Antioxidative properties of Mucuna nivea (Roxb.) DC by DPPH Assay (original) (raw)

Background: The present study was conducted to evaluate the antioxidative potential of leaves and seeds of Mucuna nivea due to presence of various bioactive phytoconstituents observed in preliminary phytochemical investigation. Method: A stable free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) was used as a standard oxidant to evaluate the antioxidant capacity of leaves and seeds extracted with five different solvents. Result: The qualitative data were obtained by confirming the presence of phytoconstituents, the five different solvents viz petroleum ether, chloroform, acetone, ethanol, and methanol were chosen by increasing order of their polarity. The significant percentage of inhibition of DPPH was observed in seed extracts of petroleum ether and methanol showing 46.33% and 45.76% respectively. However, the leaves extracted in petroleum ether reveals 30.91% of inhibition followed by 29.72% of inhibition were recorded in methanolic extract of leaves. Moreover, the ascorbic acid as a standard antioxidant shows 81.46% and 80.05% inhibition of DPPH in leaves and seed extracts of solvents respectively. Conclusion: From the observation, it can be concluded that the seeds and leaves are the good sources for antioxidants, for the further exploration of bioactive potential of M. nivea, the present investigation creates a basic platform for the future investigators.

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