Antifungal activity of the aqueous and hydro-alcoholic extracts of Terminalia superba Engl. Et Diels (Combretaceae) on the in vitro growth of clinical isolates of pathogenic fungi (original) (raw)
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A bioautography method was developed to determine the number of antifungal compounds in Terminalia species extracts. Acetone, hexane, dichloromethane and methanol leaf extracts of six Terminalia species (T. prunioides, T. brachystemma, T. sericea, T. gazensis, T. mollis and T. sambesiaca) were tested against five fungal animal pathogens (Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus, Microsporum canis and Sporothrix schenkii). The R f values and relative activities of separated compounds were determined. Hexane and dichloromethane extracts had at least three times more antifungal compounds than the other extracts indicating the nonpolar character of the antifungal compounds. From the R f values, the non-polar character of the antifungal compounds was confirmed indicating that the antifungal activity is not due to tannins. M. canis had the highest number, up to ten, of antifungal compounds. All Terminalia species contained a compound (R f = 0.46 in benzene/ethanol/ammonium hydroxide (90/10/1) active against all tested pathogens. T. sericea and T. brachystemma were the most promising candidates for isolating antifungal compounds. The results demonstrate the value of bioautography in examining plant extracts with antifungal activity, selecting species for further study and dereplicating the isolation of compounds.
Antifungal activity of Terminalia superba (combretaceae)
Journal of Experimental Biology and Agricultural Sciences, 2015
The aim of the present study was to optimize the anticandidosic activities of Terminalia superba (TEKAM4) and the identification of major compounds present in the most active chromatographic fraction. The hydroethanolic extract TEKAM4-X0 was prepared by homogenization employing a blender. Two derivatives extracts of TEKAM4-X0 (X1-1 and X1-2) were obtained by a liquid/liquid partition of TEKAM4-X0 in a mixture of hexane and water (v/v). Three chromatographic fractions (F1, F2 and F3) from X1-2 were separated by means of Sephadex-LH20 gel filtration chromatography. All the extracts were incorporated to Sabouraud according to the agar slanted double dilution method. Ketoconazole was used as standards for antifungal assay. The entire fractions were tested on the previously prepared medium culture containing 1000 cells of C. albicans. Antifungal activity was determined by evaluating antifungal parameters values (MFC and IC50). Lastly, the structures of 2 isolated compounds were elucidated by combination of Flash chromatography and spectroscopic methods, including MS, and multiple stage RMN experiments. All the article published by Journal of Experimental Biology and Agricultural Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License Based on a work at www.jebas.org.
Journal of Drug Delivery and Therapeutics
Background: Candidiasis is a fungal disease caused by Candida albicans, a yeast that preferentially affects mucous membranes. These diseases are more and more recurrent because of the generalized decline in immunity and the appearance of resistance to classical antifungal drugs. Therefore, fighting against therapeutic failures becomes necessary. The objective of this study is to contribute to the fight against candidiasis through the research of new antifungal molecules. To do so, the hydroalcoholic extracts of Terminalia catappa, Terminalia glaucescens, Terminalia ivorensis, Terminalia mantaly and Terminalia superba, five plant species used in traditional medicine against dermatoses, were tested on the in vitro growth of resistant strains of Candida albicans. In addition, the polyphenol composition of these plants was carried out in order to evaluate their protective powers. Methods: The antifungal tests were performed by the solid-state dilution method. While the determination of ...
Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hydroacoholic extracts from bark of Terminalia ivorensis A. Chev. (Combretaceae). Methods: In vitro antifungal activity of all the extracts was done by agar slant double dilution method. Candida albicans and Aspergillus fumigatus clinically important strains were used for the study. ketoconazole was used as standards for antifungal assay. Antifungal activity was determinated by evaluating of antifungal parameters values which are MCF (minimal concentration fungicide) and IC50 (Concentration for 50% of inhibition) around each assay. Results: Result showed that the antifungal activity was more pronounced against Aspergillus fumigatus than Candida albicans. The hydroalcoholic extract showed best antifungal activity than ketoconazole. Conclusion: Demonstration of antifungal activity of T. ivorensis provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
Journal of Intercultural Ethnopharmacology, 2012
Aim: The present study was undertaken to evaluate in vitro antifungal activity of aqueous and hydroacoholic extracts from bark of Terminalia ivorensis A. Chev. (Combretaceae). Methods: In vitro antifungal activity of all the extracts was done by agar slant double dilution method. Candida albicans and Aspergillus fumigatus clinically important strains were used for the study. ketoconazole was used as standards for antifungal assay. Antifungal activity was determinated by evaluating of antifungal parameters values which are MCF (minimal concentration fungicide) and IC50 (Concentration for 50% of inhibition) around each assay. Results: Result showed that the antifungal activity was more pronounced against Aspergillus fumigatus than Candida albicans. The hydroalcoholic extract showed best antifungal activity than ketoconazole. Conclusion: Demonstration of antifungal activity of T. ivorensis provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antifungal substances that could serve as selective agents for infectious disease chemotherapy.
2021
Parinari curatellifolia (Chrysobalanaceae) and Terminalia sericea (Combretaceae) have been traditionally used in Southern Highlands of Tanzania for treatment of various infectious disorders. The present study aimed to evaluate antifungal activity of the isolated compounds from Parinari curatellifolia and Terminalia sericea plant species. The ethyl acetate extract of the root barks from Parinari curatellifolia and Terminalia sericea were fractionated using column chromatography. The structures of compounds were established using both 1D and 2D-NMR spectroscopic techniques while antifungal activities of the fractions and isolated compounds were evaluated using broth microdilution assay against Candida albicans, Cryptococcus neoformans and Aspergillus niger species. Two known compounds toddalolactone (1) and 10-hydroxy-13-methoxy-9methyl-15-oxo-20-norkaur-16-en-18-oic acid -lactone (2) from P. curatellifolia and two compounds Sericic acid (3) and sericoside (4) from T. sericea were is...
Antimycobacterial, antibacterial and antifungal activities of Terminalia superba (Combretaceae)
South African Journal of Botany, 2010
The methanol extract from the stem bark of Terminalia superba (TSB), fractions (TSB1 -7) and two compounds isolated following bio-assay guided fractionation namely 3,4'di-O-methylellagic acid 3'-O-β-D-xylopyranoside (1) and 4'-O-galloy-3,3'-di-O-methylellagic acid 4-O-β-D-xylopyranoside (2) were evaluated for their antimycobacterial, antibacterial and antifungal activities. The broth microdilution, microplate Alamar Blue assay (MABA) and agar disc diffusion methods were used for the investigations. The results of the antimycobacterial assays showed that the crude extract, fraction TSB5-7 and compound 1 were able to prevent the growth of all the studied mycobacteria. The lowest minimal inhibitory concentration (MIC) value of 39.06 µg/ml for this extract was recorded on both M. smegmatis and M. tuberculosis MTCS2. The corresponding values were 19.53 µg/ml and 4.88 µg/ml for fractions and compounds respectively. The MIC determinations results on other organisms indicate values ranging from 19.53 to 78.12 µg/ml for TSB and compound 2 on 90.9% of the tested organisms, meanwhile compounds 1 as well as fractions TSB 6 and 7 exhibited detectable MIC values on all studied microorganisms. 2
2007
This study has been a long journey that would not have been successfully made if it were for the support of various persons. First and foremost, I would like to acknowledge the support, guidance and encouragement of my supervisor, Prof Jacobus N. Eloff. Without your positive comments and persistent encouragement this work would have simply floundered on and on. I truly want to thank him for listening to my frustrated rantings and bringing me back to a relative level of clarity and calm. For allowing me to disrupt his precious moments at anytime during his busy work. This also goes to, Dr Jackie A. Picard, my co-supervisor, for the constructive criticism, advices, and patience during the course of this research. Her encouragement and keen interest led to satisfactory realization of this study. I would like to extend a sincere word of thanks to Dr Lyndy McGaw who did the proofreading. Thank you for your willingness and the special effort in terms of timing. My sincere thanks also goes to Dr Ladislaus K. Mdee, for helping with structure elucidation and chemical characterization. Special thanks to Dr Joshua Dabrowski (Pathologist), for helping with histopathology studies. My profound gratitude goes to Mr Patrick N. Selahle (Technologist), for helping with rats handling and technical assistance. Ms Denise Marais, for continuous and rapid responses to my administrative questions and queries. Always with a smile. Ms Lita Pauw, for making sure that all necessary equipments and materials are ordered.
Frontiers in microbiology, 2017
Terminalia catappa Linn bark is used to treat dysentery by various populations in Southeast Asian countries, and its leaves have also been used in traditional medicine to treat hepatitis in India and the Philippines. Here, the antifungal actions of crude hydro-alcoholic extract (TcHE) and fractions from T. catappa leaves were assessed via the agar diffusion and microdilution tests on Candida reference strains and clinical isolates from patients with acquired immunodeficiency syndrome (AIDS). Additionally, the potential cytotoxic effects of TcHE were assessed on cultured human peripheral blood mononuclear cells (PBMC). T. catappa fractions and sub-fractions were analyzed by gas chromatography coupled to mass spectrometry with electron impact (GC/MS/EI), high-performance liquid chromatography coupled to mass spectrometry "electrospray" ionization in positive mode (HPLC/MS/MS/ESI(+)) and hydrogen nuclear magnetic resonance ((1)HNMR). TcHE and its fractions were able to inhibi...