Binding of Acf1 to DNA Involves a WAC Motif and Is Important for ACF-Mediated Chromatin Assembly (original) (raw)

This study investigates the binding of Acf1 to DNA and its role in ACF-mediated chromatin assembly. The WAC motif was identified as crucial for the interaction of Acf1 with DNA, with experimental results demonstrating that mutations in this region impair the ATPase activity of the ACF complex and its ability to assemble chromatin effectively. Findings underscore the importance of precise molecular interactions in chromatin dynamics and provide insights into the mechanistic roles of ACF in these processes.