg body weight/day) to streptozotocin induced diabetic rats for 30 days significantly reduced the elevated levels of blood glucose, lipids, plasma insulin and improved altered levels of lipid peroxidation products (TBARS and hydroperoxides) and antioxidant enzymes like catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase in liver and kidney. The results confirm the antidiabetic activity of N. sativa seeds extract and suggest that because of its antioxidant effects its administration may be useful in controlling the diabetic complications in experimental diabetic rats.
International Journal of Integrative Medical Sciences, 2016
Introduction: Malondialdehyde (MDA) cause toxic stress in cells and form covalent protein and its used as a biomarker to measure the level of oxidative stress. There is opposite mechanism which control the flux of reactive oxygen species (ROS) called as antioxidant system, which includes both enzymatic andnon-enzymatic components. The important enzyme of this system is superoxide dismutase (SOD). The black cumin or Nigella Sativaseeds are used in traditional medicine of different countries. Thymoquinone is major bioactive component of nigellasativa seed and it is the cause of therapeutic property of Nigella sativa seed. The present study is conducted to see the effect of Nigella Sativa seed powder and Thymoquinone on Serum MDA and SOD levels in normal and Streptozotocine Induced Diabetic Rats. Materials and Methods: 36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/ Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats, one group served as diabetic rats received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW). Results: There is no change in MDA and SOD levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels of MDA are increased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the MDA levels are decreased significantly(p<0.05). The levels of SOD are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the SOD levels are increased significantly. Conclusion: The results of these study concluding that nigella sativa seed powder and thymoquinone has having antioxidant effect in diabetic rats which lowering the MDA levels and increasing the SOD levels in streptozotocine induced diabetic rats.
Protective Effect of Nigella Sativa Against Diabetic Complications on the Liver in White Male Rats
The Egyptian Journal of Hospital Medicine, 2013
Aim of the work: The effect of diabetes on the liver is associated with histological changes. The aim of the present study was to evaluate the histological changes following administration of nigella sativa (NS) in the streptozotocin (STZ) induced diabetes mellitus in rats. Materials and Methods: Thirty six male white rats (n=36), weighing (180-230 g) were taken for this study. The animals were divided into 3 groups: 1-Normal control group. 2-Diabetic group. 3-Diabetic group treated by Nigella Sativa (N.S) oil. Diabetes was induced in the experimental rats via intraperitoneal injection of streptozotocin (45 mg/kg body weight) in a single dose. The fasting blood glucose was estimated, 5ml\kg of body weight of N. sativa oil was given orally for three weeks. After an overnight fast, the animals were sacrificed. The livers were identified, weighed and observed for any gross appearance and color changes and tissues were preserved for histopathological studies using hematoxylin & eosin (H&E) and periodic acid Schiff (PAS) stains. Results: In streptozotocin treated animals, the body weight was significantly decreased compared to normal rats, while treating diabetic rats with N. sativa oil showed significant increases in the body weight. Administration of nigella sativa oil to diabetic rats resulted in a significant decrease in blood glucose after three weeks compared to untreated diabetic rats. In untreated diabetic group, there was a significant decrease in the liver glycogen. Light microscopic examination of the liver of diabetic rats revealed profound histological changes. Nigella sativa consumption could reverse most of these histological and biochemical changes in the liver of the diabetic group owing to its hypoglycemic and antioxidant effect. Conclusion: The Nigella Sativa due to its antioxidant role may be helpful in reversing the changes in the liver in diabetes mellitus.
Antidiabetic potential of Nigella sativa L seed oil in alloxaninduced diabetic rabbits
Tropical Journal of Pharmaceutical Research
Purpose: To evaluate the antidiabetic, hypolipidemic and antioxidant potential of seed oil of Nigella sativa L (NSO). Methods: Nigella sativa seed oil (NSO) was extracted with Soxhlet apparatus using petroleum ether, and was given orally at a dose of 2.5 ml/kg body weight to alloxan-induced diabetic rabbits daily for 24 days. Biochemical parameters including total cholesterol (TC), triglycerides (TGs), low density lipoprotein cholesterol (LDL), very low lipoprotein cholesterol (VLDL), high density lipoprotein (HDL) and plasma glucose were determined in the treatment and control groups. Furthermore, bilirubin, vitamin C, catalase and mean body weight were assessed. Results: NSO treatment significantly lowered serum blood glucose levels and lipid contents, but increased the mean body weight, HDL-C and vitamin C levels of diabetic rabbits (p < 0.001). Moreover, NSO significantly decreased catalase activity, TC, TGs, LDL-C and VLDL-C levels, but normalized bilirubin levels in diabeti...
International Journal of Anatomy and Research, 2016
Background: Diabetes mellitus is associated with biochemical, physiological and pathological alterations in the liver. Liver damage is associated with the formation of oxygen derived free radicals. Reactive oxygen species as well as nitrogen species are responsible for nuclear DNA fragmentation and cell death. The aim of this study was to investigate the hepatoprotective effects of Nigella sativa seed. Materials and Methods: This work was conducted as part of Ph.D work under Department of Anatomy, Shri BM patil Medical College, BLDE University, Bijapur. University ethical committee and Institution Animal Ethical committee are approved the work according to CPCSEA (Govt of India) Rules. 18 rats were selected for this study and divided in to 3 groups each contains 6 rats, one group served as normal control, one group served as Streptozotocin (50mg/kg BW) induced diabetic control rats and one group served as diabetic rats treated with the nigella sativa seed powder(300mg/Kg body weight). Results: Histopathological observations of present study showed that nigella sativa treatment has proved its hepatoprotective effect in streptozotocin induced diabetic rats. The liver enzyme parameters significant change also supported histopathological findings. Conclusion: The results of present study concluding that Nigella sativa seed powder having hepatoprotective effect in streptozotocine induced diabetic rats.
2021
Metabolic disorder diabetes results from an alteration of the secretion or action of insulin. Nigella sativa is a traditionally used specimen since ancient times. We aimed to investigate the hypoglycemic potential of ethanolic extract of Nigella sativa seed powder solution both in a dose and source-dependent manner as well as to fathom out its safety profile so that this plant can be used to ameliorate diabetes. Diabetes was induced in the rat model via intraperitoneal injection of alloxan (150 mg/kg). Ethanolic extract of T. Nigella sativa was administered to rats’ belonged to different groups. Blood glucose levels were assessed periodically and the safety profiles were evaluated through assessment of SGOT, SGPT, creatinine, and lipid profiles after sacrificing the animals. It has been evidenced that Nigella sativa possesses anti-diabetic activity. Furthermore, the extract is capable of reversing the disturbed pathological state towards a healthy status. Besides, these therapeutic ...
BMC Complementary and Alternative Medicine, 2014
Background: Nigella sativa fixed (NSFO) and essential (NSEO) oils have been used to treat diabetes mellitus and its complications. Present study was undertaken to explore and validate these folkloric uses. Methods: Sprague dawley rats having streptozotocin (STZ) induced diabetes mellitus were used to assess the role of NSFO and NSEO in the management of diabetes complications. Parameters investigated were antioxidant potential, oxidative stress, and the immunity by in vivo experiments. Results: The results indicated that STZ decreased the glutathione contents (25.72%), while NSFO and NSEO increased the trait significantly (P < 0.05). Experimental diets increased the tocopherol contents (P < 0.01) and enhanced the expression of hepatic enzymes (P < 0.01). Correlation matrix further indicated that antioxidant potential is positively associated (P < 0.05) responsible for the modulation of hepatic enzymes and the decrease of the nitric oxide production thus controlling the diabetes complications.
The objective of this study was to evaluate the possible antidiabetic and insulin tropic effect of nigella sativa seeds water extract and/or lipoic acid. For this purpose, Forty male Wistar strain albino rats were divided into five groups (8 rat /group), group 1; control group, group2; normal rats fed on basal diet and received 21% fructose in drinking water for four weeks then injected intraperitoneally by a single streptozotocin (STZ) dose (40 mg/kg) to induce type2 diabetes and served as diabetic control group , group3; diabetic rats orally administrated water extract of nigella sativa (1ml/kg daily for 6 weeks), group4; diabetic rats orally administrated lipoic acid (100 mg/kg daily for 6 weeks), group5;diabetic rats orally administrated aqueous extract of nigella sativa and lipoic acid daily simultaneously at the previous tested doses for 6 weeks. Untreated diabetic group showed a significant increase in serum glucose, total cholesterol, triacylglycerols, transforming growth factor beta (TGF- β)and leptin. Also, hepatic total lipids, total cholesterol, alcohol dehydrogenase (AD) activity were significantly elevated in untreated diabetic group as compared to normal control. Whereas, serum insulin, C-peptide, insulin like growth factor -1(IGF-1), hepatic glycogen and NADPH were significantly reduced in untreated diabetic group as compared to normal control. Moreover, the activities of hepatic enzymes cytochrome p450 reductase, glucose-6-phosphate dehydrogenase (G-6-P-D), Glyoxalase I and II were significantly reduced in untreated diabetic rat as compared to normal control group (p<0.05). Oral administration of nigella sativa seeds water extract and /or lipoic acid restored all these altered biochemical parameters to near normal levels. Conclusion the ameliorative effect of water extract of nigella sativa and /or lipoic acid on altered carbohydrate , lipid and oxidative stress variables in type 2 diabetic rats , may be attributed to their insulin releasing capacity, lipid lowering effect and antioxidative effect.
Plos One, 2015
Background and Objective Oxidative stress plays an important role in pathogenesis of diabetes mellitus and its complications. Our previous study has shown glucose lowering effect produced by 3 months supplementation of Nigella sativa (NS) in combination with oral hypoglycemic drugs among type 2 diabetics. This study explored the long term glucose lowering effect (over one year) of NS in patients with type 2 diabetes mellitus on oral hypoglycemic drugs and to study its effect on redox status of such patients. Methods 114 type 2 diabetic patients on standard oral hypoglycemic drugs were assigned into 2 groups by convenience. The control group (n = 57) received activated charcoal as placebo and NS group (n = 57) received 2g NS, daily, for one year in addition to their standard medications. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA 1c), C-peptide, total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), glutathione and thiobarbituric acid reactive substances (TBARS) at the baseline, and every 3 months thereafter were determined. Insulin resistance and β-cell activity were calculated using HOMA 2 calculator. Results Comparison between the two groups showed a significant drop in FBG (from 180±5.75 to 180±5.