Digital microfluidic platform for high-throughput monoclonal antibodies screening and isotyping analysis (original) (raw)

Monoclonal antibody (mAb) technology is one of the most important scientific achievements in the twentieth century. However, the traditional mAbs screening encounters a bottleneck for the high-throughput process. Here we integrate the immunoassay methods on the digital microfluidic platform for the mAbs analysis that potentially holds high throughput. In our prototype devices, the nitrocellulose dots spotted on the Teflon surface let the isotyping antibodies be bound on the chip stably. Furthermore, the droplet of antibodies mixture and washing buffer were manipulated by EWOD (electrowetting-on-cielectric) force. The preliminary results show that the isotyping analysis was executed on the digital microfluidic platform.