GREEN SYNTHESIS OF SILVER NANOPARTICLES (AgNPs) FROM OCIMUM SANCTUM (TULSI) AND ITS ANTI-MICROBIAL PROPERTIES (original) (raw)
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Shanlax International Journal of Arts, Science and Humanities, 2020
Nanoparticles (NPs) are being widely used in different fields; therefore, there is growing interest in the development of a biological and environmental safety method for their production. Now a day’s chemical and physical methods are being used for the development of nanoparticle that is costly, time-consuming, and harmful for the environment. Plant-mediated synthesis of nanoparticles is a “Green chemistry” approach that connects different types of plants with nanotechnology. It has gained much more attention as a reliable, sustainable, and eco-friendly method for synthesizing a wide range of materials/nanomaterials. Plants are called nature’s “chemical factories” therefore, plants and plant extracts are the best options to produce different types of nanoparticles. In this present study, silver nanoparticles were synthesized by using leaf extract of Ocimum sanctum and aqueous silver nitrate solution through a simple and eco-friendly method. Then the developed silver nanoparticles w...
Journal of Nanoparticle …, 2011
Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV-vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV-vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and grampositive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4-30 nm possessing antimicrobial activity suggesting their possible application in medical industry.
2019
Ocimum gratissimum leaves were used in the synthesis of silver nanoparticles (AgNps) that were non-toxic and also possessed antimicrobial properties against clinically isolated pathogenic strains. Some bioactive agents in the aqueous extract of Ocimum gratissimum leaves (AEOgL) were identified. AgNp synthesis was carried out by incubating the AEOgL and 1 mM AgNO3. The brownish colour obtained upon reduction of silver by the AEOgL was observed. The phytochemicals present are tannins, glycosides, sterols, phenols, alkaloid and terpenoids. There was the presence of a surface plasmon resonance from the UV-visible scan. The synthesized silver nanoparticles were characterized by UV-visible spectrophotometry, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy/Energy Dispersive Analysis (SEM/EDX) and X-ray diffraction analysis. The SEM/EDX analysis indicated that the morphology of the nanoparticles is of a uniform structure and the size of the AgNp was 20 nm. The A...
Organic and medicinal chemistry letters, 2014
Background: Nanotechnology is now regarded as a distinct field of research in modern science and technology with multifaceted areas including biomedical applications. Among the various approaches currently available for the generation of metallic nanoparticles, biogenic synthesis is of increasing demand for the purpose of green nanotechnology. Among various natural sources, plant materials are the most readily available template-directing matrix offering cost-effectiveness, eco-friendliness, and easy handling. Moreover, the inherent pharmacological potentials of these medicinal plant extracts offer added biomedical implementations of the synthesized metal nanoparticles. Results: A robust practical method for eco-friendly synthesis of silver nanoparticles using aqueous leaf extract of Ocimum sanctum (Tulsi) as both reducing and capping agent, under the influence of direct sunlight has been developed without applying any other chemical additives. The nanoparticles were characterized with the help of UV-visible spectrophotometer and transmission electron microscopy (TEM). The prepared silver nanoparticles exhibited considerable antibacterial activity. The effects were more pronounced on non-endospore-forming Gram-positive bacteria viz., Staphylococcus aureus, Staphylococcus epidermidis, and Listeria monocytogenes than endospore-forming species Bacillus subtilis. The nanoparticles also showed prominent activity on Gram-negative human pathogenic Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, and plant pathogenic Pantoea ananatis. A bactericidal mode of action was observed for both Gram-positive and Gram-negative bacteria by the nanoparticles.
Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property
Journal of Saudi Chemical Society, 2012
Plants extract from Ocimum tenuiflorum, Solanum tricobatum, Syzygium cumini, Centella asiatica and Citrus sinensis was used for the synthesis of silver nanoparticles (Ag NPs) from silver nitrate solution. Ag NPs were characterized by UV-vis spectrophotometer, X-ray diffractometer (XRD), atomic force microscope (AFM) and scanning electron microscope (SEM). The formation and stability of the reduced silver nanoparticles in the colloidal solution were monitored by UV-vis spectrophotometer analysis. The mean particle diameter of silver nanoparticles was calculated from the XRD pattern according to the line width of the plane, refraction peak using the Scherrer's equation. AFM showed the formation of silver nanoparticle with an average size of 28 nm, 26.5 nm, 65 nm, 22.3 nm and 28.4 nm corresponding to O. tenuiflorum, S. cumini, C. sinensis, S. tricobatum and C. asiatica, respectively. SEM determination of the brown color stable samples showed the formation of silver nanoparticles and well dispersed nanoparticles could be seen in the samples treated with silver nitrate. Antimicrobial activity of the silver bio-nanoparticles was performed by well diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. The highest antimicrobial activity of silver nanoparticles synthesized by S. tricobatum, O. tenuiflorum extracts was found against S. aureus (30 mm) and E. coli (30 mm) respectively. The Ag NPs synthesized in this process has the efficient antimicrobial activity against pathogenic bacteria. Of these, silver nanoparticles are playing a major role in the field of nanotechnology and nanomedicine.
2020
The silver nanoparticles have found prominence in different fields such as medicine, catalysis, nanoelectronics, textile field, pollution and water treatment due to their unique attributes. Applications of silver nanoparticles are increasing rapidly in the medical purpose including drug delivery, treatment, diagnosis, medical device coating. Various chemical and physical methods are use to synthesize the silver nanoparticles (AgNP) conventionally. But these synthesis processes are expensive and also involves side effects. To solve these savior problems by modification in synthesis process for safer and more efficiency, Green Nanotechnology comes to play a very crucial role for synthesis of
Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract
Current Science, 2019
In the present study, green synthesis of stable silver nanoparticles was done using methanolic leaf extract of Azadirachta indica L. as well as Ocimum sanctum L. The antimicrobial activity was screened against UTI causing bacteria, viz. Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using disc diffusion method. Silver nanoparticles derived from tulsi leaf extract inhibited E. coli by 12.00 ± 0.65 mm, K. pneumoniae by 9.66 ± 0.11 mm and S. aureus by 23.00 ± 0.81 mm at concentrations 1 mg/ml. Similarly, silver nanoparticles derived from neem leaf extract inhibited K. pneumoniae by 9.11 ± 0.81 mm and S. aureus by 09.0 ± 1.17 mm at concentrations 1 mg/ml respectively. Lower concentration (1 mg/ml) of tulsi and higher concentration (4 mg/ml) of neem leaf methanolic extract was observed to be effective against urinary tract infections in human causative agent S. aureus.
PeerJ
The biosynthesis of nanoparticles using the green route is an effective strategy in nanotechnology that provides a cost-effective and environmentally friendly alternative to physical and chemical methods. This study aims to prepare an aqueous extract of Ocimum sanctum (O. sanctum)-based silver nanoparticles (AgNPs) through the green route and test their antibacterial activity. The biosynthesized silver nanoparticles were characterised by colour change, UV spectrometric analysis, FTIR, and particle shape and size morphology by SEM and TEM images. The nanoparticles are almost spherical to oval or rod-shaped with smooth surfaces and have a mean particle size in the range of 55 nm with a zeta potential of −2.7 mV. The antibacterial activities of AgNPs evaluated against clinically isolated multidrug-resistant Acinetobacter baumannii (A. baumannii) showed that the AgNPs from O. sanctum are effective in inhibiting A. baumannii growth with a zone of inhibition of 15 mm in the agar well diff...
2019
To resolve the efficacies of antibacterial, antifungal, and antioxidant activities of aqueous leaf extract of Ocimum basilicum mediated biosynthesis of silver nanoparticles (Ag-NPs). Aqueous and synthesized Ag-NPs were tested by disc diffusion method against the bacterial and fungal pathogens. Antioxidant activity of synthesized Ag-NPs and aqueous plant extract were determined. The scavenging radicals were estimated by DPPH method. The synthesized Ag-NPs were characterized by XRD, FT-IR, FESEM and EDX. XRD peaks at 2θ values of 27.6°, 32.2°, 38.16°, and 46.04° representing the (110), (111), (121), and (200). FT-IR absorption peak located at 2910 cm-1 are due to C–H stretch of alkenes and a peak at 1669 cm-1 due to -C=C- band of alkenes and a peak at 1614 cm-1 is assigned to N-H bend of 1° amines and a peak at 1092 cm-1 due to C–N stretch of aliphatic amines and a peak at 875 cm-1 are due to C–H stretch of aromatics. TEM study reveals that most of the nanocrystals formed were spheric...