Toxicity Test on Anogeissus leiocarpus Plant with Potential for use as Tea Substitute (original) (raw)

2016, International Journal Of Scientific Research And Education

Powdered leaves of Anogeissus leiocarpus were extracted with ethanol and fractionated with Pet-ether, Nhexane, Ethyl acetate, Chloroform, and Ethanol using partition technique. The extracts and fractions were tested for toxicity on brine shrimp larvae and phytochemically screened for the presence of secondary metabolites. Brine shrimp test result showed that the fraction of ethyl acetate was found to be more toxic with LC 50 of 0.0017mg/ml. The fraction obtained from chloroform was found to be moderately toxic with LC 50 of 18.58 mg/ml to brine shrimps. Concentrations of 10, 100, 1000 in mg/ml of extracts were administered. The lethal concentrations (LC 50) at lower concentrations (10mg/ml) were less toxic and are highly toxic at higher concentrations. The Phytochemical screening of the fractions revealed the presence of Alkaloids, Flavonoids, Steroids, Reducing sugars, and Tannins. Thus it is appropriate to use the plant as a tea substitute.