Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-Producing Escherichia coli among Uropathogens of Pediatrics in North of Iran (original) (raw)
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British Journal of Medical and Health Sciences, 2012
Encreased prevalence of Escherichia coli resistant to extended spectrum beta-lactamase (ESBL) has been reported all over the world and is rapidly changing over time, especially in Asia-Pacific region. The aim of this study was to determine the frequency of ESBL genes (blaTEM, blaSHV and blaCTX-M) among E. coli isolates causing urinary tract infections in an Iranian referral pediatrics center. In this study, 184 hospital and community isolates of E. coli cultured from urine samples between July 2009 and December 2010 were screened for ESBL production. ESBL production was detected using a double-disc synergy test and presence of blaCTX-M, blaSHV and blaTEM beta -lactamase genes was evaluated by PCR. Among the E. coli strains isolated of each group, 22 isolates (24.4%) of group1, 3 isolates (17.6%) of group 2 and 34 isolates (44.1%) of group 3 were found to harbor ESBL producers. Twenty of 59 ESBL positive isolates carried more than one type of beta-lactamase genes. Interestingly, 3 is...
Journal of Medical Microbiology and Infectious Diseases, 2014
Introduction: Extended spectrum beta-lactamase (ESBL) producing Escherichia coli generate a major problem for clinical therapeutics and epidemiological study. The incidence of ESBL producing strains among clinical isolates has been steadily increasing during the past few years, and remains an important cause of failure of therapy with cephalosporins. The aim of this study was to determine the antimicrobial susceptibility pattern and prevalence of ESBLs in E. coli isolates taken from different clinical specimens by phenotypic and genotypic techniques. Methods: In this descriptive study, a total of 100 E. coli isolates collected from different clinical specimens were used. The antibiotic resistance pattern to twelve antimicrobial agents was determined by disk diffusion method. The ESBLs producing strains were confirmed by double-disk-diffusion test, and the CTX-M, TEM, SHV, and OXA were detected by PCR. Results: The prevalence of ESBL producing E. coli was 56%. The results show that 95% of ESBL producing E. coli isolates tested were resistant to ceftriaxone and cefotaxime, 93% for ceftazidime, 86% for azithromycin, 79% for cefazolin and 43% to imipenem. Among the ESBL producing E. coli, 48%, 30% and 11% were positive for CTX-M, TEM and SHV genes, respectively. OXA was not found in all isolates. Conclusion: ESBL producing isolates of E. coli have been increasingly recognized and there is a need to carefully formulate therapeutic strategies to control infections in teaching Hospitals. The high percentage of drug resistance in ESBL producing E. coli suggests that routine detection of ESBL is required by reliable laboratory methods.
Infection and Drug Resistance, 2018
Introduction: Enteroaggregative Escherichia coli (EAEC) has been implicated as an emerging cause of traveler's diarrhea, persistent diarrhea among children, and immunocompromised patients. The present study aimed to investigate the prevalence of antibiotic resistance, extendedspectrum β-lactamase (ESBL) production, and virulence factors of EAEC isolates obtained from Iranian children suffered from diarrhea. Materials and methods: In this cross-sectional study, from March 2015 to February 2016, 32 EAEC isolates were collected from fecal samples of children aged <12 years with diarrhea in southwest of Iran. All EAEC isolates identified using phenotypic and molecular methods and the cell line adhesion assay. Antimicrobial susceptibility testing was determined using disk diffusion method. The presence of virulence factors and ESBL resistance genes were determined by polymerase chain reaction. Results: Overall, 28.1% (9/32) of the isolates were positive for at least one of virulence genes. The most frequent gene was aap with a frequency of 96.9%. Neither aafA nor aggA gene was detected among all of the EAEC isolates. Antimicrobial susceptibility testing revealed the highest resistance rate to ampicillin (100%) and co-trimoxazole (100%), followed by ceftriaxone (81.3%). Further analysis revealed that the rate of ESBLs-producing isolates was 71.9% (23/32). Polymerase chain reaction screening revealed that 87.5% and 65.5% of EAEC isolates were positive for bla TEM and bla CTX-M genes, respectively, and 17 (53.1%) of isolates contained both bla TEM and bla CTX-M genes. Conclusion: The high detection rate of ESBL-producing EAEC isolates accompanied with virulence genes highlights a need to restrict infection control policies in order to prevent further dissemination of the resistant and virulent EAEC strains.
Avicenna Journal of Clinical Microbiology and Infection, 2015
Background: Beta-lactam antibiotics such as long-spectrum cephalosporins and carbapenems are the empirical treatment against urinary tract infections (UTIs). The common mechanism of resistance to B-lactamas in Escherichia coli is producing of ESBLs. Objectives: The aims of this study was evaluation of the susceptibility of the ESBL-producing E. coli strains to ordinarily used antibiotics and to detect the presence of the four common ESBL genes: blaTEM, blaOXA, blaCTX-M and blaSHV using multiplex PCR method. Materials and Methods: In a retrospective cross sectional study conducted between 2012 and 2013, out of145 strains of E. coli were isolated from hospitalized children with UTIs. Susceptibility test was performed by disc diffusion method. All isolates were also tested for ESBL by the standard CLSI double disk diffusion method, using cefotaxime/ clavulanic acid and ceftazidime/clavulanic acid disks. Multiplex PCR was performed for detect of blaCTX-MIV, blaOXA, blaTEM and blaSHV genes. Results: From 145 strains of E. coli, 51(35.2%) and 61(42.1%) isolates were resistance to ceftazidime and cefotaxime, respectively. ESBL production was noted in 59 (40.7%) strains. The amplification of β-lactamase genes showed the presence of blaCTX-M like genes in 22 (37.9%) strains, blaTEM in 42 (72.4%), blaSHV in 7 (11.9%) and blaOXA in 23 (39%) of the total 58 strains of E. coli. Conclusions: Our data showed that, the high prevalence of beta-lactamase genes among isolates, and it. Our findings may provide useful insights in replace of the appropriate antibiotics and it may also prevents of ESBLs, mediated resistance problem.
Introduction: One of the most important resistance determinants in Enterobacteriaceae are extended spectrum β-lactamases (ESBLs). During the last decade, CTX-M types ESBLs have increased considerably and become the most common ESBLs worldwide which are the major causes of the urinary tract infections (UTIs). The aim of this study was to determine the antibiotic resistance patterns and the frequency of the CTX-M β-lactamases among multi-drug resistant (MDR) Escherichia coli (E. coli) isolates from northern Iranian patients with UTI. Materials and methods: Thirty three E. coli isolates from urine samples were applied in this study. Double disk synergy test (DDST) was applied for identification of ESBL phenotypes in E. coli isolates. The ESBL related genes, CTX-M group (1, 2, 8 and 9), were amplified by polymerase chain reaction (PCR). Results: All E. coli isolates showed sensitivity to piperacillin and 55% of the isolates were resistant to 3rd and 4th cephalosporins. The presence of the blaCTX-M gene in 88% of the ESBL producing isolates was approved based on molecular method. CTX-M (1, 2, 8 and 9) containing E. coli isolates showed resistance to more antibiotics than non-CTX-M isolates. CTX-M-1 was the most prevalent CTX-M determinant in ESBL producing E. coli isolates. Discussion and conclusion: Based on the results of the present study, the preferred antibiotic against CTX-M type ESBL-producing E. coli strains in north of Iran, Rasht, should be piperacillin. Although, CTX-M type ESBLs prevalence was nearly low in the studied MDR E. coli isolates, but controlling these low prevalence isolates is important. Key words: CTX-M, E. coli, ESBL, Multi-drug resistance, Urinary tract infection
Annali di igiene : medicina preventiva e di comunita, 2019
BACKGROUND Urinary tract infections (UTIs) are a highly prevalent infection among children and Escherichia coli is one of the most important pathogens causing pediatric UTIs. Production of extended spectrum b-lactamase (ESBL) enzymes is an important factor in the emergence of antibiotic resistance among these bacteria. This study aimed to determine the resistance patterns, the frequency of ESBL-encoding genes and the genetic diversity of E. coli strains isolated from children with UTIs who were admitted to children's referral hospital of Hazrat Masoumeh, Qom, Iran. METHODS A total of 102 consecutive non-duplicative strains of E.coli that were isolated from children with UTIs were included into the study. Antibiotic susceptibility of the isolates was determined by disk diffusion method according to the CLSI guidelines. The ability of the isolates to produce ESBLs was phenotypically determined by both combined disk test and double disk synergy test. The ESBL encoding genes (bla CT...
Background: Extended spectrum β-lactamase (ESBL) producing Escherichia coli has tremendously increased worldwide and it is one of the most common causes of morbidity and mortality associated with hospital-acquired infections. This could be attributed to association of multi drug resistance in ESBL producing isolates. The present study was aimed to determine the antimicrobial resistance profile of ESBL producing Escherichia coli isolates from various clinical samples. Methods: In this study,204 cases of Escherichia coli within 7 months from patients referring to the public and private treatment centers Tabriz city were collected and identified by biochemical tests. ESBL screening and confirmation along with antimicrobial resistance test was done according to the Clinical Laboratory Standards Institute (CLSI) guidelines. Results: Of the 204 isolates, 110 (53.92%) were identified as having ESBL phenotype. Over 90.2% of ESBL isolates showed resistance to ampicillin . 83.6% of ESBL isolates were imipenem Sensitive. Conclusion: The prevalence of ESBL is increasing day by day in nearly every center of different countries and necessary steps to prevent the spread and emergence of resistance should be taken.
Zagazig university medical journal, 2013
To determine the prevalence and the antibiotic resistant patterns of the multi-drug resistant Extended-Spectrum Β-Lactamase(ESBL) producing E. coli isolates from children urine samples, in Queen Rania Al-Abdullah Hospital for children.A total of 61 non-repetitive urine samples from various outpatient clinics and inpatient wards were collected retrospectively over a period of 5 months (May 2012 to September 2012). The resistant patterns, screening and confirmatory tests for phenotypic detection of ESBL-producers were studied using the VITEK 2 system against a set of antibiotics found on the antimicrobial susceptibility extend card AST-EXN8.Children were nearly equally infected by both types of E. coli isolates, ESBL-producers 31 (50.8%) and non ESBL-producers 30 (49.2%). ESBL-producing E. coli showed maximum rate resistance to Cefuroxime and Piperacillin (100%), Aztreonam, Cefixime, Ceftriaxone plus Levofloxacin (96.8%), Ampicillin/Sulbactam and Cefepime (93.5%), and Moxifloxacin (90...