Material and Methods (original) (raw)

Abstract

A. caerulea seeds were bought from a gardener shop (Jardiland, Maurepas, France). S. lycorpersicum M82 wild type were received from S. Biemelt, the la mutant (accession LA0335) from the Tomato Genetics Ressource Center and the gob mutants from D. Zamir. P. sativum (cv Térèse and line NGB5839) were obtained from C. Rameau and the uni mutant (line JI2171) from J. Hofer. S. tuberosum cv Pompadour were a gift from B. Letarnec. RNA extraction and RT-PCR RNA was extract from partially dissected apices or young leaves of the different species using TRIsol reagent (Invitrogen) according to the manufacturer recommendations. Contaminating DNA was removed by DNaseI treatment (Invitrogen) and the RNA was reverse transcribed (SuperScript TM II reverse transcriptase, Invitrogen). Gene specific primers used for semiquantitative RT-PCR are listed in Supplementary Table 4. PCR products were observed on ethidium bromide-stained gels or following southern blotting (PsNAM1/2).Cloning of NAM/CUC genes. ...

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