Antibacterial Activity of In Situ Prepared Chitosan/Silver Nanoparticles Solution Against Methicillin-Resistant Strains of Staphylococcus aureus (original) (raw)
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Vietnam Journal of Science and Technology, 2018
A green and simple approach has been successfully developed to synthesize chitosan/Ag nanocomposites using kumquat extract as a biological reducing agent. It indicates to be an eco-friendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size ~15-25 nm. Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against S. aureus and E. coli. The chitosan/Ag nanocomposite was found to have significantly higher antimicrobial activity than its components at their respective concentrations. The presence of a small percentage (2.5 %, w/w) of metal nanoparticles in the nanocomposite was enough to significantly enhance inactivation of S. aureus and E. coli as compared with unaltered ch...
2018
A simple and eco-friendly approach has been successfully developed for the preparation of chitosan nanocomposites films loaded with silver nanoparticles using kumquat extract as a biological reducing agent. The chitosan/silver nanocomposites (CTS/Ag NCPs) films were prepared from chitosan/silver nanocomposites solution and dried for 14 h at 70C in a vacuum oven with the pressure of 0.03 Mpa. The morphology and characterization of CTS/Ag NCPs films have been also determined by FTIR, XRD, and SEM. The UV-vis spectroscopy and TEM image indicated that synthesized chitosan/silver nanocomposites have spherical shape with their uniform dispersion and their average particle size of about 20-30 nm. The prepared CTS/Ag NCPs films showed their great antibacterial activity on Staphylococcus aureus (S. Aureus) and Escherichia coli (E. coli). Therefore, this eco-friendly method that would be used for the preparation of chitosan/Ag nanocomposites films could be competitive and alternative to the e...
Synergistic antibacterial activity of chitosan–silver nanocomposites on Staphylococcus aureus
Nanotechnology, 2011
The approach of combining different mechanisms of antibacterial action by designing hybrid nanomaterials provides a new paradigm in the fight against resistant bacteria. Here, we present a new method for the synthesis of silver nanoparticles enveloped in the biopolymer chitosan. The method aims at the production of bionanocomposites with enhanced antibacterial properties. We find that chitosan and silver nanoparticles act synergistically against two strains of Gram-positive Staphylococcus aureus (S. aureus). As a result the bionanocomposites exhibit higher antibacterial activity than any component acting alone. The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of the chitosan-silver nanoparticles synthesized at 0 • C were found to be lower than those reported for other types of silver nanoparticles. Atomic force microscopy (AFM) revealed dramatic changes in morphology of S. aureus cells due to disruption of bacterial cell wall integrity after incubation with chitosan-silver nanoparticles. Finally, we demonstrate that silver nanoparticles can be used not only as antibacterial agents but also as excellent plasmonic substrates to identify bacteria and monitor the induced biochemical changes in the bacterial cell wall via surface enhanced Raman scattering (SERS) spectroscopy.
Synthesis of Chitosan-silver nanocomposites and their antibacterial activity
International Journal of Scientific and Engineering Research
The present study explores the in situ fabrication of chitosan-silver nanocomposites in view of their increasing applications as antimicrobial packaging, wound dressing and antibacterial materials. Chitosan/Silver nanocomposites were prepared by embedding of silver nanoparticles in chitosan polymer. Synthesis of nanocomposites was confirmed by Fourier Transform Infrared (FTIR) spectroscopy, X-Ray Diffraction (XRD) analysis and Differential Scanning Calorimetry (DSC) etc. In addition, the formed nanocomposites have an average particle size of ~10-15 nm as observed by Transmission Electron Microscopy (TEM). Their antibacterial activity was assessed by zone of inhibition method against Staphylococcus aureus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella entrica MTCC 1253 in vitro.
Advances in Materials Science and Engineering, 2013
The aim of this study is to investigate the antibacterial properties and characterization of chitosan-silver nanoparticle composite materials. Chitosan-silver nanoparticle composite material was synthesized by adding AgNO3and NaOH solutions to chitosan solution at 95°C. Different concentrations (0,02 M, 0,04 M, and 0,06 M) of AgNO3were used for synthesis. Chitosan-silver nanoparticle composite materials were characterized by Transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet (UV) spectrophotometer, and Fourier transform infrared (FTIR) spectrometer techniques.Escherichia coli,Acinetobacter baumannii,Staphylococcus aureus,Enterococcus faecalis,Pseudomonas aeruginosa, andStreptococcus pneumoniaewere used to test the bactericidal efficiency of synthesized chitosan-Ag nanoparticle composite materials. The biological activity was determined by the minimum bacterial concentration (MBC) of the materials. Antibacterial effect of chitosan-silver nanoparticle materia...
Factors Affecting the Antibacterial Activity of Chitosan-Silver Nanocomposite
IET Nanobiotechnology, 2017
This study provides the optimum preparation parameters of chitosan-silver nanoparticles composite (CSNC) with promising antibacterial activity against the most common bacterial infections found on burn wounds. CSNC was synthesised by simple green chemical reduction method with different preparation factors. Chitosan was used to reduce silver nitrate and stabilise silver nanoparticles in the medium. For this reason, spectroscopic and microscopic techniques as, ultraviolet-visible Fourier transform infrared spectroscopy and transmission electron microscopy were used in the study of the molecular and morphological properties of the resultant composites. Furthermore, the composite was assessed in terms of Ag-ions release by AAS and its efficacy as antibacterial material. As a result, CSNC showed stronger antibacterial effect than its individual components (chitosan and silver nitrate solutions) towards Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacteria. CSNC prepared in this study showed highest inhibition percentage of bacterial growth up to 96% at concentration of 220 μg/ml.
Ray assisted synthesis of silver nanoparticles in chitosan solution and the antibacterial properties
Chemical Engineering Journal, 2009
In the present study, chitosan had been utilized as a "green" stabilizing agent for the synthesis of spherical silver nanoparticles in the range of 5-30 nm depending on the percentage of chitosan used (0.1, 0.5, 1.0 and 2.0 wt%) under ␥-irradiation. X-ray diffractometer identified the nanoparticles as pure silver having face-centered cubic phase. Ultraviolet-visible spectra exhibited the influence of ␥-irradiation total absorbed dose and chitosan concentration on the yield of silver nanoparticles. The antibacterial properties of the silver nanoparticles were tested against Methicillin-resistant Staphylococcus aureus (MRSA) (grampositive) and Aeromonas hydrophila (gram-negative) bacteria. This work provides a simple and "green" method for the synthesis of highly stable silver nanoparticles in aqueous solution with good antibacterial property.
Journal of Chemistry
Schiff-bases of chitosan (CS) and carboxymethyl chitosan (CMCS)/silver nanoparticles (AgNPs) have been synthesized, characterized, and evaluated as antimicrobial agents against two Gram +ve bacteria (Bacillus cereus and Staphylococcus aureus) and two Gram −ve bacteria (Escherichia coli and Pseudomonas aeruginosa) in addition to Candida albicans as a fungus. The in situ reactions of CS and/or CMCS with some pyrazole aldehyde derivatives in acidic media containing silver nitrate to yield silver nanoparticles loaded onto CS and CMCS/Schiff-bases were carried out. Characterizations of the prepared compounds via FTIR spectroscopy, SEM, TEM, and TGA were carried out. Schiff-bases/silver nanoparticles of CS and CMCS showed higher antimicrobial activity than the blank CS and CMCS. The presence of AgNO3 (3% wt%) displayed high antibacterial efficiencies with inhibition zones in the extent of 19–39 mm. TEM analysis showed that the size of the silver nanoparticles is in the range of 4–28 nm fo...
Synthesis of Chitosan-Silver Nanoparticle Composite Spheres and Their Antimicrobial Activities
Polymers, 2021
Synthesis of silver nanoparticles–chitosan composite particles sphere (AgNPs-chi-spheres) has been completed and its characterization was fulfilled by UV–vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and zetasizer nano. UV–vis spectroscopy characterization showed that AgNPs-chi-spheres gave optimum absorption at a wavelength of 410 nm. The XRD spectra showed that the structure of AgNPs-chi-spheres were crystalline and spherical. Characterization by SEM showed that AgNPs-chi-spheres, with the addition of 20% of NaOH, resulted in the lowest average particle sizes of 46.91 nm. EDX analysis also showed that AgNPs-chi-spheres, with the addition of a 20% NaOH concentration, produced particles with regular spheres, a smooth and relatively nonporous structure. The analysis using zetasizer nano showed that the zeta potential value and the polydispersity index value of the AgNPs-chi-sphere tended to increase wit...
Comparative Antimicrobial Analysis of Chitosan Nanoparticles With Gentamicin And Chloramphenicol
2019
Chitosan has attained increasing commercial interest as suitable resource materials due to their excellent properties including biocompatibility, biodegradability, adsorption and ability to form films and to chelate metal ions. Antibiotics resistance has become rampant and nanoparticles are increasingly used to target microorganisms as an alternative to antibiotics. The research was conducted to produce and synthesize nanoparticles using the extract of chitosan and test the antimicrobial activity against some selected bacteria. Chitosan was produced by demineralization, deproteinization and deacetylation of oyster shell. The silver nanoparticle was synthesized from Chitosan and screened for in vitro antimicrobial activity against selected bacteria using agar well diffusion method. Synthesized nanoparticles were characterized using UV-VIS spectrophotometry, FTIR spectroscopy and Scanning Electron Microscopy. Chitosan nanoparticles showed some inhibiting properties against the growth of most of the organisms tested (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi). Most of the isolates were inhibited by both extracts. The positive control which was chloramphenicol and gentamycin inhibited the growth of all organisms except Salmonella typhi.