Haematopoetic effect of methanol seed extract of Citrus paradisi Macfad (grape fruit) in Wistar rats (original) (raw)
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2020
This research determined the effects of Citrus sinensis and Lycopersicon esculentum Miller juices on the hematological parameters of Rattus albus. To achieve its objectives, the study utilized quantitative-experimental method. For this purpose, nine, two-weeks-old male albino rat weighing 100-150g were used. Rats were kept under room temperature in a 12-hour alternate light and dark cycle and provided with free access to water and foods. Rats were divided into three equal groups namely the control group and experimental groups administered with 2ml of orange and 2ml of tomato juice respectively for 6 days for three continuous weeks. Blood were collected through saphenous vein sampling technique starting at the end of acclimatization and every 7th day of the week. The obtained blood samples were used for determination of hematocrit, red blood cell (RBC) count, hemoglobin, platelet count, white blood cell (WBC) and differential count including neutrophil, lymphocyte, monocytes, eosino...
Effect of Citrus aurantifolia juice on bodyweight and haematological indices of wistar rats
African Journal of Food Science
Citrus aurantifolia (Lime) is a citrus fruit and an excellent source of vitamin C and flavonoids which have unique antioxidant properties. This study determined the effects of C. aurantifolia on the bodyweight and hematological indices of rats. A total of 20 rats of both sex weighing between 120 and 130 g were randomized into 4 groups of five rats each and used. Group A: Normal Control; Group B: 2 ml/kg of C. aurantifolia fruit juice; Group C: 4 ml/kg of C. aurantifolia fruit juice; Group D: 8 ml/kg of C. aurantifolia fruit juice. The C. aurantifolia fruit was freshly squeezed and the juice was administered to the test groups for a period of three months. The haematological parameters were analysed using standard methods. The results revealed that administration of C. aurantifolia juice caused a significant difference (p<0.05) in the bodyweights of the experimented groups from week 3 up to week 12 compared with the normal control group. After three months of the administration, only the platelet counts showed a significant difference (p>0.05) in group B compared with other groups throughout the three months of administration. Therefore, it is then concluded that bioactive substances present in lime like lycopene and vitamin C induces the proliferation of white blood cells in the blood circulation. Hence, it was proven that lime has a protective effect which may serve as an alternative treatment option in patients with leukopenia.
Hematological Indices of Experimental Rats Fed with Rat Chow Fortified with Processed Breadfruits
Asian Journal of Biochemistry, Genetics and Molecular Biology
Background: The abundance of nutrients, vitamins, and fiber in breadfruit has been documented in many literature studies that can hardly be exhausted and are consumed majorly by the Igbos and other southern parts of Nigeria to ameliorate hunger. Aim: This study however was aimed at determining the effect of feeds fortified with grilled, preboiled, and cooked breadfruit on hematological indices of wistar rats. Methods: Hematological parameters were determined using an automated hematology analyzer (Mindray-BC-5300). The parameters analyzed include Hemoglobin (HGB), Packed Cell Volume (PCV), Red Blood Cells (RBC), Platelets (PLT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), White Blood Cells (WBC), Neutrophils (NEUT), Lymphocytes (LYMPH), Monocytes (MON), Eosinophils (EOS) and Basophils (BAS). Results: The result from the experiment indicated that all fortified groups showed no significant increase or decrease (p&...
2015
The effect of shaddock citrus fruit juice on some liver function markers, body weight and some hematological parameters of Wistar albino rats were evaluated in 16 male albino rats. The rats were procured from Faculty of Veterinary Medicine, Animal House, University of Nigeria, Nsukka and were of average age of 12weeks, weighing 130-170grams. The rats were acclimatized for 7days and randomly separated into 2 groups of eight rats each, kept in 2 different standard cages. The groups were as follows: Arats fed with rat feed (control), Brats fed with rat feed and shaddock juice. The study lasted for 28day. The rats were weighed with electronic weighing balance at weekly intervals. At the end of 28days feeding experiment, the rats were bled and blood collected used for liver and hematological parameters assays using standard biochemical methods. The result of the study indicated significant decrease (P<0.05) in the body weight gain of rats supplemented with shaddock juice on the 1 , 2 ...
Advances in Pharmacology & Clinical Trials
The present study evaluated the effect of methanol extract of the fruit peels of ripe plantain (an agricultural waste) on some haematological and biochemical indices in male Wistar rats. Phytochemical screening of the ripe peel extract was carried out using standard methods. The oral median lethal dose (LD50) was determined. Twenty-four (24) male Wistar rats were randomly assigned into 4 groups of 6 rats per group. Group 1 served as control. Groups 2-4 were administered 285.00, 570.00 and 855.00 mg/kg per body weight respectively of the ripe plantain peel extract for a period of 14 days. Blood samples were collected by cardiac puncture and analyzed for some hematological and biochemical parameters. Phytochemical screening of the ripe peel extract indicated the presence of flavonoids, tannins and cardiac glycosides. Acute toxicity study gave an LD50 of 2849.56 mg/kg. Treatment of experimental animals with the extract caused a dose dependent increase in white blood cell count (WBC), red blood count (RBC), packed cell volume (PCV) and hemoglobin concentration (Hb) in the medium and high dose groups. Total cholesterol, triglycerides, very low-density lipoproteincholesterol, low-density lipoprotein cholesterol and highdensity lipoprotein-cholesterol did not show any significant changes (p˃0.05) between control and treatment groups. Also, there were no significant changes (p˃0.05) in the serum activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase. These findings suggest that Research Article Volume 4 Issue 1
International Journal of Current Research in Biosciences and Plant Biology, 2016
The effects of extract of Citrus aurantifolia on some blood and liver parameters were examined. Graded doses (20,40,60,80 and 100 mg/kg) of extracts were administered daily by stomach intubation to rats for 14 days and the effects on body weight, liver enzymes and some blood parameters were evaluated. The results showed significant decreases in alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) when compared to the control group. Urea level also decreased significantly when compared with the control. The ALT (mMol/l) values had a range of 0.4 ± 0.1 to 5.1± 0.1 for the test groups, and 0.9± 0.9 as the control value. The AST (mMol/l) values had a range of 0.1 ± 0.28 to 0.2 ± 0.05 for the test groups, and 0.6±0.09 as the control value. The ALP mMol/l values had a range of 0.7 ± 0.13 to 1.1 ± 0.05 for the test groups, and 1.5±0.02 as the control value. Creantinine values had a range from 74.45 ± 21.07 to 262.65 ± 26.65 for the test groups, and a value of 78.0 ± 33.00 for the control group. The results show that the extract had no significant effects (p= 0.05) on liver enzymes and the blood parameters considered. There was a significant decrease in platelet counts and slight increases in packed cell volume (PCV), haemoglobin, white and red blood cells. Platelet counts (×10 9 /l) values ranged between 153.5 ± 1.50 and 275.0 ± 25.00 for the test groups and 275.0 ± 25.00 for the control group. PCV (%) values ranged from 38.0 ± 8.0 to 45.5±0.5 for the test groups, and 41.0 ± 0.00 as the control group value. Haemoglobin (mg/dl) values ranged between 12.5 ± 5.0 and 15.2 ± 0.17 for the test groups and, 13.6 ± 0.00 as the control. WBC (×10 9 /l) values ranges between 5.4 ± 1.2 and 6.85 ± 0.9 for the test groups and 5.3 ± 0.5 as the control. RBC (×10 9 /l) values also had a range of 5.15 ± 0.1 to 6.40 ± 0.1 for the test groups and 5.15 ± 0.2 for the control. These results suggests that the extract was not likely to produce toxic effects on the body system when given at normal therapeutic doses for a short-term, as evident in the liver enzymes levels and some blood parameters.
Haematological indices of albino rats fed diets supplemented with jackfruit seed (S), bulb (B) or seedbulb blend (SB) were assessed. Diets were compounded to include 10%, 30%, and 50% of the seed (S10/90, S30/70, S50/50), bulb (B10/90, B30/70, B50/50), and seed-bulb blend (SB10/90, SB30/70, SB50/50). Sixty male albino rats, divided into 10 groups of six rats each, were used. Group 1 (control) was fed rat chow only; groups 2-10 were fed the experimental diets. After 28 days of feeding, the animals were sacrificed and whole blood collected for full blood counts. The results, compared to the control (p<0.05), showed that supplementation exceeding S30/70 and B10/90 or SB10/90 showed significant (p<0.05) loss of body weight. Total WBC, lymphocyte and eosinophil counts were significantly (p<0.05) increased in the experimental groups. Hb was reduced in group B10/90 but RBC, Hb and PCV increased in B10/90 and SB10/90. The same trend was observed in MCV of groups B10/90 and B50/50. Platelet counts in the experimental groups were significantly similar to the control though lower in S30/70, B50/50 and SB50/50. Overall, data from this study suggest that diet supplementation with 10% jackfruit bulb or the seed-bulb blend is likely to provide some antianaemic benefits and enhance the immune system.
Bio-Research
The use of chemicals such as calcium carbide to ripen fruits instigates toxicity; inclusion of the chemically ripened fruits in the preparation of juices may induce blood cells depletion by altering hematopoiesis via declined functionality of erythropoietin, leukopoietin, thrombopoietin, interleukin-3, prostaglandins, hematopoietic stem cells; resulting to reduction in cellular components of blood. The blood cell depletion potentials of chemically ripened banana juice were investigated in 20 male Wistar rats categorized into four; Group A (control), Group B (administered naturally ripened banana juice), Group C & D (administered juices composed of 15mg/kg and 25mg/kg of calcium carbide ripened banana) (n=5). Following the administration protocol of the banana juice for 28days, data was obtained and statistically analyzed using GraphPad prism (version 8). Chemically ripened banana juices significantly decreased erythropoietin, leukopoietin, thrombopoietin, interleukin-3, prostaglandi...
Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia
Biosaintifika: Journal of Biology & Biology Education, 2017
Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing th...
Present study was performed on two combination doses of Citrus limon and Punica granatum i.e. 0.4ml/kg Citrus limon +5ml/kg Punica granatum (CPJ-1) and 0.2ml/kg Citrus limon +8ml/kg Punica granatum (CPJ-2) in healthy rabbits. CPJ-1 and CPJ-2 both showed significant increase in red blood cell count, hemoglobin, bleeding time and anticoagulation factors like protein C and thrombin antithrombin complex levels. While CPJ-1 cause significant increase in thrombin time and platelet count. However Fb was significantly decreased by CPJ-1. These results suggest that Citrus limon and Punica granatum has phytochemicals and other essential nutrients that possesses an anti-thrombin component, prevent thrombosis and plays cardio protective role.